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271.
Kelly A. Meiklejohn B.Biotech. James F. Wallman Ph.D. Mark Dowton Ph.D. 《Journal of forensic sciences》2013,58(1):184-187
Carrion‐breeding insects, such as flesh flies (Diptera: Sarcophagidae), can be used as evidence in forensic investigations. Despite their considerable forensic potential, their use has been limited because morphological species identification, at any life stage, is very challenging. This study investigated whether DNA could be extracted and cytochrome oxidase subunit I (COI) barcode sequences obtained for molecular identification of each immature life stage of the forensically important Australian flesh fly, Sarcophaga (Sarcorohdendorfia) impatiens (Walker). Genomic DNA extracts were prepared from all larval instars and puparia. Amplification of the barcoding region was successful from all extracts, but puparia amplicons were weak. All sequences were identified as S. impatiens with 99.95% confidence using the Barcoding of Life Database (BOLD). Importantly, crop removal was necessary to eliminate PCR inhibition for specimens from late second and early third instars. Similar results are expected for immatures of other carrion‐breeding species, enhancing the use of evidence from immature flies in forensic investigations. 相似文献
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Ryan Deschamps Kathleen McNutt 《Canadian public administration. Administration publique du Canada》2016,59(1):45-71
Cyberbullying has been a difficult problem for policy makers and observers to define. For some, cyberbullying is understood as a public health problem, while others view it largely as an education issue, and still others see it as a justice problem. In Canada, while the definition of cyberbullying continues to evolve, a nascent approach assumes that it is similar to traditional face‐to‐face bullying with computer‐mediated communication as a new element. This definition is at odds with recent research on cyberbullying, which may have significant implications for policy makers seeking to design effective interventions. 相似文献
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Jose G. Lavoie Joseph Kaufert Annette J. Browne Sharon Mah John D. O'Neil Stephanie Sinclair Kathleen BlueSky 《Canadian public administration. Administration publique du Canada》2015,58(2):295-314
This study documents the policy framework that shapes First Nations' experience of relocating to access medical care. We analyzed policy documents and government websites, interviewed individuals who have experienced relocation, family members, healthcare providers, health administrators, and conducted two focus groups with government representatives. Federal and provincial program managers interpret policies, make decisions on eligibility, to extend or deny coverage. Decisions are shaped by shrinking budgets, fragmented program coverage, and jurisdictional confusion. Provider advocacy can help, but also perpetuate perceptions of arbitrariness and distrust. National policy renewal is required to redress this issue. 相似文献
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Roche Kathleen M. Huebner David M. Lambert Sharon F. Little Todd D. 《Journal of youth and adolescence》2022,51(6):1031-1047
Journal of Youth and Adolescence - This study addressed the need for research examining impacts of the Coronavirus-19 (COVID) pandemic on Latinx adolescents’ adjustment. Survey data for a... 相似文献