Isolated rat cardiac myocytes as an experimental tool in the study of anoxic cell injury. Effect of reoxygenation — A preliminary report

Rat cardiac myocytes were isolated by heart perfusion in the presence of collagenase and incubated in the absence or presence of oxygen. As a result of anoxia, there was a gradual increase in plasma membrane permeability, noted as a decrease in trypan blue exclusion frequency, leakage of cytosolic lactate dehydrogenase and intracellular accumulation of the isotope compound ⁹⁹Tc^m-gluconate. The changes in plasma membrane permeability properties were preceded by a marked decrease in cellular ATP level and an increased proportion of contracted myocytes. The ability of the myocytes to resynthesize ATP and to recover from the anoxic injury upon reoxygenation decreased gradually with the length of initial anaerobic incubation during the first 25 min and disappeared after 30 min of anoxia, indicating that the anoxic injury to the isolated rat cardiac myocytes becomes irreversible after 25 – 30 min of anoxia. It is suggested that a decreased energy level is of primary importance for the initiation of cell injury in anoxia and that it is followed by cell contracture and subsequently by a disturbed plasma membrane function, cell swelling and death. This experimental model system of isolated viable rat cardiac myocytes is suitable for problems dealing with reversibility of myocytic injury.     

Intra-familial child homicide in Finland 1970-1994: incidence, causes of death and demographic characteristics

The purpose of this study was to investigate all child homicides for the 25-year period, 1970-1994 in Finland and to analyse the specific characteristics of the filicide cases. A total of 292 child homicides occurred during this period. In 201 (69%) cases the offender was a parent or a stepparent of the child. Altogether, 173 (59%) of the victims were boys and 119 (41%) were girls. For the closer examination of the filicide cases we excluded the neonaticide and homicide-suicide cases. Consequently, we report on 70 filicide victims. Of these victims, 42 (60%) were boys and 28 (40%) were girls. Twenty six (37%) of the children were killed before the age of 1 year and 53 (79%) before the age of 5 years. The offender was the mother in 43 (61%) cases and the father or the stepfather in 26 (37%) cases. The victims of the mothers were younger than those of the fathers. The most frequent causes of death were head injuries, drowning and suffocation. The most common means of assault were battering, drowning and strangulation. One in two of the fatally battered children had a documented history of previous abuse.     

Simultaneous screening and quantitation of 18 antihistamine drugs in blood by liquid chromatography ionspray tandem mass spectrometry

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is presented for the simultaneous screening and quantitation of 18 antihistamine drugs in blood samples. Sample pretreatment involved liquid-liquid extraction of the basic antihistamines followed by a second extraction of the acidic antihistamines. The recoveries were 43-113% for basic drugs and 23-66% for acidic drugs. The combined extracts were run by LC on C(18) reversed phase column using acetonitrile-ammonium acetate mobile phase at pH 3.2. The mass spectrometric analysis was performed with a triple stage quadrupole mass analyzer. Screening was performed using multiple reaction monitoring (MRM) and any compounds tentatively identified as antihistamine drugs were then automatedly verified by their Product Ion Spectra in a subsequent MS/MS run.Quantitation was based on the MRM data from the screening step. In validation tests, the method showed good linearity at the relevant concentrations. The attained limits of quantitation varied between 0.0005 and 0.01mg/l in blood and were lower than the therapeutic concentrations (C(max)). The limits for identification by Product Ion Spectra were also lower than C(max), except for clemastine, which has exceptionally low concentrations in blood. The intra-assay relative standard deviations were better than 10% and the inaccuracy varied between 39% for levocabastine and 5% for cyclizine, the majority of the values being <20%.     

Roadside oral fluid testing: comparison of the results of drugwipe 5 and drugwipe benzodiazepines on-site tests with laboratory confirmation results of oral fluid and whole blood

