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Laurence E. Lynn 《Journal of policy analysis and management》1982,1(4):482-495
Public management requires a diffuse set of activities that extend far beyond the simple activities once encompassed in the study of public administration. The study of public management is hampered by the lack of theory to guide research on the roles and contributions of public managers. By viewing the government executive as a player in a game—more accurately, as a player in many games occurring simultaneously and at different levels, each with its own rules of play—we can begin to gain an integral view of the reality in which they operate. 相似文献
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Forensic mitochondrial DNA analysis of 691 casework hairs 总被引:1,自引:0,他引:1
A five year retrospective review of mitochondrial DNA (mtDNA) analysis on 691 casework hairs was carried out. A full or partial mtDNA profile was obtained for > 92% of hairs. With increasing age of the hair, the likelihood of obtaining a full profile decreased, although "mini-primer sets" could often be used to capture a partial profile. With increasing color and diameter of the hair, the likelihood of obtaining a profile increased. Full or partial profiles were obtained on more than 80% of 114 hairs < or = 1.0 cm. Mixtures were observed in 8.7% of hairs tested; mixtures increased with the age of the hair and were presumed to be due to exterior surface contamination that could not be sufficiently cleaned prior to extraction, since the overall level of laboratory contamination was low. The frequency of sequence heteroplasmy was 11.4%, and both hot-spot and novel sites were observed. In about one-third of these observations, another sample in the case showed either the same heteroplasmic site or a nucleotide substitution at that site. 相似文献
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