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41.
Quantification of Morphine,Codeine, and Thebaine in Home‐Brewed Poppy Seed Tea by LC‐MS/MS
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Deborah Powers M.S. Stephen Erickson M.D. Madeleine J. Swortwood Ph.D. 《Journal of forensic sciences》2018,63(4):1229-1235
Recently, medical examiners reported two cases of a 21‐year‐old male and 24‐year‐old male with high amounts of morphine in their blood at autopsy. It was suspected that the decedents ingested lethal amounts of morphine from home‐brewed poppy seed tea. No studies to date have investigated opium alkaloid content extracted from poppy seeds by home‐brewing methods. Various poppy seed products were purchased from online sources and extracted with four home‐brewing methods representative of recipes found on drug user forums. Morphine, codeine, and thebaine were quantified in the tea extracts by liquid chromatography‐tandem mass spectrometry using a validated analytical method. Morphine, codeine, and thebaine concentrations from seeds were <1–2788 mg/kg, <1–247.6 mg/kg, and <1–124 mg/kg, respectively. Alkaloid yield varied between extractions, but regardless of extraction conditions, lethal amounts of morphine can be rinsed from poppy seed coats by home‐brewing methods. 相似文献
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What determines the allocation of voting weights to member states in international organizations? What drives the seat and voting weight allocation in the European Parliament (EP) and in the Council of the European Union (EU)? Our objective in this article is to develop a universal logical model and to demonstrate that the resulting equation indeed captures negotiated outcomes on seat and voting weight allocations in EU institutions from their beginning. We predict seat and voting weight allocations for both the EP and the Council of the EU within one general model. Hence, we do not employ actual data on seat allocations or voting weights in either the EP or the Council of the EU, but instead, use logical constraints exclusively, as posed by the following elements: the total number of seats/voting weights ( S ), the number of member states ( N ) and, finally, their respective population size ( Pi ). Only our final model selection among several theoretical options is guided by empirical information. With no post hoc parameters used, our model fits both the Council of the EU and the EP rather well, over a time span of nearly 40 years. Inspired by the 'seat–vote equation' ( Taagepera, 1973 ) for seat allocation in national legislatures, the new 'seat–population equation' calculates the number ( S i ) of EP seats or Council voting weights of member state i as follows: , where n =(1/log N − 1/log S )/(1/log N − 1/log P ), P being the total population (as summed over all member states). We posit that this equation is applicable to predict outcomes in practice whenever voting weight or seat allocations in international organizations are allocated on the basis of the population shares of their component entities. 相似文献
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Munchelou M. Gomonit BS Britni Skillman PhD Madeleine J. Swortwood PhD 《Journal of forensic sciences》2024,69(2):678-687
There has been burgeoning interest in psilocybin-use for the treatment of various neurological and neurodegenerative diseases. Psilocybin is mistakenly perceived as the principal pharmacologically active compound due to its high concentrations found in magic mushrooms; however, it is the prodrug of psilocin. Despite the expanding body of clinical research seeking to understand the pharmacodynamic/pharmacokinetic properties of psilocin, and its role in inducing dramatic changes to cognitive function, there has not been a corresponding increase in the development of sensitive analytical methods that can quantify psilocin in different biological fluids. Existing analytical methods have been developed using plasma, serum, and urine as the matrix of choice, but with the unknown blood-to-plasma ratio of psilocin, any pharmacokinetic conclusions drawn solely on plasma data may be misleading. Thus, the main objective of this study is to develop the first analytical method that utilizes SPE and LC–MS/MS to quantify psilocin in human whole blood. The SPE procedure yielded a high recovery efficiency (≥89%) with minimal matrix effects. The method was validated according to ANSI/ASB 036 guidelines. Linearity was between 0.7–200 ng/mL and encompassed previously reported ranges found in plasma/serum. Bias, within- and between-run precision for all quality controls met ANSI/ASB 036 acceptability criteria. Endogenous/exogenous interferences and carryover were negligible. Psilocin stability was assessed at 4°C over 48 h and was considered stable. Although a proof-of-concept study will need to be performed to characterize the method, this analytical workflow was able to detect and quantify psilocin in human whole blood at low limits of quantification. 相似文献
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Editor in Chief,Criminal Law Forum: An International Journal; Distinguished Professor, Rutgers University School of Law, Camden, New Jersey, United States; B.A., Victoria University of Wellington 1964; LL.B., Victoria University of Wellington 1964; LL.M., Victoria University of Wellington 1967; LL.M., Columbia University 1968; J.S.D., Columbia University 1972. 相似文献
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