人肺癌中TNF-α基因多态性分布的意义及其对细胞凋亡的影响

目的:观察TNF-α基因启动子区-308位核苷酸多态性基因型在肺癌和对照人群中的分布,以及这种多态性对该基因表达和细胞凋亡的影响。方法:PER-RFLP分析112例肺癌和99例正常人的TNF-α基因启动子区-308位核苷酸多态性,TNF-α-308*G等位基因记做TNF-α1,TNF-α-308*A等位基因记做TNF- α2。免疫组织化学方法检测肺癌组织中TNF-α的表达,比较不同基因型肺癌病例的标本之间TNF-α基因表达的差异性。TUNEL法检测肺癌组织中细胞凋亡,并对比分析TNF-α-308位核苷酸多态性及其对肺癌组织中TNF -α表达和不同类型细胞凋亡的影响。结果:肺癌组与对照组的基因型分布为TNF-α1/1(52．7％、46．5％)。 TNF-α1/2(35．7％、48．5％),TNF-α2／2(11．6％、5．1％);等位基因频率为TNF-α1(70．5％、70．7％),TNF- α2(29．5％、29．3％),组间没有显著性差异,P>0．05。两种基因型个体在TNF-α基因表达上,TNF-α2/2基因型个体高于TNF-α1／1基因型的个体,两者差异显著,P<0．01。TNF-α表达的高低与癌细胞凋亡有比较明确的关系,TNF-α表达高的病例,肺癌细胞凋亡也多,与表达低的病例之间有明显差异,P<0．01。TNF-α表达的高低与间质细胞凋亡之间没有明确的关系。间质细胞平均凋亡指数在高、低分化的肺癌中分别为3．4±0．4和 3．1±0．5,组间没有显著性差异,P>0．015;癌细胞的平均凋亡指数在高、低分化的肺癌中分别为2．4±0．3和 1．7±0．4,两组间有显著性差异,P<0．05,,结论:TNF-α-308位核苷酸的多态性基因型在肺癌和正常人中的分布之间没有明显差别,这种多态性可能不是肺癌易感的遗传因素。但这种多态性与该基因的表达有关。TNF-α高表达肺癌细胞凋亡较多,间质细胞凋亡与TNF-α表达的高低变化之间没有明确的关系。在高分化的肺癌中, TNF-α表达较高,癌细胞凋亡也多,TNF-α-308位核苷酸的多态性可能与了肺癌的分化有关。     

Local Context and Democratization in Mexico

While democratization is often seen as a national-level process, we argue that there is important scope for local effects. Through analysis of Mexican public opinion data collected on the eve of that country's historic 2000 elections, we demonstrate that local context greatly affects evaluations of the legitimacy of the system, and these evaluations, in turn, help to shape the willingness of citizens to engage with the system. Citizens are more willing to participate in politics if they think the process is fair, and direct evidence of the fairness of the system is provided by the local political context. This local connection, then, becomes critical in the process of individuals becoming, in Almond and Verba's classic term, "participatory" citizens (1963).     

Chief executive officers and voluntary environmental performance: Costa Rica's certification for sustainable tourism

This study evaluates whether the education, environmental expertise, and nationality of firms' chief executive officers (CEOs) are associated with greater participation and environmental performance in a voluntary environmental program implemented in a developing nation. Specifically, we collected data from the Certification for Sustainable Tourism (CST) program, a voluntary initiative aimed at promoting beyond-compliance environmental performance by hotels operating in Costa Rica. Our findings suggest that CEOs' level of formal education and environmental expertise appear to be significantly associated with higher corporate participation in voluntary programs and also with higher beyond-compliance environmental performance ratings. Contrary to conventional expectations, CEOs from industrialized countries (as opposed to developing countries) do not appear to show a statistically significant association with participation in the CST program and with higher beyond-compliance environmental performance.     

Recent Developments to British Multicultural Theory,Policy and Practice: The Case of British Muslims

Since the early 1970s, there have been highly sophisticated arguments and conceptual discussions put forward in relation to how Western liberal democracies might wish to manage their diverse ethnic minority populations. It is apparent, however, that in the current climate, the important principles of unity and diversity are insufficient to challenge differing forms of ethnic, racial, and religious inequality. This paper argues that because of its underlying assumptions and modus operandi in the post-September 11 climate, British multiculturalism has been ineffective, even supporting some commentators' suggestions of a return to assimilationism. There is a new era in post-September 11 political hegemony, economic determinism and structural and cultural racisms, with European government rhetoric and public policy almost exclusively aimed at Muslims—whether as existing citizens or as part of a process of limiting immigration (which has tended to be from mainly Eastern European, North African, and Middle Eastern sending regions and nations). The example of New Labour and how it has attempted to deal with multiculturalism in Britain is a case in point. The discussion explores the changing concept of multiculturalism with special reference to British Muslims and debates that emerge in relation to identity, nation and civil society. It is argued that the experience and treatment of British Muslims is important to explore and appreciate in the current climate as it provides a test case for the future of (a) British multiculturalism and (b) British Muslims in society per se.     

注重考试环节提高教学质量

考试是教学的重要环节,不同的学科、不同的课程有不同的特点。命题应严格遵循全面考核,突出重点,理论联系实际,注重考查学生的实务操作能力这一原则。在试题内容上,应难易适度;在阅卷方式上,应根据题型的不同采用不同的判分标准;在应试技巧上,应教导学生要懂、吃透基础知识,学会总结典型题型。同时应加强学生考前模拟训练。     

新时期福建省社会治安综合治理工作的改革与创新

新时期的福建社会治安综合治理工作要在治理理念、运行机制和有效载体上不断创新。在理念上,要正确认清省情,科学分析治安形势,不断创新“平安福建”建设的方略,从更高起点、更高层次、更高要求上来谋划平安建设;在机制上,要构建大综治格局、完善大调解机制、创新大服务模式、健全大防控体系、构筑大基础平台;载体上,有十户（厂、店）联防、中心户长制、“大调解”、治安捆绑问责制等有效载体和流动人口服务管理新模式,以及全方位、多层次的大防控体系。     

自组织理论视角下的人肉搜索现象研究

自组织理论是一种系统理论,它最初针对的研究对象是复杂生命体的结构,后来这一理论的应用范围又扩展至数学和经济学、管理学等领域。将自组织理论引入到传播学研究,分析网络人肉搜索现象是学术探索的一次尝试。自组织理论在传播学中的应用广泛,以“铜须门”事件作为研究案例,分析人肉搜索现象的发生和发展机理,我们能够为处理人肉搜索现象及其类似的网络舆论事件寻找出更好的解决途经。