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41.
The majority of microfluidic devices used as a platform for low‐cost, rapid DNA analysis are glass devices; however, microchip fabrication in glass is costly and laborious, enhancing the interest in polymeric substrates, such as poly (methyl methacrylate) (PMMA), as an inexpensive alternative. Here, we report amplification in PMMA polymerase chain reaction (PCR) microchips providing full short tandem repeat profiles (16 of 16 loci) in 30–40 min, with peak height ratios and stutter percentages that meet literature threshold requirements. In addition, partial profiles (15 of 16 loci) were generated using an ultrafast PCR method in 17.1 min, representing a ~10‐fold reduction in reaction time as compared to current amplification methods. Finally, a multichamber device was demonstrated to simultaneously amplify one positive, one negative, and five individual samples in 39 min. Although there were instances of loci dropout, this device represents a first step toward a microfluidic system capable of amplifying more than one sample simultaneously. 相似文献
42.
Careful users of CMP party position data should take the uncertainty of position estimates into account. We compare and evaluate two current approaches that provide error estimates for party positions. Researchers of the CMP group identify measurement error in quantitative content analysis as the cause of uncertainty about position estimates, whereas a second approach by Benoit et al. (2009) attributes the uncertainty of position estimates to a stochastic generation of election programs. We illustrate the commonalities and differences of these approaches and provide two empirical applications, the identification of the left–right order of parties and of policy shifts by parties, using CMP data for 25 countries. Despite conceptual differences, results in these applications are surprisingly similar. 相似文献
43.
Within the context of acute public spending cuts and the increasing push towards localism, the UK government is increasingly looking outwards to community- and citizen-led action for solutions to long-term social problems and to take on public services. The extent to which these groups have the capacity and willingness to take on politicised roles beyond their purpose and function is, however, not well understood. By reflecting on findings primarily from a street-level mapping project, in this paper discussion focuses on the potential implications arising from grassroots' co-option.La société civile au niveau de la base populaire à la croisée des chemins : rester sur le chemin de l'indépendance ou prendre la voie du gouvernement britannique vers le localisme ?Dans le contexte des profondes coupes sombres opérées dans les dépenses publiques et de la poussée croissante vers le localisme, le gouvernement britannique se tourne de plus en plus vers l'extérieur, vers des actions menées par les communautés et les citoyens pour résoudre les problèmes sociaux à long terme et se charger des services publics. La mesure dans laquelle ces groupes ont les capacités et la volonté nécessaires pour assumer des rôles politisés qui dépassent leur finalité et leur fonction n'est pas, toutefois, bien comprise. En réfléchissant sur les constatations faites principalement dans le cadre d'un projet de cartographie au niveau des rues, la discussion de cet article porte sur les implications potentielles de la cooptation de la base populaire.La sociedad civil de base en la disyuntiva: ¿mantener el camino de la independencia o tomar la ruta del gobierno del Reino Unido hacia el localismo?En un contexto de severos recortes en el gasto público y de creciente impulso hacia el localismo, el gobierno del Reino Unido está fomentando cada vez más que las comunidades y los ciudadanos impulsen acciones tendientes a solucionar longevos problemas sociales y que se hagan cargo de los servicios públicos. Sin embargo, no se comprende bien hasta qué punto dichos grupos tienen la capacidad y la disposición de asumir roles politizados más allá de sus objetivos y de su función. A partir de reflexiones en torno a las conclusiones provenientes principalmente de un proyecto de mapeo a nivel de calle, el presente artículo se centra en analizar las posibles implicaciones que podrían surgir de la cooptación de los grupos de base.Sociedade civil de base na encruzilhada: permanecendo no caminho para a independência ou voltando-se para a rota do governo do Reino Unido em direção ao localismo?Dentro do contexto de significativos cortes nos gastos públicos e um empenho crescente em direção ao localismo, o governo do Reino Unido está cada vez mais olhando para fora para ações coordenadas pela comunidade – e cidadãos – para soluções de problemas sociais de longo prazo e contratação de serviços públicos. Porém, não se sabe muito bem até que ponto estes grupos têm capacidade e disposição de adotar funções politizadas além de seu propósito e função. Ao refletir sobre os resultados obtidos principalmente a partir de um projeto de mapeamento realizado nas ruas, a discussão deste artigo concentra-se nas implicações em potencial que surgem da opção conjunta de base. 相似文献
44.
The Qualified Lawyers Transfer Scheme (QLTS) provides a route for lawyers from other jurisdictions and barristers from England and Wales to qualify as solicitors in England and Wales. One of the three tests in the QLTS, the Multiple Choice Test (MCT), uses multiple choice questions to examine the syllabus of the qualifying law degree together with some pervasive subjects from the Legal Practice Course. This paper examines the MCT in detail. Particular attention is paid to the format and structure of the questions. We describe the detailed editing which each question goes through before it is used, the statistical analysis and review which take place after an exam, and the rationale for these processes. An explanation is given of what reliability and accuracy mean and how they are measured statistically. There is also an explanation of the “Angoff method” by which pass marks are set. Finally, the paper reports on the first four sittings of the MCT and their statistical results including their reliability and accuracy. Use of the multiple choice test, though well established for examining applied knowledge in medicine and in law in other jurisdictions, is a radical departure for the assessment of law in England and Wales. The experience of QLTS has shown that a carefully constructed multiple choice test of a suitable length can assess the qualifying law degree content both reliably and accurately. 相似文献
45.
