The use of Hemastix® severely reduces DNA recovery using the BioRobot® EZ1

Abstract: The choice of reagents for presumptive tests for blood, and subsequent extraction methodologies, can significantly affect both the quantity and quality of purified DNA. Blood samples directly tested with Hemastix^® yielded <1% of the DNA recovered from untested samples when purified using the Qiagen BioRobot^® EZ1 and EZ1^® DNA Investigator kit. Full short tandem repeat profiles were obtained from both tested and untested samples, suggesting that the Hemastix^® reagent(s) affect DNA binding, rather than produce DNA damage. The Hemastix^® inhibition of DNA yield could be overcome by the addition of MTL buffer to the sample prior to extraction. Laboratories may wish to modify current procedures for extracting blood samples, utilize other extraction/purification methodologies, or inform their submitting agencies to avoid direct exposure of questioned bloodstains to Hemastix^® reagents.     

A fast preparation of skeletal materials using enzyme maceration

The current study investigates the removal of soft tissues from mice and rats by the use of three different proteases and one lipase from Novozymes A/S. The results demonstrate the enzyme maceration to be remarkably fast (1-3 h) compared to the traditional warm-water procedure, which requires up to several days. In addition, the enzyme maceration eliminates the odor problem associated with the traditional procedure. It is shown that stirring of the enzyme maceration bath is the main factor which determines the speed of the maceration. For mice, the time required for enzyme maceration can vary from 1 to 8 h depending on the stirring speed. The method investigated here allows preparation of skeletal material in an essentially odorless way within a matter of hours, making the method useful in particular for forensic science, private conservation workshops, and educational purposes.     

The economic effects of federalism and?decentralization—a cross-country assessment

This paper explores the idea that institutional details matter and that attempts to estimate the economic effects of federalism by employing a simple dummy variable neglect potentially important institutional details. Based on a principal component analysis, seven aspects of both federalism and decentralization are used as variables for explaining differences in (1) fiscal policy, (2) government effectiveness, (3) economic productivity, and (4) happiness. The results show that institutional details do, indeed, matter. Different aspects of federalism impact on the outcome variables to different degrees. This study adds to our knowledge on the transmission mechanisms of federalism and decentralization.     

The protective role of coping and social resources for depressive symptoms among young adolescents

Early adolescence is a period of the life course involving high levels of challenge that are stressful for some, perhaps resulting in depressive symptoms. In this study, adolescents were divided into four groups based on indices of depression and negative life events. Group differences in coping style, mastery, optimism, and social resources as well as group differences in patterns of change were investigated. Participants were 458 adolescents in sixth and seventh grade from a rural working class community. Subjects were assessed twice over a one-year period. Analyses revealed that the four groups were characterized by different levels of coping and social resources. Asymptomatic youth reported higher levels of optimism, mastery, active coping, and more positive relationships with parents and peers than did symptomatic adolescents. These same characteristics distinguished the resilient adolescents from the vulnerable adolescents, suggesting potential stress-buffering effects. One year later, the adolescents who were low on both depressive symptoms and negative life events continued to report more individual and contextual resources than the adolescents in the other groups.This research was supported by a grant from the William T. Grant Foundation (8912789) to Anne C. Petersen, Principal Investigator. The writing of this article was supported by The National Institute of Mental Health Research Training Grant 5 T32 MH18387-06 in Child Mental Health/Primary Prevention.Received Ph.D. from the Department of Human Development and Family Studies at Pennsylvania State University, and did a postdoctoral fellowship in prevention research at Arizona State University. Research interests include adolescent development, prevention, and community psychology.(on leave to the National Science Foundation). Received Ph.D. from the University of Chicago. Research interests include biopsychosocial development at adolescence, adolescent depression and interventions to prevent it, gender issues, and developmental methodology.     

