Characterization of human DNA in environmental samples

Environmental samples from indoor surfaces can be confounded by dust, which is composed largely of human skin cells and has been documented to contain roughly tens of micrograms of total DNA per gram of dust. This study complements previous published work by providing estimates of the quantity of amplifiable human DNA found in environmental samples from a typical indoor environment, categorized by the intensity of human traffic and visible quantity of dust. Dust was collected by surface swabbing standard 576 cm² areas in eight locations, and evaluated for total DNA quantity, presence of human DNA (mitochondrial and nuclear loci using conventional PCR), quantity of human nuclear DNA using quantitative PCR, and STR analysis. The total DNA content of 36 dust samples ranged from 9 to 28 ng/cm², and contained 0.2–1.1 pg/cm² of human DNA. Overall, human DNA was detected in 97% of 36 dust samples and 61% of samples yielded allele distributions of varying degrees of complexity when subjected to STR analysis. The implications of this study are twofold. First, the presence of dust in evidence can be a significant contamination source in forensic investigations because the human DNA component is of sufficient quality and quantity to produce allele calls in STR analysis. This can be effectively managed by implementing stringent protocols for collection and analysis of potential biological samples. A second implication is the use of dust as a source of evidence for identification of inhabitants within a defined location. In the latter case, a number of additional studies would be necessary to identify relevant pretreatments for environmental dust samples and to develop the necessary deconvolution techniques to separate the composite genotypes obtained.     

What Gets Measured,Gets Done: Understanding and Addressing Middle‐Class Challenges

Middle‐class families face a range of challenges, including uneven income growth, imposing child care costs, and affordability gaps for higher education. The ideal policies by which policy makers and public administrators can aid the middle class are far from obvious. Policy solutions are likely to mirror our government and population, meaning that they will be decentralized and varied. Achieving a “growing and thriving middle class” requires understanding the composition of the middle class across the country. Benchmarking and measuring the middle‐class condition at the state and substate levels is critical to crafting and adopting effective policy solutions. This Viewpoint essay highlights the Colorado context to demonstrate the measurement of the middle class and tracking of its lived experiences.     

Physical purity feminism and state medicine in late nineteenth-century england

Abstract

Late nineteenth-century England saw the development of a number of campaigns and social movements which were connected by both a hostility towards the medical profession and by the use of discourses of purity and sanitary reform. This article explores the involvement of women within these movements, analysing their activism as an aspect of social purity feminism. It argues that many of these movements drew on widespread female anxiety regarding male violence – both physical and sexual – towards women. The anti-medical feminists claimed that some pieces of ‘sanitary’ legislation represented a state-sanctioned violation of the bodies of women and children. Finally, this article analyses the use made, by some of these activists, of the discourses of sanitary reform to challenge the gender ordering associated with the reason/nature dualism in Victorian society.     

Power,agency and structure: An examination of Steven Lukes

The dynamic between violence and politics in the American South during the Civil Rights Movement is analyzed, focusing on the relationship between racist terror and the state. The study explores how blacks in the rural South tried to defend themselves before civil rights, when white supremacists dominated local government and federal authorities ignored lynch murders. The article traces the development of Martin Luther King, Jr.'s nonviolent resistance strategy, and the long struggle to force the state to combat racist violence, one of the Civil Rights Movement's most significant achievements.     

Gender, Civil War and National Identity: Women Partisans during the Greek Civil War 1946–1949

This paper focuses on the recovery of the experience of partisan women who fought in the lines of the communist-led Greek Democratic Army (GDA) during the Greek Civil War (1946–1949). It is an extract from a research project which examines the extent to which the symbolism of Greek women in arms has informed the various waves of feminist discourse which have emerged since the founding of the modern Greek nation state. More specifically this work employs the image of the Greek woman warrior as an analytical category to investigate the relationship between militarism, nationalism and Greek feminist politics in relation to key nation-building conflicts of the modern Greek period. As such it belongs to the realm of scholarship informed by an understanding of 'gender' and 'nation' as constructed and contested relational systems of cultural and social meanings. Together the two systems not only shape the political culture in historically specific ways but also legitimate and limit the access of (groups of) people — women as well as men — to national movements as well as to the resources of the nation-state. This paper concentrates specifically on the heavily mythologised women of the GDA. It pays special attention to the historical association of their rebellion with national citizenship rights in post-war Greece.