Expedited, chemically enhanced sperm cell recovery from cotton swabs for rape kit analysis

This report focuses on the development of a method for chemically induced enhancement of cell elution and recovery from cotton swabs. The method exploits the exclusive use of detergents for intact cell removal, and can be utilized in conjunction with, or to circumvent, conventional differential extraction (DE). Samples treated with Sarkosyl (54.4 +/- 1.8%) and sodium dodecyl sulfate (SDS) (78.5 +/- 0.7%) yielded higher sperm cell recoveries than a conventional DE buffer (39.4 +/- 2.1%). The results indicated that the choice of detergent affected sperm cell yield, with anionic detergents having the greatest effect. Storage time of samples affected the concentration of detergent required for optimal sperm cell recovery, longer times requiring increased detergent concentrations. In addition, the extent of sperm cell lysis by proteinase K digestion was evaluated. The results indicate that the exclusive use of SDS enhances the release of sperm and epithelial cells from a cotton swab as compared with DE buffer, providing for a more effective DNA analysis.     

The Role of “Problematic” and “Improved” Indicators of Risky Lifestyles in the Self-Control/Lifestyle Framework of Victimization Among Filipino Adolescents

Pratt and Turanovic (European Journal of Criminology, 13(1):129–146, 2016) argue that previous studies operationalizing risky lifestyles as mere “going out” (problematic indicators of risky lifestyles) were misspecified and that “improved” indicators of risky lifestyle (risky behaviors) would perform better than “problematic” indicators in models that explain victimization. This study examines these propositions by testing the self-control/lifestyle framework of victimization using the data from a random sample of Filipino high school students at a state university in Dumaguete City, Philippines. Results show strong support to Pratt and Turanovic’s claims. Self-control has stronger effects on improved indicators than on problematic ones. And, improved indicators have stronger effects than problematic indicators on property, violent, peer/sibling and sexual victimization. Moreover, the findings provide partial support for the self-control/lifestyle framework of victimization.