A new pentaplex system to study short tandem repeat markers of forensic interest on X chromosome

A new method has been optimised to amplify five X chromosome short tandem repeat (STR) markers of interest in forensic medicine: human phosphoribosyl transferase (HPRTB), DXS101, androgen receptor (ARA), DXS7423 and DXS8377. Markers were conveniently amplified in a single PCR reaction with fluorochrome-labelled primers, which allowed the analysis of fragment sizes after injection into a capillary electrophoresis system. The most common alleles of each locus were sequenced and used in a control ladder to type unknown samples.     

Results of a collaborative study of the EDNAP group regarding the reproducibility and robustness of the Y-chromosome STRs DYS19, DYS389 I and II, DYS390 and DYS393 in a PCR pentaplex format

A collaborative exercise was carried out by the European DNA Profiling Group (EDNAP) in the frame work of the STADNAP program, i.e. standardization of DNA profiling in Europe, in order to evaluate the performance of a Y-chromosome STR pentaplex, which includes the loci DYS19, DYS389 I and II, DYS390 and DYS393 and to determine whether uniformity of results could be achieved among different European laboratories.Laboratories were asked to analyze the five Y-STRs using singleplex and multiplex conditions in three bloodstains and one mixed stain (95% female and 5% male).All the laboratories reported the same results even for the mixed stain included in the exercise. This demonstrates the reproducibility and robustness of Y-chromosome STR typing even with multiplex formats and proves the usefulness of Y-STR systems for analyzing mixed stains with a male component.A total of 930 male samples from 10 different populations from Europe were also analysed for all the loci included in the pentaplex. Eight of these ten populations also included haplotype data.As for single gene analysis, haplotype diversity was higher in Germany and Italy and lower in Western European countries and Finland.Pairwise haplotype analysis shows the Finnish departure from the rest of the populations and a relatively homogeneity in the other European populations with F(ST) estimates lower than 0.05.UPGMA analysis shows an association of Western European population (Ireland, UK, Portugal and Galicia) on the one hand and central European populations on the other.     

STR data for the AmpFlSTR profiler plus loci from Macau (China).

Allele frequencies for the nine STRs included in the AmpFlSTR profiler plus kit (D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820) were estimated from a sample of 87 unrelated individuals living in the region of Macau, a territory located in the southeastern coast of China.     

STR data (CD4, CSF1PO, F13A01, FES/FPS, MBPB, TH01, TPOX) from North Portugal.

Allele frequencies for seven STRs (CD4, CSF1PO, F13A01, FES/FPS, MBPB, TH01, TPOX) were estimated from samples (sized between 300 and 940) of unrelated individuals born in North Portugal.     

How Do Base Rules Constitute a Virtual World?

International Journal for the Semiotics of Law - Revue internationale de Sémiotique juridique - The classic theory of rules and norms rooted in John Searle’s theory of institutional...     

Three kinds of preindustrial household formation system in historical Eastern Europe: A challenge to spatial patterns of the European family

In their modelling and classificatory ventures western scholars have usually mistakenly included family forms in historical Eastern Europe by induction in well-established generalizations about Russian or Balkan populations. At the same time, well into the late 1990s, most of Eastern European historians have shown no interest in studying domestic groups in socio-historical perspective. This article attempts to restate that picture through a thorough analysis of an unprecedented collection of historical household data for the late eighteenth-century Polish-Lithuanian Commonwealth, one of the largests political entities in Europe of that time. Through an application of a variety of methodologies three regional family patterns have been distinguished on the historical Polish territories, their main characteristics described and then juxtaposed against the major features of paradigmatic examples of the ‘Eastern European family type’. The results indicate that the existing models of household systems in preindustrial Europe are far too rigid to meet the diversity of residential patterns of the Eastern European serfs. Analysis of the data set on spatially, culturally and socioeconomically diverse regions has also facilitated a preliminary identification of the factors shaping these family systems. The data presented here suggests that the conventional wisdom regarding the institutional mechanisms of the Eastern European manorialism of the second serfdom as sufficient to create a homogenous pattern of family residence must be seriously questioned. In particular, what must be meticulously revised is a sweeping generalization still in practice that posits a functional link between coercive forms of labor control and complex household structures among peasant subjects.     

Marriage patterns among Lutherans from the parish of Trzebosz in the second half of the 19th century and the beginning of the 20th century

The aim of this study is to characterize marriage patterns in a rural parish of Trzebosz in the borderland between Greater Poland (Wielkopolska) and Silesia in the years 1855–1913. A total of 343 data on marriages were gathered from the parish registers. The percentage of Lutheran–Catholic marriages was calculated. The distribution of age at marriage by martial status was assessed. The intensity of endogamy and exogamy was calculated as well as the coefficients of exogamy and biological polygamy. The annual rhythm of marriages was determined. In Trzebosz mixed marriages accounted for 3.94% in 1855–1899 and 14% in 1900–1913. The average age of brides and grooms was 25.36 and 26.22 years, while of widows and widowers they were 37.26 and 42.35 years, respectively. The average age of brides and grooms declined over time. In 1855–1899 the levels of endogamy and exogamy outside the parish were 19% and 81%, respectively. In 1900–1913 exogamy outside the parish decreased to 62%, while endogamy increased to 37%. The average mating distances for all marriages and exogamous ones were 24 and 42 km, respectively, in 1855–1899, while for 1900–1913 the respective figures were 7.4 and 8.7 km. The coefficient of exogamy declined from 0.78 in 1855–1899 to 0.67 in 1900–1913, while the coefficient of biological polygamy grew from 1.19 to 1.30 between those periods. The religious factor, apart from an economic strategy, shaped the annual rhythm of marriage. The outflow of the parishioners from Trzebosz in the early 20th century led to its ageing.     

The failure of the participatory democracy in the Czech republic

Following the breakdown of the Soviet system, the new East European elites faced the problem of defining and building democratic institutions. This problem was not a purely institutional one, however. During the transformation process, different conceptions of democracy appear and often become critical issues for political competition. Based on the Czech case, this article aims to understand how and why one particular conception of democracy becomes dominant during a process of regime change. Personified by the two ‘Vaclavs’ in the Czech political arena (Havel and Klaus), divergent perspectives on democracy exist in the Czech Republic, having concrete consequences for the practice of politics. These conceptions (referred to here as ‘participatory’ and ‘majoritarian') dramatically differ in their perception of the role of the citizen in a liberal democracy. This article identifies and describes these two different conceptions of democracy in the present and past Czech Republic. It explores the sociological conditions of their emergence in order to understand the failure of the participatory model of democracy with respect to the alternative, majoritarian, vision of democracy.     

Capital and Labour Mobility and their Impacts on Mexico's Regional Labour Markets

Abstract

This paper studies the effects of capital and labor mobility on real wages across Mexican states for the period 1997–2006. Employing dynamic panel data methods, we find: (1) strong positive effects on real wages from foreign direct investment (FDI) and from migration; (2) domestic and foreign migration provide similar wage effects; and (3) alternative partitions indicate that real wages are more sensitive to FDI-related fluctuations across states with relatively lower wages and migration levels. Overall, these results provide support that real wages respond positively to fluctuations in capital flows and labour movements as predicted from the theory.