Drugged drivers pose a serious threat to other people in traffic as well as to themselves. Reliable oral fluid screening devices for on-site screening of drugged drivers would be both a useful and convenient means for traffic control. In this study we evaluated the appropriateness of Drugwipe 5 and Drugwipe Benzodiazepines oral fluid on-site tests for roadside drug screening. Drivers suspected of driving under the influence of drugs were screened with the Drugwipe tests. Oral fluid and whole blood samples were collected from the drivers and tested for amphetamine-type stimulant drugs, cannabis, opiates, cocaine and benzodiazepines by immunological methods, GC and GC-MS. The performance evaluations of the tests were made by comparing the results of the Drugwipe tests with laboratory GC-MS confirmation results of oral fluid or whole blood. In addition to the performance evaluations of the Drugwipe tests based on laboratory results, a questionnaire on the practical aspects of the tests was written for the police officers who performed the tests. The aim of the questionnaire was to obtain user comments on the practicality of the tests as well as the advantages and weak points of the tests. The results of the performance evaluations were: for oral fluid (sensitivity; specificity; accuracy) amphetamines (95.5%; 92.9%; 95.3%), cannabis (52.2%; 91.2%; 85.1%), cocaine (50.0%; 99.3%; 98.6%), opiates (100%; 95.8%; 95.9%), benzodiazepines (74.4%; 84.2%; 79.2%) and for whole blood accordingly, amphetamines (97.7%; 86.7%; 95.9%), cannabis (68.3%; 87.9%; 84.9%), cocaine (50.0%; 98.5%; 97.7%), opiates (87.5%; 96.9%; 96.6%) and benzodiazepines (66.7%; 87.0%; 74.4%). Although the Drugwipe 5 successfully detected amphetamine-type stimulant drugs and the police officers were quite pleased with the current features of the Drugwipe tests, improvements must still be made regarding the detection of cannabis and benzodiazepines.     

Comparative Perspectives on Childcare Expansion in Germany: Explaining the Persistent East‒West Divide

Abstract

Germany’s family policy responded to the European Union’s Barcelona targets by expanding publicly funded childcare, especially for children under the age of three, throughout the country. While overall nearly 33 per cent of all children in that age group had access to public childcare by 2015, there is still a large difference between the situation in the eastern and the western part of the country. This paper documents and explains this development of two persistently different childcare regimes in the two parts of the country. It shows that supply factors such as local administrative capacity and resources interact with demand factors such as parental need and demands for public childcare to produce the divergent development. It then discusses the conceptual challenges involved in engaging in this type of subnationally comparative analysis.     

Cleavages – Dead and Gone? An Analysis of Cleavage Structure and Party Choice in Contemporary Finland

In cleavage research in political science, few studies have tested the applicability of a threefold cleavage strategy when analysing national cleavage structures. This study, which has contemporary Finland as a test case, makes two contributions in evaluating the continued relevance of cleavage research. First, it assesses both old and new social structural positions simultaneously in order to explore how they are reflected in the values and attitudes in the electorate. Second, it analyses how these cleavage elements are manifested in party choice and discovers whether value/attitudinal dimensions act as intermediate variables between social structural position and the vote. The statistical analyses are conducted with data from the Finnish National Election Studies from 2003–11. The results show that threefold cleavage definition is not satisfactory for identifying the dynamics in the party‐voter‐ties despite Finland often being regarded as the epitome of a cleavage‐based system. The effect of social structural position on value/attitudinal dimensions is fairly weak and the effect of the social structural position on party choice is mediated through values and attitudes only in some cases. Even though the remnants of cleavage politics remain in Finland, with conflicts based on native language and type of residential area best fulfilling the cleavage criteria, the threefold cleavage does not serve to describe the whole complex set of political conflicts. Since Finland has had an archetypical status as a system of cleavage‐based party support, further elaborations on cleavages are needed to understand the complexity of present conflict structures in any established Western democracy.     

Large‐scale solidarity? Effects of welfare state institutions on the admission of forced migrants

The variation among countries when it comes to the admittance of forced migrants – refugees and asylum seekers – is substantial. This article explains part of this variation by developing and testing an institutional explanation to the admission of forced migrants; more precisely, it investigates the impact of domestic welfare state institutions on admission. Building on comparative welfare state research, it is hypothesised that comprehensive welfare state institutions will have a positive effect on the admission of forced migrants to a country. There are three features of comprehensive welfare state institutions that could steer policies towards forced migrants in a more open direction. First, these institutions have been shown to impact on the boundaries of social solidarity. Second, they enhance generalised trust. And third, they can impact on the citizens’ view of what the state should and can do in terms of protecting individuals. The argument is tested using a broad comparative dataset of patterns of forced migration, covering 17 OECD countries between 1980 and 2003. This analysis shows that comprehensive welfare state institutions have a significant positive effect on the admission of forced migrants, under control for a number of factors often highlighted in migration research.