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The forensic community needs quick, reliable methods to quantitate human DNA in crime scene samples to replace the laborious and imprecise slot blot method. A real-time PCR based method has the possibility of allowing development of a faster and more quantitative assay. Alu sequences are primate-specific and are found in many copies in the human genome, making these sequences an excellent target or marker for human DNA. This paper describes the development of a real-time Alu sequence-based assay using MGB Eclipse primers and probes. The advantages of this assay are simplicity, speed, less hands-on-time and automated quantitation, as well as a large dynamic range (128 ng/microL to 0.5 pg/microL). 相似文献
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This article aims to contribute to the question of how to conceptualise the relationship between theory and practice in feminist
scholarship in law. It looks in detail at the implications of different issues raised in a recent debate between Anne Bottomley
and Ngaire Naffine on the existence of a “legal feminist orthodoxy”. I critique the dominance of ethics over politics and
join Bottomley in her attack upon “the ethics of respect for the other”, albeit from a different position. I then look at
the ways in which the problem of “essentialism” is being rethought from a feminist perspective. 相似文献
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Ramiro MartinezJr. Jacob I. Stowell Janice A. Iwama 《Journal of Quantitative Criminology》2016,32(3):471-488
Objectives
The temporal variation in homicide is examined by studying trends in race/ethnic specific killings (e.g. Blacks, Latinos and Whites). Two substantively important issues are also addressed—a closer examination of the role community heterogeneity plays in homicide levels and the treatment of immigration as an endogenous social process.Methods
Data are reported homicides in the city of San Diego, California over the period 1960–2010. The address of each killing is geocoded into 341 census tracts.Results
We find that neighborhoods experiencing increases in the foreign-born population tend to be less violent. White and Latino homicide victimization was reduced significantly as a product of increases in the neighborhood concentration of foreign-born individuals. Supplementary analyses did not find empirical evidence that the influx of foreign-born individuals could (or should) be considered a disruptive social process. Over the past five decennial census periods, the exponential increase in immigration in this border city is not associated with an increase in homicide victimization.Conclusions
When examined through a wider temporal lens than is typically employed, and accounting for the endogeneity of immigrant residential settlement, we find no support for the claims that immigration is a crime generating social process.60.
In this article, we empirically investigate the validity of the contract failure arguments as applied to a segment of the child care industry. However, prior research has focused upon actual quality differences between nonprofit and for-profit providers whereas the theory is concerned with consumers' perceptions of quality differences. In the current study, we examine both consumers′ perceptions and actual performance in testing the theory. In general, the data provide support for contract failure. 相似文献