Miranda Ritchie Katherine Nelson Russell Wills Lisa Jones 《Journal of family violence》2013,28(5):471-477
This study aimed to determine whether training and documentation led to improved assessment of female victims of assault due to domestic violence presenting to an emergency department. Eighty clinical records were audited using a valid and reliable audit tool over four time periods for variables including history, risk assessment, and examination. Documentation differed across the four groups (p?=?0.011). Between group analysis found there was a difference (p?=?0.055) between Groups 1 (before training) and 2 (after training), and Group 3 (with a specialist form in place), and there was a difference (p?=?0.009) between Groups 1 and 4 (8 years later). Training alone did not lead to change. Introducing specialist documentation with training was associated with an improvement in the standard of clinical assessment of female victims of assault due to domestic violence and this change increased over time. 相似文献
46.
Jenny de Fine Licht 《Public administration review》2014,74(3):361-371
Building on the counterintuitive findings of recent empirical studies that transparency in political decision making may have a negative effect on public legitimacy beliefs, this article suggests that transparency has different effects depending on the policy area. Specifically, it argues that transparency is less effective in policy decisions that involve trade‐offs related to questions of human life and death or well‐being. Using an experiment that involved 1,032 participants, the effect of transparency is tested in two policy areas that represent routine priority setting (culture and leisure) and policy decisions implicitly related to human life and well‐being (traffic security). Results indicate that transparency can increase public acceptance of political decisions, but this effect is moderated by the type of policy area. Furthermore, a limited type of transparency in which decision makers provide justifications for their decisions can result in benefits while avoiding potential costs. 相似文献
47.
This paper describes a simple processing and analysis scheme for explosives trace swab samples which deals both with organic and inorganic materials. Swabs, wetted with ethanol or ethanol/water mixture, were extracted with ethanol/water mixture. The extract was passed directly through a simple column containing an acrylonitrile/styrene copolymer adsorbent. The adsorbent retained common organic explosives, which were recovered with an efficiency of 30-50% as a relatively clean ethyl acetate solution. The concentrated ethyl acetate eluate was analysed using gas chromatography with chemiluminescence or mass spectrometric detection. The unretained inorganic ions and sugars, which were recovered with generally high efficiency as an ethanol/water solution, could be directly analysed using ion chromatography and/or capillary electrophoresis. Minor difficulties encountered in the analysis of sugars, fluoride and phosphate were examined. 相似文献
48.
Deliberation is an essential element in legitimate and sound decision making. The deliberative ideal has much resonance with ideas of ‘localization’, employing the value of local and applied knowledge. Participation is also of particular value under globalization. We argue that the capacity of the World Trade Organization (WTO) to absorb and reflect participatory aspects of decision making is crucial to its future legitimacy and status. Should the WTO be seen as one of the darker forces of globalization? Or as an emerging institution of global accountability? The latter depends upon recognition that the potential deregulatory effect of the WTO is contingent, and that the liberalization of international trade should enhance welfare, rather than be a goal in its own right. Deliberative solutions require a strong public sphere, and we therefore consider whether solutions based on ‘empowered consumer choice’, rather than public deliberation, are unsatisfactory responses to the deregulatory impact of international trade disputes and their outcomes. 相似文献
49.
Yourstone J Lindholm T Kristiansson M 《International journal of law and psychiatry》2008,31(4):374-383
The purpose of this study was to compare the psychosocial background of female and male perpetrators convicted of homicide in Sweden between 1995-2001. All women (n=43) who were convicted for lethal violence during the period and a corresponding number of randomly chosen men (n=43) were examined. In total, information about 86 individuals was collected retrospectively. Areas of interest were psychosocial variables during childhood and at the time of the current crime. Results showed that both female and male perpetrators were psychosocially encumbered already at an early age. Homicidal women had more severe childhood circumstances, but less aggressive childhood behaviour than did their male counterparts. At the time of the crime, women had a more ordered social situation, had more often been exposed to violence and searched for help than had the men. These gender differences suggest that specific actions are needed for preventing women's homicidal behaviour. 相似文献
50.
ABSTRACTGiven the growing complexity in British policing, the College of Policing are implementing a Police Education Qualification Framework through a professionalization agenda. This aims to standardise entry to the police and allow serving officers to gain accreditation for their previous training and experience. Part of this process involves the development of a national police curriculum for higher education institutions to deliver to new recruits. Different definitions of what constitutes professionalism can impact on officers’ interpretations of this concept and how they subsequently engage with the proposed reforms. This paper, which is based on in depth qualitative interviews with serving officers who have undertaken an academic qualification in policing, suggests that the relationship between police education and the development of professionalism is complex. Officers need to be trusted and encouraged to use their learning in a way that develops their own personal sense of professionalism. However, this paper will argue that current perceptions amongst officers are sceptical of the wider agenda and brings into question the development of a standardised curriculum which may ultimately be viewed as further governance over officer behaviour. 相似文献