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Development of a Real‐Time PCR‐Based Method for Analyzing Semen‐Specific Unmethylated DNA Regions and Methylation Status in Aged Body Fluid Stains 下载免费PDF全文
Ken Watanabe Ph.D. Tomoko Akutsu Ph.D. Koichi Sakurada D.D.S. Ph.D. 《Journal of forensic sciences》2016,61(Z1):S208-S212
The detection of semen in forensic investigation is considered important evidence of sexual assault. In this study, we report the development of a real‐time polymerase chain reaction‐based method for identifying semen that can simply and rapidly analyze the semen‐specific unmethylated region of the DACT1 gene. Using two fluorescent probes designed for the methylated or unmethylated status, this method could perform quantitative analysis of the methylation status in this region. Furthermore, this method was used to analyze various body fluid samples, including 29‐year‐old semen and blood stains. The results showed that this method can detect almost exclusively semen or nonsemen signals even in highly decomposed samples, while a few semen or nonsemen samples showed slight signals of the other fluorescence probe. Although there is still a need for further analysis such as setting thresholds to analyze unknown samples, this method could be a useful supplementary tool for identifying semen, especially in old stains such as those in cold‐case investigations. 相似文献
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Ken Shimizu 《Asia-Pacific Review》2018,25(1):112-123
The year 2018 marks the 150th anniversary of the establishment of diplomatic relations between Japan and Sweden. It is believed that the first contact between Sweden and Japan came during the Edo Period and was mediated by the Netherlands. The Treaty of Friendship, Commerce and Navigation between Japan and Sweden, signed in 1868, also came into being thanks to the good offices of the Dutch. This treaty, like many others into which Japan entered at that time, was an “unequal treaty.” As Japan increased its presence in East Asia, this unequal treaty with Sweden was revised in 1911. The world was changing. Sweden and Japan shared a common interest in opposing Russia’s ambitions. Russia in particular was a common threat to the diplomatic posture and security of both Japan and Sweden. These two nations, positioned like bookends on either side of Russia, could be important strategic partners for one another and allies in their relationships with Russia. Considering the current issue of North Korea, Sweden also has the potential to act as a mediator because it maintains diplomatic relations with North Korea. There is reason to believe that the diplomatic relationship between Japan and Sweden will develop further. 相似文献
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Ken Young 《Intelligence & National Security》2013,28(6):901-925
When Socialist intellectual John Strachey was appointed as Secretary of State for War in 1950, his pre-war record as a Marxist writer with close connections to the Communist Party of Great Britain (CPGB) became a matter of public debate. A bitter campaign was run against him in the Beaverbrook press, and some members of the US defense and nuclear establishment pressed for an embargo on sensitive information being passed to the UK War Office. American suspicion of the political reliability of the Labour government was heightened by the appointment, but this does not explain how and why some Americans were so hostile to Strachey. The FBI's dossier on his pre-war activities, circulated amongst his American critics, documented Strachey's supposed secret membership of the CPGB's Central Committee. MI5 and Special Branch files show that this supposition was based on faulty intelligence. The readiness of American anti-Soviet protagonists to lend credence to such suspicions contrasts with the relaxed view of Strachey's past that was taken in Whitehall. Both positions were characteristic of their time, and of this stage in the Anglo-American alliance. This paper explores the ways in which American insecurities and a British climate of tolerance towards fellow travellers shaped the way that episode played out. 相似文献