A self-image questionnaire for young adolescents (SIQYA): Reliability and validity studies

This article describes a questionnaire measure of self-image designed for young adolescents. It represents a downward extension of the Offer Self-Image Questionnaire and utilizes nine scales from that instrument: Emotional Tone, Impulse Control, Body Image, Peer Relationships, Family Relationships, Mastery and Coping, Vocational/Educational Goals, Psychopathology, and Superior Adjustment. This 98-item questionnaire elicits responses on a 6-point Likert-type scale. The alpha coefficients for each scale are high, indicating a high degree of internal consistency among the items. The validity of this instrument is examined through factor analyses and through the association of these scales with other measures of self-image. The results suggest that this questionnaire provides a useful way to assess self-image among young adolescents.This research was supported by Grant MH 30252/38142 to Anne Petersen.Received Ph.D. from the University of Chicago in 1973. Research interest is biopsychosocial development in adolescence, with a focus on sex differences.Research interests are vocational development and contextual influences on development in early adolescence.Research interests are the influences of adolescent and parent development on family relationships.Received M.D. from the University of Chicago. Research interests are the psychology and psychopathology of adolescents.Current research interests are social cognition and peer relationships during early adolescence.     

Community‐Based Participatory Research as a Tool for Policy Change: A Case Study of the Southern California Environmental Justice Collaborative

In 2000, a regional rule governing maximum individual cancer risk from stationary facilities in Southern California was dramatically altered, reducing allowable risk levels by 75%. This article uses a case study approach to explore the role of a community‐based participatory research (CBPR) partnership, the Southern California Environmental Justice Collaborative, in producing research and helping spearhead policy advocacy leading to this policy change. It also highlights the role of the collaborative in helping to change the framing of the issue from individual to cumulative risk assessment, so that the regulatory agencies began to reflect this broader thinking in their policymaking. The collaborative's structure and methodology, regional focus, relationships with key decision makers, and its reputation as an important source of both credible science and “people power” were seen as contributing to its effectiveness. The role of contextual factors including a recovering and more regulation‐friendly economy also is highlighted, as are key barriers faced. Implications for other community–academic partnerships working to address regional and statewide public policy are discussed.     

Statistical analysis of barefoot impressions

Comparison of the shapes of barefoot impressions from an individual with footprints or shoes linked to a crime may be useful as a means of including or excluding that individual as possibly being at the scene of a crime. The question of the distinguishability of a person's barefoot print arises frequently. This study indicates that measurements taken from the outlines of inked footprint impressions show a great degree of variability between donors and a great degree of similarity for multiple impressions taken from the same donor. The normality of the set of measurements on footprint outlines that we have selected for this study is confirmed. A statistical justification for the use of the product rule on individual statistical precisions is developed.     

Cognitive mediators of ego functioning in adolescence

It has often been assumed that a relationship exists between higher levels of cognitive functioning, particularly formal operations, and mature ego functioning in adolescence. This research examined the relationships between ego functioning and two domains of operational thinking: social interpersonal reasoning and physical-mathematical reasoning in 139 high school seniors. Subjects were given two measures of physical-mathematical reasoning, two measures of interpersonal reasoning, and the Sentence Completion Test of ego functioning, as well as a measure of verbal intelligence. Results indicated significant differences between males and females in patterns of correlations as well as in patterns of relationships in a causal analysis. Ego functioning was predicted by interpersonal reasoning for females and by physical-mathematical reasoning and verbal intelligence for males.This research represents a portion of the doctoral dissertation completed by the senior author in 1981. The research was supported by a grant to A. Petersen from the Spencer Foundation.Received Ph.D. from University of Illinois at Chicago. Current interests are sex-related differences in adolescent ego development and psychosocial variables in adolescent chronic illness.Received Ph.D. from the University of Chicago. Current interest is biopsychosocial development in adolescence, primarily early adolescence.Research Affiliate, Laboratory for Study of Adolescence, Michael Reese Hospital. Received Ph.D. from University of Chicago. Current interest is sex-related differences in the psychological effects of puberty.Research Associate, Laboratory for the Study of Adolescence, Michael Reese Hospital; Program Associate, Health Program, MacArthur Foundation. Received Ph.D. from Syracuse University. Current interest is sex-related differences in socialization.