Development and usage of a NIST standard reference material for real time PCR quantitation of human DNA

National Institute of Standards and Technology SRM 2372 human DNA quantitation standard has been produced to support the need for a human-specific DNA quantitation standard in forensic casework and calibration of new quantitative polymerase chain reaction (qPCR) assays. The conventional DNA concentration has been assigned with one of the U.S. National Reference UV/Visible Spectrophotometers, assuming an absorbance of 1.0 at 260 nm equals 50 ng/μL of double stranded DNA. In addition, an interlaboratory study has been conducted, to verify that the SRM 2372 materials perform well in currently used DNA quantitation assays by the forensic DNA community. Each unit of SRM 2372 consists of three well-characterized DNA extracts. Component A is a single-source human male material derived from blood. Component B is a multiple-source human female material derived from blood. Component C was purchased as a purified unsheared genomic human DNA (Sigma-Aldrich Co., St. Louis, MO) obtained as a lyophilized human genomic extract and has both male and female donors. SRM 2372 is intended to enable the comparison of DNA concentration measurements across time and place. Manufacturers can use SRM 2372 to validate the values assigned to their own reference materials. Individual forensic laboratories can use SRM 2372 to validate DNA quantitation methods and to verify the assigned concentration of in-house or commercial DNA calibration standards.     

Profiles of Victimized Women Among the Child Welfare Population: Implications for Targeted Child Welfare Policy and Practices

Despite the call to address domestic violence along with child maltreatment, little information exists to guide services for victimized women involved with child welfare. Research shows that victimized women contend with multiple problems stemming from violent victimization. Unfortunately less is known about combinations of needs and resources among victimized women, especially for those involved with child welfare. Through an examination of needs and resources among families with child maltreatment and domestic violence, we aimed to help address this knowledge gap. Needs and resources among 1,229 victimized caregivers were examined using the National Survey of Child and Adolescent Well-being (NSCAW). Using latent class analysis, four distinct multivariate profiles of needs and resources among victimized caregivers were identified. Significant differences were found among the profile groups in the family violence they experienced in the 18 months following child welfare investigations. Based on these findings, we discuss implications for tailored practices to enhance the safety of victimized caregivers involved with child welfare. An earlier version of this paper was presented at the 9th Annual Conference of the Society for Social Work and Research, Miami, Florida in January 2005.     

Is there a magical time boundary for diagnosing eyewitness identification accuracy in sequential line‐ups?

We examined whether eyewitness identification latencies for sequential line‐up decisions indicate an optimum time boundary that reliably discriminates accurate from inaccurate decisions. Participants (N = 381) observed a crime simulation and attempted two separate identifications from target‐present or target‐absent sequential line‐ups. As has previously been found with simultaneous line‐ups, the optimum time boundary identified did not reliably discriminate accurate from inaccurate identifications for both line‐up targets. Diagnosticity for choosers was, however, much higher at very high confidence levels than at lower levels. Possible reasons for why one index of signal strength (confidence), but not another (latency), might postdict accuracy within the sequential framework were presented.     

SOCIAL SECURITY RETIREMENT BENEFITS The Last Insult of a Sexist Society

An older, divorcing couple rarely has assets worth more than their Social Security rights. After a house and pension/retirement plans, Social Security is their largest "asset." Yet, it is rarely considered by lawyers, mediators, or the parties themselves. A key point, often missed, is that the working spouse receives twice as much Social Security as the nonworking spouse. For many retiring women, getting half as much Social Security as their ex-husbands is just one more insult from a sexist society. Although a nonworking husband will get the same half, the problem is largely one affecting older women, who earned less on average while they worked and who usually took time off to be with the children while the children were growing up. This article looks at Social Security regulations that make this so and how to avoid this unjust result.     

茜草素的抗菌活性与药效试验

用化学合成的茜草素做了体外抑菌试验、体内抗菌活性试验、人工感染猪多杀巴氏杆菌疗效试验、急性毒性试验及临床治疗试验。结果表明,茜草素在体外对金黄色葡萄球菌、变形杆菌、大肠埃希氏菌、绿脓杆菌、沙门菌和克雷伯氏菌等均有显著的抑制作用,对后4种病原菌的抑制作用超过了六茜素。在小白鼠体内对大肠埃希氏菌也有非常显著的抗菌作用。对人工感染多杀巴氏杆菌的患病仔猪有显著的治疗作用。小白鼠腹腔注射茜草素的LD50为336 mg/kg。茜草素对临床型乳牛乳腺炎的治愈率为87.5%,总有效率为100%。