Development of an Alu-based,QSY 7-labeled primer PCR method for quantitation of human DNA in forensic samples

Determining the amount of human DNA extracted from a crime scene sample is an important step in DNA profiling. The forensic community relies almost entirely upon a technique (slot blot) to quantitate human DNA that is imprecise, time consuming, and labor intensive. This paper describes the development of a new technique based on PCR amplification of a repetitive Alu sequence. Specific primers were used to amplify a 124-bp fragment of Alu sequence; amplification was detected by SYBR Green I staining in a fluorescent plate reader. To reduce background in the plate reader assay, QSY-7 labeled primers were utilized. The assay was tested on animal DNAs, human blood spots, mock crime samples, and degraded DNA in comparison with the slot blot technique. The QSY Alu assay has a dynamic range of 10 ng to 10 pg, and is sensitive, specific, fast, quantitative, and comparable in cost to the slot blot assay.     

On Not Making Ourselves the Prey of Others: Jean Hampton's Feminist Contractarianism

This article assesses Jean Hampton’s feminist contractarianism by considering the way in which she draws together the contradictory positions of Hobbes and Kant to produce a test for exploitation in personal relationships. The ways in which this work fits with her other analysis of retribution, gratitude and self-worth are examined. Hampton’s work is evaluated in the context of Carole Pateman’s argument that moral theories distract from the political analysis of who has a voice in relationships. Hampton’s work presumes the social and economic structures that Pateman has done so much to understand. It is useful as a claim for justice in personal relationships, to be considered as part of consciousness-raising or public debate.     

The Contribution of Emotion Regulation to Body Dissatisfaction and Disordered Eating in Early Adolescent Girls

To understand whether difficulties in emotional functioning distinguish between body dissatisfaction and disordered eating, a set of emotion regulation (i.e., negative emotion, emotional awareness, coping), demographic (i.e., age), and physical (i.e., BMI (Body Mass Index)) factors were assessed in 234 early adolescent girls, grades six to eight. Compared to younger girls, older girls had higher BMI and reported increased body dissatisfaction. Age, BMI, and negative affect predicted body dissatisfaction, whereas BMI, body dissatisfaction, and lack of emotional awareness predicted disordered eating. Further, girls who reported high levels of disordered eating reported experiencing increased levels of negative affect, greater difficulties with emotional awareness, and more difficulty coping constructively with negative emotion than girls who reported low levels of disordered eating. Results support the contention that body dissatisfaction, combined with difficulties in emotional awareness are related to disordered eating.Leslie Sim is an assistant professor at the Mayo Medical School, a Senior Associate at the Mayo Clinic, and Clinical Director of the Mayo Inpatient Eating Disorders Program. She received her Ph.D. from University of Maine in Developmental and Clinical Psychology. Her research interests include eating disorders, self-injurious behavior, and emotion regulation skills in children and adolescents.Janice Zeman is an associate professor at the College of William and Mary. She received her Ph.D. from Vanderbilt University in Developmental and Clinical Psychology. Her research interests include children's and adolescents' emotion regulation skills particularly as they relate to maladaptive functioning with other research interests in parental and peer socialization of emotion.     

Exploratory Factor Analysis and Reliability of the Child Welfare Trauma-Informed Individual Assessment Tool

This article presents the development and psychometric analysis of the Child Welfare Trauma-Informed Individual Assessment Tool, an instrument designed to measure the extent to which child welfare service providers employ trauma-informed practices with the children and families that they serve. Using the responses of 213 front-line child welfare workers, exploratory factor analysis was used on a seventeen-item scale, resulting in a four-factor model. Based on the results of this analysis, implications for potential uses of the instrument are discussed, and specifications for additional evaluation of the measure's validity are outlined.     

Bundaberg and beyond: duty to disclose adverse events to patients

Although much excellent work has been done in Australia and elsewhere to improve the safety and quality of health care provision, the practice of medicine is inherently risky--adverse events sometimes occur. In Australia, practical guidelines for the open disclosure of adverse events to patients have been developed and are being implemented. State and Territory medical boards have recently adopted Codes of Conduct which include disclosure provisions, although the Australian Medical Association's Code of Ethics does not yet contain express patient disclosure provisions. There is a dearth of authority concerning legal obligations to disclose known or suspected adverse events. Although many Australian jurisdictions have introduced statutory protection for those who apologise or express regret to patients following an adverse event, there is no corresponding express statutory disclosure obligation, unlike in some parts of the United States. The Bundaberg experience illustrates the complex ethical, practical and legal issues which arise in this area.     

John Gardner,in memoriam

Criminal Law and Philosophy -     

Parentage of Hydatidiform Moles

We were presented with the STR (short tandem repeat) profiles from two separate paternity trios. Each trio consisted of a mother, an alleged father, and products of conception (POC) that contained a hydatidiform mole but no visible fetus. In both cases , antecedent pregnancies had followed alleged sexual assaults. Mole classification and pathogenesis are described in order to explain the analyses and statistical reasoning used in each case. One mole exhibited several loci with two different paternal alleles, indicating it was a dispermic (heterozygous) mole. Maternal decidua contaminated the POC, preventing the identification of paternal obligate alleles (POAs) at some loci. The other mole exhibited only one paternal allele/locus at all loci and no maternal alleles, indicating it was a diandric and diploid (homozygous) mole. In each case, traditional calculations were used to determine paternity indices (PIs) at loci that exhibited one paternal allele/locus. PIs at mole loci with two different paternal alleles/locus were calculated from formulas first used for child chimeras that are always dispermic. Combined paternity indices in both mole cases strongly supported the paternity of each suspect.     

An Ethic of Care: A Valuable Political Tool?

Since Gilligan first interpreted women's moral position as an ethic of care, feminists have wondered what this means for political action. While some view it as a way of introducing forms of understanding and appreciation which are missing in the public sphere, others have worried about the universalisation and romanticization of women's abilities which it appears to contain. This paper argues that interpretations of an ethic of care which are situated in resistance and which conceptualise its abilities as the skills of subordinated groups can hold out visions of group solidarity of benefit to politics.