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ABO genotyping is often performed to identify the blood type of decomposed samples, which is difficult to be determined by a serological test. In this study, we developed a simple method for ABO genotyping using a DNA chip. In this method, polymerase chain reaction-amplified and fluorescent-labeled fragments in the ABO gene and primate-specific D17Z1 were hybridized with DNA probes on a chip designed to detect single nucleotide polymorphisms (SNPs) in the ABO gene and part of the D17Z1 sequence. Using blood samples from 42 volunteers and 10 animal species, we investigated whether the chip could be used to detect SNPs in the ABO gene and the D17Z1 sequence. This method was then applied to various forensic samples, and it was confirmed that this method was suitable for the simultaneous analyses of ABO genotyping and species identification. This method fulfills the recent need for the development of rapid and convenient methods for criminal investigations.  相似文献   
164.
Ken Yoshida 《Japan Forum》2014,26(4):486-507
This article examines art criticism of the 1980s that sought to address the context and continuity of postwar Japanese avant-garde art. The emphasis is placed on the notion of ‘the genus of art [rui to shite no bijutsu]’, which the art critic Chiba Shigeo discussed in his Gendai bijutsu itsudatsushi (1986). Chiba argued for an autonomous current of postwar Japanese art separate from Western art history but the argument has been largely overshadowed by his ostensible nihonjinron. The term ‘the genus of art’, therefore, has not yet received an adequate critical treatment. The article rephrases this notion as a call for a more inclusive definition of art designed to challenge the conditions that regulate non-Western art. Rather than simply being a nationalist polemic against Western art history from a marginalized place, Japan, ‘the genus of art’ was intended to revitalize and redefine the aims of the avant-garde within the context of ‘contemporary art’. By fleshing out the textual details of ‘the genus of art’ and situating the notion within a larger discourse that interrogated the semiotic role of ‘Japan’ in a global exhibition context, the study repositions Chiba's writing as a potential argument for unconditional acceptance, or unconditionality, as one important ethical contribution of contemporary art.  相似文献   
165.
Securing public funding to conduct research and leading it by being a principal investigator (PI) is seen as significant career development step. Such a role brings professional prestige but also new responsibilities beyond research leadership to research management. If public funding brings financial and infrastructure support, little is understood about the inhibiting factors that publicly funded PIs face given the research autonomy offered by publicly funded research. Our study finds that there are three key PI inhibiting factors (1) political and environmental, (2) institutional and (3) project based. Traditional knowledge, skills and technical know-how of publicly funded PIs are insufficient to deal with the increasing managerial demands and expectations i.e. growing external bureaucracy of public funding agencies. Public funding is no longer the ‘freest form of support’ as suggested by Chubin and Hackett (Peerless science: peer review and US science policy. Suny Press, New York, 1990) and the inhibiting factors experienced by publicly funded PIs limits their research autonomy. We also argue that PIs have little influence in overcoming these inhibiting factors despite their central role in conducting publicly funded research.  相似文献   
166.
Using agriculture input sector as an exemplar, this article assesses Bangladesh's efforts towards a market-oriented development approach. It examines the changing role of the state following the market-based reforms undertaken in this sector and assesses whether the outcomes of the reforms are sustainable. Findings reveal that, although the current move towards a market-oriented approach has led to a shift away from a state-dominated hierarchical structure, it has not been associated with adequate changes in institutional arrangements, safeguards, and regulation. As a result, despite notable achievements of the reforms, the sustainability of these successes is in question. To facilitate participatory, accountable, and sustainable, market-oriented development, the paper proposes an integrated governance model linking state, business, and civil society.  相似文献   
167.
Resource constraints, citizen activism, and the threat of privatization are driving public organizations toward responsiveness and efficiency. Hierarchy is giving way to polyarchy in the administrative system. An interactive classroom which uses analysis and feedback to leverage the students’ experiences in management situations can prepare public administration students for this new polyarchal work environment.  相似文献   
168.
This paper provides exploratory insights into the impact of the UK government's Best Value policy on employment policies in voluntary organisations. Best Value is one of a number of institutional pressures on HR policies within the sector: many of which have positive implications for the conduct of HR and delivering care. However, the data also reveal tensions in this institutional environment with regard to meeting demands of twenty-four hour care and family-friendly policies for the workforce. Moreover, continuation of aspects of the New Public Management agenda within Best Value means that pressure on pay and conditions in the sector persist, raising concerns regarding worker morale and quality of care.  相似文献   
169.
The advance of globalisation depends in fundamental ways on the continued flows of transnational labour it has stimulated. For semi-skilled and unskilled service workers these flows are managed through numerous controls imposed by states, employers, labour brokers and even by the expectations of their families and communities of origin. This article concentrates on female service labour originating in Indonesia and the Philippines, destined for Hong Kong and Taiwan. It emphasises the collective (family and community-based) nature of the worker's entry into this type of work. It looks at multiple intersecting discourses that shape, construct and attempt to manage the worker's behaviour at different stages of the migration cycle. It gives particular attention to the receiving workplace: the household, and in some instances, the household-based enterprise. The article shows that workers placed in these households are simultaneously governed by contractual relationships and by historically and culturally derived rules reflecting the household's functions as a property-holding, administrative and patriarchal kinship unit. Globalisation has brought far-reaching changes to patterns of labour migration, but the household as a workplace retains important vestiges of these older patriarchal discourses, and ambiguities remain about the emergence of free labour in domestic work.  相似文献   
170.
Abstract

The current transformation of public and assisted housing reflects the legacy of the Gautreaux case, which created the first mobility and scattered‐site programs. Mixed‐income and dispersal strategies now dominate federal housing policy, although their focus has shifted. Drawing on evidence from two preliminary studies of public housing transformation in Chicago, we argue that these new strategies seem to offer benefits for distressed public housing communities but also involve risks for the most vulnerable current tenants. Increased screening and/or the need to compete with private market tenants may force these families out of the assisted housing market.

Addressing the complex needs of the most troubled public housing tenants will call for a more comprehensive solution. The intent of the Gautreaux case was to increase opportunity and enhance quality of life for public housing tenants; policy makers should take steps to ensure that current programs reflect these fundamental goals.  相似文献   
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