Recovery of genomic DNA from archived PCR product mixes for subsequent multiplex amplification and typing of additional loci: forensic significance for older unsolved criminal cases

A method for genomic DNA recovery from different types of PCR product mixes suitable for multiplex amplification and typing using the Profiler Plus STR typing system has been investigated. The application of this method is of significance in cases where the original DNA samples have been exhausted due to repeated typing analyses in an effort to maximize their evidentiary value. Such cases typically involve samples analyzed using the available DNA typing systems of the time which gave a markedly lower power of discrimination, either alone or in combination, compared to that of modern multiplex STR typing systems. It was found that an effective method for recovering genomic DNA from HLA-DQA1 +PM and CTT triplex amplification mixes, suitable for reproducible achievement of the complete Profiler Plus profile, involved the use of Amicon Microcon-100 microconcentrators. Interestingly, this method was not required to achieve the complete nine STR profile using D1S80 amplification mixes.     

Posthumous reproduction: a consideration of the medical, ethical, cultural, psychosocial and legal perspectives in the New Zealand context

This paper describes the development of New Zealand policy on posthumous reproduction in assisted human reproduction. It outlines five perspectives: medical, ethical, cultural, psychosocial and legal and shows the multidisciplinary approach taken by the National Ethics Committee. It is argued that each of these perspectives has important contributions to make to the multidisciplinary approach. The guidelines determined by the Committee are outlined, along with the processes used in arriving at these.     

Japan’s search for influence in the Korean peninsula after the Cold War: Aspirations and constraints

This article examines Japan’s search for a more active international role in the Korean peninsula after the Cold War. It argues that Japanese foreign policy toward the Korean peninsula after the Cold War has continued to exhibit a reactive posture due to several important constraints. The United States has remained an important factor in shaping the Japanese foreign policy agenda in the post-Cold War era. Moreover, the Japanese domestic political environment, despite the dramatic political realignment after 1993, has continued to favor a nonmilitary Japanese role in world affairs. Finally, the lingering Korean mistrust toward Japan has hampered Japan’s bid to increase its political profile. Consequently, these constraints ensure that Japan’s independent role in the Korean peninsula will be rather limited in the foreseeable future.     

Surveying Research on Parliament and Parliamentary Oversight of the Public Sector

This presentation begins by offering a brief overview of the existing research into Parliament and Parliamentary Oversight of the Public Sector. It highlights the work of those who are experts in the area and concludes by identifying areas which would benefit from further research.     

The effectiveness of the telecommunications regulatory regime: the case of Sri Lanka telecom

This article examines the telecommunications industry in Sri Lanka and assesses the effectiveness of regulatory arrangements associated with the liberalisation of the telecommunications industry, from a management point of view. The review focuses on the scope of services, price and the quality of services available to customers after the liberalisation. This study finds that, despite the early establishment of the Telecommunications Regulatory Commission (TRC) to monitor the telecommunications industry, its interventions have been only partially successful in making it conducive to service providers and customers. While liberalisation of the telecommunications industry has been favoured, the role of the regulator has been controversial with regard to its independence, impartiality, capability, transparency and accountability. We argue that the current model has failed to create favourable market conditions under the circumstances prevailing in the country, and hence a more appropriate model is yet to be developed. Copyright © 2006 John Wiley & Sons, Ltd.     

Mortality surveillance: 2004 to 2005 Florida hurricane-related deaths

During 2004 and 2005, Florida was struck by 8 hurricanes, resulting in 213 deaths. The Department of Health and Florida medical examiners monitor hurricane mortality surveillance. This study analyzed hurricane-related deaths reported by the Florida Medical Examiners Commission for 2004 to 2005. The objectives of this study were to (1) describe the Florida hurricane-related mortality for 2004 and 2005, (2) accurately characterize the hurricane-related deaths, and (3) identify strategies to prevent or reduce future hurricane deaths. For 2004, there were 144 total hurricane-related deaths. The majority (59%) occurred in the postimpact phase, with accidents accounting for 76% of deaths. Among these, over half were caused by trauma, followed by drowning, other injury, electrocution, and carbon monoxide poisoning. For 2005, there were 69 hurricane-related deaths. Sixty-one percent of deaths occurred in the postimpact phase, with accidents accounting for 86% of all deaths. Among these, over half were due to trauma, with drowning and carbon monoxide poisoning being the other major contributors. Most hurricane-related deaths are due to unintentional injury and therefore, preventable. Seventy-nine percent of deaths are in those aged 40 and older. Prevention messages should target high-risk, postimpact activities, especially in older adults.