Analysis of biofluids for flunitrazepam and metabolites by electrospray liquid chromatography/mass spectrometry

A rapid and sensitive liquid chromatography/electrospray ionization mass spectrometry (LC/MS) procedure has been developed for the analysis of biofluids containing flunitrazepam and its metabolites. Specimens were spiked with deuterated analogs of the analytes. Urine specimens were enzymatically hydrolyzed and blood specimens were untreated. Extractions were carried out using CleanScreen DAU SPE cartridges. The drugs were separated on a C18 column using a methanol-water-ammonium hydroxide (60:40: 0.03 v/v) mobile phase. After determination of base peaks using full scan mass spectrometry, the mass spectrometry method was optimized to operate in selected-ion monitoring (SIM) mode for the base peak of each analyte. Positive findings were confirmed by LC/MS/MS using the same mobile phase and column. This analytical procedure allows for the detection of low levels of flunitrazepam and metabolites in biofluids. It is useful for ascertaining the role of flunitrazepam in cases of drug-facilitated sexual assault.     

Older workers' progression from private disability benefits to Social Security disability benefits

People with medical conditions that limit their ability to work tend to receive short-term disability benefits initially and may then move to long-term and eventually to permanent disability benefits. The progression of older workers (those aged 55 to 64) along that continuum of benefits is documented here with data from a large disability insurance company. The data show that older workers who receive short-term medical disability benefits are three times as likely as younger workers to progress to receipt of Social Security Disability Insurance (SSDI) benefits, although a slight reversal of that trend occurs as workers pass age 62. Musculoskeletal conditions are the most frequent basis of short-term disability claims among older workers, with circulatory conditions running a close second. Furthermore, although all medical conditions are more likely to lead to SSDI benefits among older workers, circulatory conditions do so most frequently. This article discusses industry standards for the management of disability claims at each level of severity. It also addresses common and emerging disability management practices that may reduce the likelihood of impaired workers developing long-term or permanent financial dependence on disability benefits programs.     

Dagher redux: searching for the missing pieces

The most relevant question for any joint venture is, when does the relationship become a jointventure and not simply a price-fixing cartel? With respect to this question, this Article juxtaposes Texaco, Inc. v. Dagher, 126 S. Ct. 1276 (2006), against years of contrary precedent. In Dagher, the Court altered the seemingly settled foundation of antitrust law by changing its view on past holdings and abandoning the ancillary effects doctrine. The Article provides an outline of key holdings prior to Dagher, as well as a discussion of the issues that can arise as joint ventures are formed. Additionally, the authors examine how the decision altered the foundation of joint venture law in the United States. In particular, the Article exposes several important antitrust concerns relating to joint ventures that the Supreme Court did not address in Dagher. Perhaps the most perplexing issue of Dagher is whether the venture at issue would have survived analysis under the Federal Trade Commission's "continuum" approach.     

On the prehistory of the German Society of Legal Medicine

The paper gives a review on the foundation of local associations of legal medicine and their journals at the beginning and in the mid of the 19th century in Germany before the German Society of Legal Medicine was founded.     

Gesellschaftskritik und soziologische Aufklärung

Berliner Journal für Soziologie - Differenz versus Vermittlung, beobachtete, polykontexturale versus monokontexturale, falsche Realität — mit dieser Formel lassen sich die...     

Pharmacogenomics as an aspect of molecular autopsy for forensic pathology/toxicology: does genotyping CYP 2D6 serve as an adjunct for certifying methadone toxicity?

Pharmacogenomics, applied as an aspect of molecular autopsy, may be used as an adjunct for certifying methadone fatalities. Methadone is metabolized by cytochrome P-450 (CYP) 1A2, 3A4, and 2D6. We hypothesized that methadone toxicity may be partially due to CYP 2D6 *3, *4, and *5 variant alleles, resulting in poor drug metabolism. A retrospective analysis was performed on covariables and risk factors of 21 methadone cases from the Milwaukee County Medical Examiner's Office (1998-2000). PCR genotyping showed: one heterozygous for 2D6*3, two homozygous for 2D6*4, five heterozygous for 2D6*4, and one heterozygous for both 2D6*3 and *4. This limited number of cases showed that the prevalence of poor metabolizer was higher but not significantly different from that of a control group (n = 23) (P > 0.05, Fisher Exact Test). Thus, CYP 2D6 mutations may not yet be directly associated with methadone toxicity. However, pharmacogenomics, complementing other case findings, served as an adjunct in interpreting methadone toxicity of poor and intermediate metabolizers.     

