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Shanner L 《Health law review》2002,11(1):62-66
Stem cell policy discussions frequently confuse embryonic and fetal sources of stem cells, and label untested, non-reproductive cloning as "therapeutic." Such misnomers distract attention from significant practical and ethical implications: accelerated research agendas tend to be supported at the expense of physical risks to women, theological implications in a multi-faith community, informed consent for participation in research, and treatment decisions altered by unrealistic expectations. 相似文献
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One of the most important longstanding problems in the field of forensic medicine is the determination of the time of death upon the discovery of a possible homicide victim. With a majority of homicide victims discovered within the first 48h, it is critically important to be able to determine time of death quickly, and with accuracy and precision. Current methods of determining postmortem interval (PMI) vary, but none can provide better than an 8-h window time estimate. In this paper, the potential application of single-cell gel electrophoresis (SCGE), also known as the comet assay, to evaluate postmortem cell death processes, specifically nuclear DNA fragmentation, is assessed. Upon the death of an organism, internal nucleases contained within the cells should cause chromosomal DNA to degrade into increasingly smaller fragments over time, and if these fragments can be isolated and visualized, the fragmentation should prove to be measurable and quantifiable. An original study providing proof of the concept of postmortem DNA fragmentation between early and late time periods was conducted using human leukocytes. With an established trend seen in the leukocyte results, this study was then expanded using a porcine animal model, over a longer time period, with more frequent time-points evaluated. DNA degradation in all samples was revealed by SCGE and quantified by the use of DNA-specific quantitative stains, as measured by digital camera affixed to a microscope. The comet 'tail-moment' gave a measure of the proportion of fragmented to non-fragmented DNA, while the 'tail-length' provided the relative size of degraded DNA fragments. In both models, an increase in DNA fragmentation was found to correlate with an increased PMI from 0 to 56h postmortem, as evaluated by comet-tail-moment and by comet-tail-length, with tail-length providing the strongest statistical correlation, based upon regression analysis. The postmortem DNA fragmentation observed in this study, reveals a sequential, time-dependent process with the potential for use as a predictor of PMI in homicide cases. 相似文献
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Manuel L Retzlaff PD 《International journal of offender therapy and comparative criminology》2002,46(5):522-531
Tattooing has always been associated with criminals. Indeed, it is both an administrative and clinical problem in prisons. This article looks at the relationship between psychopathology and tattooing infractions within prison. Using a sample of 8,574 male inmates in the Colorado Department of Corrections, initial admission personality testing was compared to tattooing infractions within the next 2 years. Personality types more likely to engage in tattooing included Antisocial, Sadistic, Negativistic, and Borderline. Compulsive personality types engaged in relatively less tattooing. Clinical syndromes associated with more tattooing included Mania, Drug Abuse, Post-Traumatic Stress disorder, and Thought Disorder. 相似文献
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Pinheiro MF Cainé L Pontes L Abrantes D Lima G Pereira MJ Rezende P 《Forensic science international》2005,148(2-3):221-223
Allele frequencies of sixteen autossomal short tandem repeats (STRs), D3S1358, VWA, D16S539, D8S1179, D21S11, D18S51, TH01, FGA, D5S818, D13S317, D7S820, TPOX, CSF1PO, Penta D, Penta E (included in the PowerPlex 16 kit), and the SE33 (PowerPlex ES Monoplex System SE33) were determined in a sample of 200 healthy unrelated individuals from the north of Portugal. 相似文献