Simultaneous determination of total human and male DNA using a duplex real-time PCR assay

A single duplex assay to determine both the amount of total human DNA and the amount of male DNA in a forensic sample has been developed. This assay is based on TaqMan technology and uses the multicopy Alu sequence to quantitate total human DNA and the multicopy DYZ5 sequence to quantitate Y chromosomal (male) DNA. The assay accepts a wide concentration range of input DNA (2 muL of 64 ng/microL to 0.5 pg/microL), and also allows detection of PCR failure. The PCR product sizes Alu (127 bp) and DYZ5 (137bp) approximate that of the smaller short tandem repeats (STRs) which should make the assay predictive of STR success with degraded DNA. The assay was optimized for probe/primer concentrations and BSA addition and validated on its reproducibility, on its human specificity, on its nonethnic variability, for artificial mixtures and adjudicated casework, for the effect of inhibitors and for state of DNA degradation. This assay should prove very usual in forensic analyses because knowing the relative amounts of male versus female DNA can allow the examiner to decide which samples may yield the most probative value in a case or direct the samples to methods that would yield the greatest information.     

Executive agencies in Tanzania: liberalization and Third World debt

The purchase of an expensive, debt‐financed aircraft tracking system by one of Tanzania's newly created executive agencies suggests that recent bureaucratic restructuring and liberalization in Tanzania could possibly undermine the good use of international debt management schemes for poverty alleviation. Moreover, it raises a question about the uses (and misuses), in countries where governance capacities are weak, of new public management models of organization. This article explores Tanzania's donor‐funded executive agency programme, as one part of its wider civil service reforms. Drawing on empirical research, it examines the power structures and interdependencies between Ministers, departments and NPM style semi‐autonomous agencies and the potential for rent‐seeking behaviour and perverse outcomes. Copyright © 2002 John Wiley & Sons, Ltd.