Background correction in forensic photography. I. Photography of blood under conditions of non-uniform illumination or variable substrate color--theoretical aspects and proof of concept

The combination of photographs taken at two or three wavelengths at and bracketing an absorbance peak indicative of a particular compound can lead to an image with enhanced visualization of the compound. This procedure works best for compounds with absorbance bands that are narrow compared with "average" chromophores. If necessary, the photographs can be taken with different exposure times to ensure that sufficient light from the substrate is detected at all three wavelengths. The combination of images is readily performed if the images are obtained with a digital camera and are then processed using an image processing program. Best results are obtained if linear images at the peak maximum, at a slightly shorter wavelength, and at a slightly longer wavelength are used. However, acceptable results can also be obtained under many conditions if non-linear photographs are used or if only two wavelengths (one of which is at the peak maximum) are combined. These latter conditions are more achievable by many "mid-range" digital cameras. Wavelength selection can either be by controlling the illumination (e.g., by using an alternate light source) or by use of narrow bandpass filters. The technique is illustrated using blood as the target analyte, using bands of light centered at 395, 415, and 435 nm. The extension of the method to detection of blood by fluorescence quenching is also described.     

What About Syria?

The following is the edited text of the presentations at the September 30, 1999, meeting of the Sadat Forum at Brookings, cohosted by Richard Haass, vice-president and director of Foreign Policy Studies at the Brookings Institution, and Shibley Telhami, the Anwar Sadat Chairfor Peace and Development at the University of Maryland.     

Women in the public service - a thirty-year perspective

Abstract: Over the last thirty years, there has been a substantial improvement in the representation of women throughout the federal public service. The expectations of and about women in the workplace have also changed immeasurably. However, there are still important differences in the way men and women deal with their environment. The different ways in which men and women are socialized continue to have an impact on women's experience in the workplace. There are still too many places in the public service where a traditional “competitive” (some would say “male”) approach to management prevails. The contrasting “integrative” style of management, which values team-building, participation, responsiveness, and the ability to integrate various points of view, more closely reflects the way in which many women have been socialized and behave in the work environment. It is also the style valued in “well-performing organizations.” In addition, there are important differences in the way many men and women communicate. These differences can lead to misinterpretation and misunderstanding, and, in a culture where “toughness” is valued, this can sometimes result in men underestimating the competence and effectiveness of women. What is required is for management to move beyond employment equity, where the emphasis is on increasing the representation of women and minorities, to a focus on valuing and managing the diversity that women and minority groups bring to the workplace, so that full advantage can be taken of the variety of perspectives, skills and abilities of all employees. Sommaire: Au cours des trente dernières années, la représentation des femmes dans la fonction publique fédérale a connu une amélioration considérable. Ce qu'on attend des femmes sur les lieux de trevail anisi que leurs propres attentes ont grandement évolué. La manière dont les hommes et les femmes réagissent à leur milieu resrte cependant assez différente. La socialisation différente des hommes et des femmes continue à se répercuter sur l'expérience des femmes au travail. Encore aujourd'hui, de nombreux secteurs de la fonction publique font appel à une gestion «compétitive» classique (certains diraient «masculine»). Par contre, le style de ges tion «intégrateur» qui met l'accent sur l'esprit déquipe, la participation, l'ouverture d'esprit et la capacité d'intégrer divers points de vue, reflète de plus prè la manière dont de nombreuses femmes ont été socialisées et dont elles se comportent au travail. D'ailleurs, c'est aussi le style que préfèrent les «organisations performantes». De plus, la manière dont communiquent les hbommes et les femmes peut différer considérablement, ce qui risque de mener à des malentendus et à des mauvaises interprétations; dans une culture ouG l'on admire la «poigne», les hommes en viennent parfois à sous-estimer la compétence et l'efficacité des femmes. La hommes en viennent parfois à sous-estimer la compétence et l'efficacité des femmes. La gestion doit main tenant aller au-delà de l'équité en matière d'emploi, qui met l'accent sur l'augmentation de la représentation des femmes et des minorités, pour gérer la diversité que les femmes et les groupes minoritaires apportent au miliey du travail afin de profiter pleinement de la variété de perspectives, de talents et d'aptitudes de tous les employés.