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991.
Shahtoosh, the down hair of the Tibetan antelope (Pantholops hodgsonii), is the noblest and most expensive wool in the world. The population of the animal has declined dramatically due to commercial poaching for the fiber. Traditional inspection for detection of shahtoosh has been performed by microscopic analysis. We developed a TaqMan real‐time PCR‐based DNA analysis method for identifying shahtoosh fibers. A set of probe and primers for the mitochondrial 12S ribosomal RNA gene that binds specifically to Tibetan antelope DNA was designed. A signal was detected with sensitivity to the 1:10,000 dilution of shahtoosh DNA. A fiber mixture of 1% of shahtoosh mixed with cashmere and even a single fiber can be detected with this method. The method is faster, more cost‐effective and more sensitive than other traditional sequencing methods and can be directly applied to identify shahtoosh and its processed products, which will be of value in illegal trade investigations.  相似文献   
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994.
目的观察分析静脉注射氯胺酮家兔死后血液流动对体内药物浓度再分布影响。方法雄性新西兰大白兔随机分为实验组2组(各24只),对照组(8只);实验组家兔经耳缘静脉注入40mg/kg氯胺酮,1.5h后处死,其中一组立即结扎主动脉,另一组不结扎;家兔尸体仰卧位室温下保存,分别于死后0、3、6、12、24、48、72和96h解剖并采取组织和体液样本;对照组静脉注射等量生理盐水,同样方法解剖取相同样本。所有样本采用GC/MS和GC-NPD法检测样品中氯胺酮含量。结果两个实验组家兔尸体放置96h内氯胺酮含量,除尿液各时间点与0h以及相邻时间点之间比较均有显著性差异(P〈0.05)外,两组家兔其余各类样本与0h以及相邻时间点比较均无显著性差异(P〉0.05)。除尿液外,各类样本两个实验组之间均无显著性差异(P〉0.05);结扎组和不结扎组心血与外周血中氯胺酮含量比值分别为0.90~1.03和0.90~1.02。结论静脉注射氯胺酮家兔死后的血液流动不是体内氯胺酮发生再分布的主要机制。  相似文献   
995.
How do legislators behave in systems where pursuit of re-election is not the rule, and ambition is channelled through multiple levels of government? Is their legislative behaviour biased towards their immediate career goals? In this paper, the Argentine case is analysed in order to explore the link between political ambition and legislative performance in a multilevel setting where politicians have subnational executive positions as priorities, rather than stable legislative careerism. The piece demonstrates that legislators seeking mayoral positions tend to submit more district-level legislation than their peers. This finding contributes to the knowledge of strategic behaviour in multilevel settings, and provides non-US-based evidence regarding the use of non-roll call position-taking devices.  相似文献   
996.
This study explores romantic relationships among female juvenile offenders. The entire female population of a juvenile detention center at the moment of the study participated in a semi-structured interview. Using a semi-structured interview, the girls answered questions on four main topics: (a) available dating partners before entering the detention center; (b) dating partner preferences; (c) intimate relationships within the center; and (d) romantic relationships. A 34-item scale was also used to assess their dating partner preferences. Most girls depicted dysfunctional families and a reduced range of dating options, many involving male offenders. Most participants had also been precocious in their relationships and experienced victimization at the hands of their intimate partners. Implications to prevent female involvement with criminal and violent men are discussed.  相似文献   
997.
It is a maxim of Public Choice that voluntary exchanges should not be interfered with by the state. But what makes a voluntary market exchange truly voluntary? We suggest, contra much of the economics literature, that voluntary exchange requires consent uncoerced by threats of harm, but that this is not sufficient. In particular, a person pressured to exchange by the dire consequences of failing to exchange—e.g., dying of thirst or hunger—is still coerced, and coerced exchange cannot be voluntary. The weaker party’s desperation gives the other party unconscionable bargaining power. We argue for a distinction, based on a neologism: in the case of coercion by circumstance but not by threat, exchange is still voluntary in the conventional sense, but it is not euvoluntary (i.e., truly voluntary). We will argue that all euvoluntary exchanges are just, while non-euvoluntary exchanges may or may not be unjust; that in competitive markets all exchanges are just, even those that are not euvoluntary, while in bilateral monopolies some exchanges are neither euvoluntary nor just. We will propose a mental device, the “fictitious negotiation”, to determine the just price in non-euvoluntary market exchanges. A primitive version of these ideas can be found in a little known monograph by John Locke, which we will analyze in detail.  相似文献   
998.
Albert Heckscher (1857–1897) was a Danish lawyer. In his dissertation (Bidrag till Grundlæggelse af en Afstemningslære), accepted in 1892 at the University of Copenhagen, Heckscher dealt with numerous issues related to voting, especially those related to vote-aggregation in parliaments, courts and committees. He knew the works of Condorcet and Borda quite well, and analyzed many topics that would nowadays fall into the domain of the theory of social choice. These include Condorcet-cycles, differences between Condorcet-effective rules and the Borda rule, strategic voting, the influence of the voting order under the parliamentary voting rules, the likelihood of single-peaked preference profiles, and the problems created by non-separable preferences. Heckscher’s treatment of the Judgment Aggregation Paradox is especially noteworthy. Although Kornhauser and Sager (Yale Law Journal 96: 82–117, 1986) are usually mentioned as the inventors of this problem, Heckscher’s earlier treatment confirms the suspicion that the problem is not of recent origin. Numerous issues studied in the post-Arrowian theory of voting may already be found in Heckscher’s dissertation; some of them have become subjects of systematic study only in the twenty-first century. It is argued that Albert Heckscher, the unknown nineteenth century Danish lawyer, deserves a place in the pantheon of the theory of social choice alongside his better known contemporaries Charles Dodgson and E. J. Nanson.  相似文献   
999.
This is not an academic overview on public affairs in Hungary, as this has already been written very well by many others. I would like to share my views and experience as a practitioner and give a quick insight into how public affairs is regarded in my country and how it developed after the change of the political system in Central and Eastern Europe. I will refrain from explaining what public relations or public affairs entails as I think this is unnecessary for anyone reading this. Copyright © 2014 John Wiley & Sons, Ltd.  相似文献   
1000.
Immunohistochemical detection of molecules involved in inflammatory reaction can be useful for the diagnosis of vitality in skin wounds. We studied the expression of fibronectin (FN) and tenascin (TN) in 58 human skin wounds (48 vital and 10 postmortem). The age of vital injuries ranged from 3 min to 8 h and postmortem specimens were collected after a postinfliction interval of 15-180 min. One hundred thirty-seven formalin-fixed paraffin-embedded sections (mean: 2.3 sections per case) were stained with each of two monoclonal antibodies against FN and TN using the streptABC technique. A reticular staining for FN in wound edge and dermis was observed in 50% of vital specimens versus 0% in postmortem cases. Immunoreactivity was reduced in 10 autolysed cases. FN positivity exclusively at the injury margin was observed in 39.4% of vital wounds and 10% of postmortem cases. TN was negative in all specimens. Vital and postmortem hemorrhage areas showed positivity for FN and TN. Due to its low sensitivity, immunohistochemical analysis of FN is useful for determining vitality only in a minority of cases. Different factors in everyday practice, including autolysis and technical problems often produce false negative reactions with the result that FN cannot be regarded as a reliable parameter of vitality. Positive reactions (network staining) are more valuable than negativity but are not pathognomonic. Both vital and postmortem hemorrhages show an enhanced positivity for FN and TN, thus impeding the diagnosis.  相似文献   
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