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排序方式: 共有375条查询结果,搜索用时 31 毫秒
51.
52.
Véronique Bourdon Ph.D. Carolyn Ng B.S. Jessica Harris M.S. Mechthild Prinz Ph.D. Eli Shapiro Ph.D. 《Journal of forensic sciences》2014,59(4):1057-1063
Sequencing mitochondrial DNA hypervariable regions I and II (HVI and HVII) is useful in forensic missing person and unidentified remains cases. Improvements in ease and sensitivity of testing will yield results from more samples in a timely fashion. Routinely, amplification of HVI and HVII is followed by Sanger sequencing using the BigDye® Terminator v3.1 Cycle Sequencing kit (Applied Biosystems) using 4 μL of ready reaction mix (RRM). Each sequencing reaction is then purified through column filtration before capillary electrophoresis. Using lower amounts of RRM (2 μL or 1 μL) and purification using BigDye® XTerminator? (Applied Biosystems) instead of columns showed no loss of sequence length and increased the quality and the sensitivity of testing, allowing HVI and HVII typing from mitochondrial genome equivalent to 125 fg of nuclear DNA, or 100 pg of HVI/HVII amplicons. Using this methodology, testing can be completed in 1 day, and the cost of testing is reduced. 相似文献
53.
This article will consider the following questions: What is the current state of the European data privacy and transfer regime? What are the challenges the regime is facing and what are the modifications being considered to address them? What role can the Sedona Conference play in reconciling personal data regulations and cross border discovery? We will explore the following critical issues: 1. The position taken by the European Commission (EC) in its proposed General Data Protection Regulation (2012) that the current regime, as embodied in the European Union (EU) Data Protection Directive 95/46/EC, has not fully achieved the harmonization of its member state's legal systems that was originally envisioned; 2. How the regime can adapt to the challenges and be driven by dynamic legal, technological and economic environments? The paper will conclude by examining key provisions of the EC's proposed regulation within the context of the Sedona Conference's International Principles and offer recommendations on implementation. 相似文献
54.
Joel Wuthnow 《中国国际问题研究》2014,(3)
The cooperation in maritime security is an integral part for the building of a "new type" or "new model" of great power relations. In this paper, the author investigates the causes of the maritime insecurity in East Asia, and introduces a number of practical ways through which China and the U.S.can consider enhancing cooperation in this area. 相似文献
55.
Eric L. Piza Joel M. Caplan Leslie W. Kennedy 《Journal of Quantitative Criminology》2014,30(2):237-264
Objectives
Despite the popularity of closed circuit television (CCTV), evidence of its crime prevention capabilities is inconclusive. Research has largely reported CCTV effect as “mixed” without explaining this variance. The current study contributes to the literature by testing the influence of several micro-level factors on changes in crime levels within CCTV areas of Newark, NJ.Methods
Viewsheds, denoting the line-of-sight of CCTV cameras, were units of analysis (N = 117). Location quotients, controlling for viewshed size and control-area crime incidence, measured changes in the levels of six crime categories, from the pre-installation period to the post-installation period. Ordinary least squares regression models tested the influence of specific micro-level factors—environmental features, camera line-of-sight, enforcement activity, and camera design—on each crime category.Results
First, the influence of environmental features differed across crime categories, with specific environs being related to the reduction of certain crimes and the increase of others. Second, CCTV-generated enforcement was related to the reduction of overall crime, violent crime and theft-from-auto. Third, obstructions to CCTV line-of-sight caused by immovable objects were related to increased levels of auto theft and decreased levels of violent crime, theft from auto and robbery.Conclusions
The findings suggest that CCTV operations should be designed in a manner that heightens their deterrent effect. Specifically, police should account for the presence of crime generators/attractors and ground-level obstructions when selecting camera sites, and design the operational strategy in a manner that generates maximum levels of enforcement. 相似文献56.
57.
Peter Bowal Kathryn Kisska-Schulze Richard Haigh Adrienne Ng 《American Business Law Journal》2020,57(4):677-733
This article explores cannabis regulation in Canada and the United States and examines how recent legalization efforts have manifested differently in each country. Although some of these differences are explained by uncompromising constitutional structures, this comparative exercise examines each system's political choices. While emphasis is placed on the Canadian experience, this article seeks to inform and enhance policy and regulation in both countries in addition to highlighting inherent ethical issues. 相似文献
58.
Joel Wuthnow 《Asian Security》2018,14(3):230-245
Chinese President Xi Jinping has touted an Asian security architecture in which “it is for the people of Asia to run the affairs of Asia.” But does China really want to exclude the United States from the regional order? This article argues that previous answers are often insufficient because they do not account for sub-regional variation in China’s strategy. In maritime Asia, China seeks a significantly reduced role for the US and its alliances though major constraints limit the prospects for success. In continental Asia, however, the situation is more nuanced, with Beijing alternately ignoring, supporting, or hedging against US presence. The policy implication is that Washington should not overstate Beijing’s role as either a regional adversary or a regional partner. Rather, the United States needs to approach China on its own terms across sub-regions. 相似文献
59.
60.
Identification and Individualization of Lophophora using DNA Analysis of the trnL/trnF Region and rbcL Gene
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Adrienne E. Ng B.S. Ernesto Sandoval B.S. Terence M. Murphy Ph.D. 《Journal of forensic sciences》2016,61(Z1):S226-S229
Lophophora williamsii (peyote) is a small, spineless, greenish‐blue cactus found in Mexico and the southwestern United States. Ingestion of the cactus can result in hallucinations due to its content of mescaline. In the United States, L. williamsii is classified as a Schedule I controlled substance. In this study, we use DNA analysis of the chloroplast trnL/trnF region and chloroplast rbcL gene to identify the individuals of Lophophora. Using the rbcL gene, Lophophora specimens could be distinguished from outgroups, but species within the genus could not be distinguished. The trnL/trnF region split the Lophophora genus into several groups based on the length and substructure of an AT‐rich segment of the sequence. Our results indicate that the genetic variability at the trnL/trnF locus is greater than previously recognized. Although DNA structures at the trnL/trnF region and rbcL gene do not align with the classification of Lophophora species, they can be used to aid in forensic analysis. 相似文献