Recovery of human DNA profiles from poached deer remains: A feasibility study

Poaching is a crime that occurs worldwide and can be extremely difficult to investigate and prosecute due to the nature of the evidence available. If a species is protected by international legislation such as the Convention on International Trade in Endangered Species of Wild Fauna and Flora then simply possessing any part of that species is illegal. Previous studies have focused on the identification of endangered species in cases of potential poaching. Difficulties arise if the poached animal is not endangered. Species such as deer have hunting seasons whereby they can legally be hunted however poaching is the illegal take of deer, irrespective of season. Therefore, identification of deer alone has little probative value as samples could have originated from legal hunting activities in season. After a deer is hunted it is usual to remove the innards, head and lower limbs. The limbs are removed through manual force and represent a potential source of human touch DNA.We investigate the potential to recover and profile human autosomal DNA from poached deer remains. Samples from the legs of ten culled deer were obtained (40 in total) using minitapes. DNA from samples was extracted, quantified and amplified to determine if it would be possible to recover human STR profiles.Low quantification data led to the use of an extended PCR cycling protocol of 34 cycles. Samples from seven deer amplified, however some samples were excluded from further analysis due to ‘drop in’ alleles or the low level of successfully amplified loci. Samples from five deer could be further analysed and gave match probabilities ranging from 6.37 × 10^{− 3} to 9.53 × 10^{− 11}.This study demonstrates the potential of recovering human touch DNA from poached animal remains. There is the potential for this test to be used in relation to other species of poached remains or other types of wildlife crimes. This is the first time, to our knowledge, that human STR profiling has been successfully applied to touch DNA in regards to simulated wildlife crime.     

Mother-daughter dyads view the family: Associations between divergent perceptions and daughter well-being

Divergent perceptions (or disagreements) within the mother-daughter dyad and the association of such divergence with daughter's affective and behavioral well-being were examined in the current study. One hundred sixty-one mother-daughter dyads (daughters aged 14–18 years; mothers aged 37–59 years) completed paper- and-pencil measures assessing their perceptions of family cohesion and family conflict; daughters also rated their own depressive affect and dieting behavior. While the means for groups of mothers and daughters on family cohesion and conflict were similar, dyads varied substantially in their level of agreement. Disagreements on family cohesion were associated with daughter dieting behavior; maternal employment status was more highly associated with daughter depressive affect than either family conflict or cohesion. Findings are discussed in terms of their implications for studying the divergent perceptions of family members, and for family systems and relationship approaches to understanding the family.The research presented in this paper was supported by the W. T. Grant Foundation and the National Institutes of Health. Portions of this paper were presented at the 3rd biennial meetings of the Society for Research on Adolescence, Atlanta, Georgia.Received Ph.D. in Child Psychology from the University of Minnesota. Research interests include the interplay among developmental processes during transitions into and out of adolescence.Received Ph.D. in Sociology from the University of Minnesota. Research interests include familial adaptation to chronic illness and the role of family processes in self-image and depression during adolescence.Received Ph.D. from the University of Pennsylvania. Her research interests include girls' psychological adaptation to pubertal change, biosocial aspects of female reproductive events, and development of biologically and socially at risk children and adolescents.     

Building a Negotiation Research Agenda for the Millennium

Negotiation Journal -     

The experience of breast development and girls' stories about the purchase of a bra

Girls' experiences of secondary sexual characteristic development were explored via direct interview and protective techniques. In a pilot study (Study 1), 30 White middle to upper middle class 6th–8th-grade girls were interviewed about how they felt about breast and pubic hair growth, their comfort discussing these events, and their information acquisition. Breast growth was perceived as more important than hair growth, in part because the former is perceived as a more public event. Few girls reported intensely negative feelings to either change. Since responses to the direct questions were limited, 80 White 6th–9th graders were asked to tell a story (using a semi structured interview technique) about a picture of an adolescent girl, adult female, and adult male in which the adult female was taking a bra out of a shopping bag (Study 2). Affect, attitudes, and character alliances were coded. More girls attributed negative feelings to the father than to the mother character. Almost all girls describe the adolescent in the story as embarrassed. The daughter and father characters were described as uncomfortable more often than the mother character (50% vs. 10%). Positive maternal affect in the stories was associated with more advanced pubertal status, positive body images, more positive emotional tone, and lower anxiety scores. Affect ascribed to the father and daughter characters was less likely to be associated with girls' psychological functioning. Results are discussed in term sof the possible role of puberty upon parent-child relationships and communication.We wish to thank the W. T. Grant Foundation and the National Institute of Child Health and Human Development for their generous support of our research. This article was written while the first author was a Visiting Scholar at the Russell Sage Foundation; the support of the foundation is appreciated.Received Ph.D. from the University of Pennsylvania.Received M.D. from Columbia University.