Scientific standards for studies in forensic genetics

Forensic molecular genetics has evolved from a rapidly developing field with changing technologies into a highly recognized and generally accepted forensic science, leading to the establishment of national DNA databases with DNA profiles from suspects and convicted offenders. DNA evidence has taken a central role by carrying a significant weight for convictions, as well as by excluding innocent suspects early on in a criminal investigation. Due to this impact on the criminal justice system, guidelines for research in forensic genetics have been introduced already since many years. The most important issues regarding the selection and definition of typing systems both for paternity testing and for forensic identification, the criteria for technical and biostatistical validation, as well as the use of mitochondrial DNA analysis are summarized and discussed.     

The politics of flexing soybeans: China,Brazil and global agroindustrial restructuring

The political geography of the global soybean complex is shifting. While the complex has long been controlled by US-based transnational corporations, new agribusiness actors, business logics and power relations rooted in South America and East Asia are emerging, based in part on commodity flexing. We explore how soybean flexing is shaping and being shaped by global restructuring of the soybean processing industry. Using the divergent histories and uses of soy in China and Brazil, we propose that in order to understand the changing soy landscape, we must examine the relationships between soy's multiple-ness and flexible-ness, the political economy of soy processing, and the relationships between crop ‘flexors’ – those powerful firms that control the soy complex – with each other and with governments. We demonstrate that the agribusiness actors who are gaining more control over the soy complex are doing so in part through flexing, and that the ability to flex may ultimately determine the trajectory of global agroindustrial restructuring. Finally, we raise questions and make suggestions for further research on flex crops.     

Injuries and fatalities caused by gas-/warning weapons

Detailed report of 5 cases of violation with gas or warning firearms, 3 of them lethal. In one case a modified weapon (elongated barrel) and steel bullets were used, resulting in shot of the head with retained missile and lethal bleeding. In the other cases, regular weapons (8 or 9 mm cartridges) were used for contact shots; violation occurred only by the effect of powder gases. One of these cases was a suizide (lethal temporal gunshot), in the other cases shots by another person: a) shot against the arm with large soft-tissue damage (survived); b) and c) shot against the neck with exceeding rupture of the soft tissues, minimal damage of the neck arteries and in one cases bilateral tears of the hypopharynx. In this case a delayed death after primarily well operated injury occurred after 12 days by bleeding into the airways from ruptured external carotid artery.     

Self-injury or attempted murder--analysis of an unusual case

An unusual case of self-injury involving several, partly deep penetrating stab wounds is described. A young emotionally disturbed consulting-room assistant accused her husband of having inflicted the injuries upon her in the course of an argument over custody of the children. An analysis of the injury pattern showed clear indications of self-injury and willfully transferred blood from punctures in the woman's fingertips to the clothes of the accused. The case demonstrates that in cases involving members of a medical profession and individuals with psychiatric disorders, the possibility of highly unusual types of self-injury should not be excluded. It further emphasizes the importance of a physical examination performed by a medico-legal specialist.     

Results of collaborative study regarding the standardization of the Y-linked STR system DYS385 by the European DNA Profiling (EDNAP) group.

Y-chromosome linked short tandem repeat (STR) loci are inherited as a closely linked haplotype, which appears to remain stable in a given paternal lineage over many generations. In forensic cases, Y-linked STRs are particularly useful for the identification of human remains as well as in rape cases with mixed male/female stain samples. DYS385 is derived from tandemly duplicated segments of the Y chromosome thus giving rise to two fragments of variable length which do not behave like alleles but genotypes. The European DNA Profiling (EDNAP) group has carried out a collaborative exercise among 14 participating laboratories using DYS385 for typing of five unknown bloodstains and a control sample. Furthermore, population data from eight different European countries with samples sizes between 91 and 150 male individuals were collected. The results confirm previous observations that DYS385 is one of the most informative Y-linked STR loci. It could also be demonstrated that reproducible results can be obtained independently from the electrophoretic separation and detection methods used. Thus DYS385 may serve as a useful complementation to the routinely used autosomal STR systems in special cases.     

Analysis of psilocybin and psilocin in Psilocybe subcubensis Guzmán by ion mobility spectrometry and gas chromatography-mass spectrometry

A new method has been developed for the rapid analysis of psilocybin and/or psilocin in fungus material using ion mobility spectrometry. Quantitative analysis was performed by gas chromatography-mass spectrometry after a simple one-step extraction involving homogenization of the dried fruit bodies of fungi in chloroform and derivatization with MSTFA. The proposed methods resulted in rapid procedures useful in analyzing psychotropic fungi for psilocybin and psilocin.