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81.
Preparation of degraded human DNA under controlled conditions 总被引:7,自引:0,他引:7
DNA typing through analysis of short tandem repeats (STRs) and mitochondrial DNA (mtDNA) by means of the polymerase chain reaction (PCR) and sequencing are the common methods for the forensic identification of persons and reconstruction of kinship, especially when skeletal human remains have to be analyzed. Furthermore, samples typically found at crime scenes may be both quantitatively and qualitatively inadequate since they may contain very scarce and often degraded DNA due to exposure to heat, light, humidity, and microorganisms. In order to improve the performance of STR typing technology in those cases where DNA availability is limited, it would be desirable to have a source of degraded DNA with known properties. For this purpose, we have developed a method to prepare artificially degraded DNA under controlled conditions. By treatment of genomic DNA with sonication and DNAse I we have produced DNA fragments within a defined range of lengths. STR typing of this degraded DNA with a commercially available multiplex kit could only produce partial profiles as indicated by the absence of STR alleles with sizes >200 bp. This artificially degraded DNA can be used for the improvement and standardization of STR typing protocols when only highly degraded DNA is available for analysis. 相似文献
82.
How has Denmark been able to adjust its economic and industrial policies in an era of major changes in the international economy? The article gives a survey of the last 10 years, especially more recent years. Before 1986 wage policy and public-expenditure policy were dominant. Since then they have been supplemented by a new structural policy to increase the level of technology, to improve education and research and to change the composition of Danish exports as far as products and markets are concerned. The adjustment of policies has taken place in a stable multi-centred political system. The process has been characterized by institutionalized campaigning, moulding of interests and mobilization of compromises and mutual understanding. The capacity for flexible adjustment seems to originate from the stability of the political system. Likewise, uncertainty and instability seem to weaken the capacity for political flexibility. During the last two years the transformation of the elite consensus into state programmes and administrative decision-making has been hampered and the whole structural policy approach has been challenged by a neoliberal alternative. This is mainly due to an extraordinary parliamentary stalemate in a situation of extreme minority parliamentarism. 相似文献
83.
Klaus Eder 《Berliner Journal für Soziologie》2006,16(4):425-428
84.
Macroeconomic Development and Civil Litigation 总被引:1,自引:0,他引:1
This paper contributes to the explanation of the tremendous rise in civil litigation activity in Austria over the last four decades by analysing the influence of macroeconomic conditions on civil litigation. We find that the number of transactions per individual—proxied by the level of real per capita GDP—positively influences the amount of litigation per capita. Inertia in litigious behavior reinforces effects. In the short run, however, we observe a countercyclical pattern of litigious activity. Cointegration analysis confirms the short run negative association, and a long run cointegrating relationship between GDP and civil litigation. Several robustness tests corroborate our results. Rent-seeking interpretations, and possible detrimental effects on the long run growth prospects cannot be excluded. 相似文献
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The human brain shows a very high density of functionally important structures. Even small lesions may therefore cause clinical symptoms. Penetrating gunshots to the head are presumed to cause immediate incapacitation by subsequent disturbance of cerebral functions. The authors discuss anatomical and functional principles of the terms action, capacity to act and incapacitation and report two illustrative cases with lack of incapacitation following gunshots to the head. 相似文献
89.
Klaus von Lampe 《Trends in Organized Crime》2011,14(2-3):278-280
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