蓝舌病病毒NS2蛋白单克隆抗体的制备及其应用

本研究旨在制备针对蓝舌病病毒(bluetongue virus,BTV)非结构蛋白NS2的单克隆抗体,进而用于C-ELISA鉴别BTV自然感染动物与BTV灭活疫苗免疫动物。通过原核表达BTV NS2蛋白的高保守多肽(1~228 aa)来制备抗原,并通过免疫BALB/c小鼠制备抗BTV NS2蛋白的单克隆抗体,结果,获得1株分泌单克隆抗体的小鼠杂交瘤细胞,命名为BTV-2A4。鉴定结果显示,该单克隆抗体的亚类/亚型为IgG1/κ;其抗原表位在NS2蛋白的91~138 aa区间;能特异性地与1~24型BTV的NS2蛋白反应;可应用于Western-blot、间接免疫荧光、间接ELISA及C-ELISA技术。C-ELISA应用的试验数据表明,BTV疫苗免疫动物及未被BTV感染动物的血清中检测不到NS2抗体,而BTV感染动物的血清中大多可被检出NS2抗体。     

Thoughts on China＇ s Diplomacy in a Changing World

The world has seen great changes over the past two decades:the emergence of new political forces,proliferating challenges and threats,and louder voices from new opinion agents.China’s foreign diplomacy is confronted with difficult issues in seeking to ensure a peaceful external environment to preserve its domestic stability and boost its modernization drive.Efforts need to be made to harmonize relationships in six key areas.     

试析康德关于道德情感的理论

从道德情感的本质结构和作用机制,可将康德的道德哲学定位为形式主义与义务论的传统观念;康德对道德情感的态度是全面而适当的,长久以来人们对康德的关于道德情感的观点的批评建立在对他的误解之上;其实,康德并未忽视人类道德情感的复杂性与丰富性;从康德的批判哲学的视野出发,道德情感得到了全面的考虑,并获得了恰当的价值与地位。     

Domestic Predation of an Elder: A Fatal Dog Attack Case

We present the case of a 91‐year‐old woman lived alone at her home with two domestic dogs, that is,, a Labrador Retriever and a Staffordshire Bull Terrier and found dead. The investigation of the scene revealed that the Bull Terrier's jawbone and chest were covered with blood. The autopsy revealed multiple, histologically confirmed, life‐threatening skin and bone lacerations without scavenging marks. The punctures and tearing of each of the wounds on the skin were compatible with bites. A left humeral fracture and multiple fractures of the right facial bones were observed. The death was attributed to external hemorrhages due to several dog bites. A veterinary physical and behavioral examination indicated that the Bull Terrier was involved in the attack. A domestic predation hypothesis was deemed here most likely due to the presence of food supplies at the scene, the dog's previous history of attack, and the breed of the dog.     

Offender personality disorder pathway: the impact of case consultation and formulation with probation staff

The latest government policies for personality disordered offenders emphasise the importance of a formulation-based approach to rehabilitation and pathway planning. However, research into forensic case formulation is limited. This paper examines the impact of consultation and formulation, on probation staff working with personality disordered offenders. Staff rated their knowledge, confidence, motivation, and understanding (of offenders), and satisfaction with management plans at three stages: pre-consultation, post-consultation and after receiving a written formulation. The analyses revealed that ratings on all variables examined increased after attending a consultation meeting, with no additional increase in scores following receipt of the formulation letter. The findings provide evidence that consultation has a range of benefits for probation staff, and offer support for the current model of joint-agency working. Further research is needed to clarify the benefits of providing written formulations after consultations, and to explore what features of a formulation are most useful to probation staff.     

中国知识型服务贸易的国际竞争力测算

知识型服务贸易的国际竞争力体现了一国知识经济的发展程度.通过使用RCA和NXRCA指数,对中国在内的九个国家2002年和2005年知识型服务贸易国际竞争力进行测算,可以看出,欧美发达国家在知识型服务贸易上具有明显的比较优势,而中国知识型服务贸易的国际竞争力相对较弱.大力发展知识经济,推进创新型国家建设是提升我国知识型服务贸易国际竞争力的必由之路.     

司法如何面对道德?

@@ 一、司法何以如此尴尬?20世纪90年代后期以来,针对社会急剧转型以及由此所带来的纠纷的重大变化,中国的司法改革作了很多尝试.可以说,这些尝试对当代中国法院制度的变革所起到的作用是全方位的.     

Psychosocial and Symbolic Dimensions of the Breast Explored through a Visual Matrix

This article explores knowledge about the breast in lived experience, addressing a gap in empirical research on a highly gendered cultural trope and embodied organ. We present findings from a study that used a free-associative psychosocial method—the Visual Matrix—in order to stimulate expressions of tacit aspects of the breast, aiming to generate an understanding of relations between embodied and enculturated experiences. Our data revealed how an aesthetic of the grotesque in one matrix allowed the mainly female group to use humour as a “creative psychic defence” against culturally normative and idealized aspects of the breast. This was expressed through symbolizations, affectively delivered in an exuberant mode, emphasizing the breast‘s potency and its potential for nurturance and “weaponization”. Through this feminine poetic, life and death became inseparable yet ambiguous dimensions of breasts. The breast’s life-affirming qualities included the sensual, the visceral, and the joyful—a material-semiotic knowing. This was incontrast to a second matrix, which expressed a more ambivalent and troubled response, and in which associations were weighted towards the spectacular breast of an ocular-centric culture that privileges hetero-masculine looking. We discuss differences between the two matrices in terms of psychosocial tensions between embodied and enculturated experiences.