Optimization of recovery of human DNA from envelope flaps using DNA IQ™ System for STR genotyping

An optimized protocol based on the DNA IQ™ System has been tested for the extraction of DNA from envelope flaps. DNA is extracted directly without the need for opening and swabbing the flaps. The method is repeatable with <10% R.S.D. (relative standard deviation). The results of a systematic study show that it is an equilibrium extraction, and a small sample volume as well as high lysis buffer content in sample contribute to high extraction efficiency. The extracted DNA requires no further purification steps following its extraction with the DNA IQ™ System. Complete but skewed 15-locus short tandem repeat (STR) profiles, which is typical of degraded of DNA, have been generated from the DNA extracted from 6 to 9 years old casework envelope samples.     

SEPARATION ANXIETY AND DE FACTO DENIAL OF VISITATION

The dynamics of separation anxiety and its potential impact upon restriction of legal visitation in preschool age children is discussed. The interplay between adult personality characteristics and the child's developmental level is inextricably intertwined to generate separation fears. This interplay is presented within a theoretical framework of interaction. Unfortunately, the child's reactions to separation are frequently misinterpreted by the parents as indications of some mistreatment by the former spouse. Although adjustment difficulties in preschool age children coping with divorce are likely, the pattern of disturbance associated with separation anxiety is predictable and occurs almost exclusively with temporary removal from the primary parent. Behavioral health and legal professionals are cautioned against allowing such behavioral disturbance to be utilized by combative parents to deny, restrict or abridge scheduled visitations with the nonresidential parent. Requests for private custody evaluations have increased dramatically in the past decade. Behavioral health professionals often find themselves being jointly stipulated to by disagreeing parties, or directly appointed by the court, to help formulate a residential plan to meet “the best interests of the children” affected. When assessing divorcing families with young children, varied adjustment patterns are observed. However, as Wallerstein and Kelly (1980) discovered, almost all children are adversely affected by the family rupture, at least on a short-term basis. Surprisingly less than 10 percent of the children experienced relief following the parental decision to divorce, despite the degree of explosiveness which may have characterized the dysfunctional marital relationship. Further, the particular response style exhibited by the children was primarily dependent upon the developmental and emotional level the child attained prior to the time of divorce. While positive adjustment has been correlated with an absence of feuding or animosity between the divorcing parents (McKinnon & Wallerstein, 1986), preschool children seem quite vulnerable to adjustment difficulties. Evidence has also begun to accumulate to suggest that divorce has a more serious impact upon boys than girls (Hetherington, 1979). Problems frequently surface during times of exchange, particularly when parents are actively engaged in battle. At its worst, these extreme maladjustments may resemble what appears to be a separation anxiety. Again, the Wallerstein and Kelly (1980) studies reported that preschool age youngsters displayed fright, exhibited regressions, confusion, increased aggression, maintained disruptive fantasies, and experienced guilt. While most of these symptoms remit within a year to a year and a half following divorce, continued bickering and conflict between the parents either prevents resolution, or may even exacerbate, these symptoms later. The purpose of this paper is to discuss the phenomenon of separation anxiety in greater detail, and to provide professionals aiding divorcing families with preschool age children sufficient information to recognize and understand these problems. Hopefully, this knowledge will facilitate those entrusted with making custodial-visitation recommendations, the confidence to follow through with agreed visitation plans despite what at first may appear to be discomfort to the child.     

Maritime Security in East Asia and Prospects for U.S.-China Cooperation

As China and the United States explore how to craft a ＂new type＂ .or ＂new model＂ of bilateral relations, a key challenge will be deepening cooperation on a series of shared security issues. Alongside problems on the Korean Peninsula, cyber security, and other matters, is preserving peace and stability in East Asia＇s maritime regions, especially in the context of ongoing disputes in the Yellow, East China, and South China seas.     

Political context,policy networks and policy change: the complexity of transition in Hong Kong

Abstract

Central to the debates on the transition of Hong Kong to Chinese sovereignty is how this process has affected change in the policy process and policy outputs. Many see policy change as a result of the evolving political environment in Hong Kong following the political transition. This article, however, adopts the notion of policy networks and argues that the analysis of policy change cannot be reduced to a simple contextual stimulus – the policy alteration model. A case study – ‘the development of civic education’ – demonstrates the importance of policy networks, as a particular structure of government and group relations in decision making, in explaining the course of policy change. It is apparent that the relationship between regime change and political liberalization, on the one hand, and established networks, on the other, tends to be complex and dialectical in Hong Kong. Despite the importance of sovereignty transition and political restructuring, the effect of contextual factors on public policy greatly depends on the nature of the network involved.     

China–Taiwan diplomatic competition and the Pacific Islands

Abstract

China and Taiwan have become important actors in South Pacific affairs due to their diplomatic rivalry. Securing the diplomatic recognition of the Pacific Islands countries is expensive for China and Taiwan. There are limits to what the rivals are willing to spend, and they attempt to reduce costs. This dynamic shapes how Taiwan and China engage Pacific Islands politicians. It also motivates their high level official visits to the region, and how they engage South Pacific regional organizations. Despite criticisms that China–Taiwan rivalry corrupts and destabilizes the South Pacific, the issue of whether China and Taiwan's diplomatic rivalry has been beneficial or detrimental to the region remains contentious. China and Taiwan appear to have recently called a truce in their decades-old rivalry. This tacit agreement is still tentative, and the involvement of China and Taiwan in the region has yet to change significantly. However, Taiwan has reportedly begun to reduce funding, and is likely to reform its aid delivery in order to satisfy demands from the South Pacific region's dominant power, Australia, and to improve its image as a humanitarian aid donor. China is also likely to reduce funding while the truce holds. However, China considers its ties with South Pacific governments more important than responding to Australian pressure, and is unlikely to reform its South Pacific aid programmes as a result of the diplomatic truce.     

Book Reviews

Arran Gare: NIHILISM, INC.: Environmental Destruction and the Metaphysics of Sustainability. Como, New South Wales, Australia: Eco‐Logical Press, 1996.

David Harvey: Justice, Nature and the Geography of Difference. Oxford: Blackwell, 1996.

David Macauley, editor: Minding Nature: The Philosophers of Ecology. New York: The Guilford Press, 1996.

Daniel M. Herman and John T. O'Connor: Who Owns the Sun? People, Politics, and the Struggle for a Solar Economy. White River Junction, VT: Chelsea Green Publishing, 1996.

Harold Crooks: Giants of Garbage: The Rise of the Global Waste Industry and the Politics of Pollution Control. Toronto: Lorimer, 1993

Sudhir Chella Rajan: The Enigma of Automobility: Democratic Politics and Pollution Control. Pittsburgh, PA: University of Pittsburgh Press, 1996.

Garrett Hardin: Living Within Limits: Ecology, Economics, and Population Taboos. New York: Oxford University Press, 1993.