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Katie M. Horsman-Hall Yvette Orihuela Stephanie L. Karczynski Ann L. Davis Jeffrey D. Ban Susan A. Greenspoon 《Forensic Science International: Genetics Supplement Series》2009,3(4):242-250
Fired cartridge cases are a common type of evidence found at crime scenes. However, due to the high chamber temperatures and touch nature of this evidence, DNA testing is not commonly sought because it is believed DNA is only present in low levels, whether it is due to initial low levels of DNA and/or DNA degradation from the heat or inhibition of the PCR reaction. Moreover, very few laboratories report STR typing success with fired cases. This study focused on obtaining STR profiles from fired cartridge cases using the AmpFℓSTR® MiniFiler™ kit, which is designed to amplify DNA from low level, inhibited, and degraded samples. Comparisons to other STR amplification kits were also conducted. In attempt to simulate casework, random individuals loaded cartridges into a firearm. DNA was recovered from the fired cartridge cases using the double swab technique and extracted using an automated large volume DNA IQ™ method. Initially, testing focused on known shedders handling cartridges for 30 s prior to firing. A significantly greater number of alleles was obtained following amplification with the MiniFiler™ kit versus the PowerPlex® 16 BIO kit. No alleles were observed using the Identifiler® kit. In an attempt to better simulate casework, a random selection of laboratory personnel handled shotshells for as long as needed to load and fire the weapon. In this mock sample study, the MiniFiler™ kit successfully amplified an average of 22% of expected alleles from DNA recovered from shotshell cases versus the PowerPlex® 16 BIO kit where an average of 7% of alleles were observed. However, the total number of alleles obtained from the two kits was not significantly different. The quality of the DNA obtained from fired cases was studied with evidence of inhibition in at least 11% of shotshell case samples. After swabbing the head and the hull of three shotshell cases separately, a significantly greater number of alleles was obtained from the hull as opposed to the head of the fired shotshell case. In addition, after firing, various internal firearm surfaces were swabbed, including the chamber of barrel, ejection port, and breechface, in an attempt to obtain amplifiable DNA. DNA was obtained from the chamber of the barrel and was amplifiable using the MiniFiler™ kit, although mixtures were obtained with extensive drop-in and drop-out making this analysis unlikely to aid an investigation. 相似文献
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The existing model of political competition is extended to allow voters to live in different regions and to migrate between regions in response to an inter-regional transfer policy. We then show that regions have a different ``weight'' in the expected vote function of political parties. This gives parties an incentive to bias the transfer policy in favour of relatively high weight regions, with potentially adverse efficiency and equity effects. However, we then show that parties always propose efficient and equitable regional transfers, regardless of whether regions have different weights, if there is some mobility of citizens across regions. Mobility constrains parties to act efficiently and equitably even though they face an incentive to act otherwise. However, when voters are immobile political competition leads to inequitable though efficient outcomes. 相似文献
977.
Following approval of a referendum in 1993, New Zealand replaced its first–past–the–post electoral system with proportional representation (PR). Although support for PR was initially high, less than a third expressed support for the new system a year and a half after its implementation. We examine two explanations for this decline. One theory assumes that dissatisfaction with the new system is the result of a growing alienation with politics, exacerbated by an unpopular coalition government that voters neither expected nor desired. Another theory assumes that evaluations of the new system are mediated by a preference for coalition or single party government. Our results indicate that a preference for single party government, guided primarily by partisan self–interest, has the largest impact. Nevertheless, negative evaluations of the performance of the coalition government helped contribute to a loss in support for PR suggesting that government performance can affect citizen's evaluation of political institutions, particularly when systems undergo radical change. 相似文献
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Mbilinyi LF Zegree J Roffman RA Walker D Neighbors C Edleson J 《Journal of family violence》2008,23(5):343-351
Although voluntary enrollment by abusive men in domestic violence perpetrator treatment programs occurs, most men enter treatment
only after they have injured a partner or family member and have been arrested, convicted and sentenced. This leaves a serious
gap for those who engage in abusive behavior but who have not been served by the legal or social service systems. To address
this gap, the researchers applied social marketing principles to recruit abusive men to a telephone-delivered pre-treatment
intervention (the Men’s Domestic Abuse Check-Up—MDACU), designed to motivate non-adjudicated and untreated abusive men who
are concurrently using alcohol and drugs to enter treatment voluntarily. This article discusses recruitment efforts in reaching
perpetrators of intimate partner violence, an underserved population. Informed by McGuire’s communication and persuasion matrix,
the researchers describe three phases of the MDACU’s marketing campaign: (1) planning, (2) early implementation, and (3) revision
of marketing strategies based on initial results. The researchers’ “lessons learned” conclude the paper.
This project is supported by a grant from the National Institute on Drug Abuse, 1 RO1 DA017873. 相似文献