Internal validation of the AmpFlSTR Yfiler amplification kit for use in forensic casework.

Y-chromosomal short-tandem repeat (Y-STR) amplification has been used in forensic casework at the Bureau of Criminal Apprehension (BCA) Forensic Science Laboratory since 2003. At that time, two separate amplifications were required to type the SWGDAM recommended loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS438, and DYS439). The Yfiler kit coamplifies these loci as well as DYS437, DYS448, DYS456, DYS458, DYS635, and Y GATA H4. The Yfiler kit was validated following the internal validations outlined in the SWGDAM revised validation guidelines. Our studies show that 0.125 ng of male DNA will generate a complete 17 locus profile and that as little as 0.06 ng of male DNA yields an average of nine loci. In the male-male mixtures, a complete profile from the minor component was detected up to 1:5 ratio; most of the alleles of the minor component were detected at a 1:10 ratio and more than half the alleles of the minor component were detected at a 1:20 ratio. Complete YSTR profiles were obtained when 500 pg male DNA was mixed with female DNA at ratios up to 1:1000. At ratios of 1:5000 and 1:10,000 (male DNA to female DNA) inhibition of the YSTR amplification was evident. The YSTR results obtained for the adjudicated case samples gave significantly more information than the autosomal results. Our studies demonstrate that the Yfiler kit is extremely sensitive, does not exhibit cross-reactivity with female DNA, successfully types male DNA in the presence of overwhelming amounts of female DNA and is successful in typing actual forensic samples from adjudicated cases.     

Internal Validation of the AmpFlSTR Yfiler™ Amplification Kit for Use in Forensic Casework

Abstract:  Y-chromosomal short-tandem repeat (Y-STR) amplification has been used in forensic casework at the Bureau of Criminal Apprehension (BCA) Forensic Science Laboratory since 2003. At that time, two separate amplifications were required to type the SWGDAM recommended loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS438, and DYS439). The Yfiler™ kit coamplifies these loci as well as DYS437, DYS448, DYS456, DYS458, DYS635, and Y GATA H4. The Yfiler™ kit was validated following the internal validations outlined in the SWGDAM revised validation guidelines. Our studies show that 0.125 ng of male DNA will generate a complete 17 locus profile and that as little as 0.06 ng of male DNA yields an average of nine loci. In the male–male mixtures, a complete profile from the minor component was detected up to 1:5 ratio; most of the alleles of the minor component were detected at a 1:10 ratio and more than half the alleles of the minor component were detected at a 1:20 ratio. Complete YSTR profiles were obtained when 500 pg male DNA was mixed with female DNA at ratios up to 1:1000. At ratios of 1:5000 and 1:10,000 (male DNA to female DNA) inhibition of the YSTR amplification was evident. The YSTR results obtained for the adjudicated case samples gave significantly more probative information than the autosomal results. Our studies demonstrate that the Yfiler™ kit is extremely sensitive, does not exhibit cross-reactivity with female DNA, successfully types male DNA in the presence of overwhelming amounts of female DNA and is successful in typing actual forensic samples from adjudicated cases.     

Unusual association of three rare alleles and a mismatch in a case of paternity testing

This study reports a paternity case analyzed by the AmpFlSTR Identifiler Kit (AB) in which father and daughter shared three rare alleles for D19S433, D18S51 and TH01 microsatellites. The case also showed an apparent exclusion, due to a mutation at the D3S 1358 microsatellite. Sequencing analysis was performed to assess the size of the rare alleles and to establish their structure, which revealed some molecular variations in regions flanking the motif repeats.     

Violence against women associated with arrests for sex trade but not drug charges

The current study was designed to examine associations between gender-based violence and arrests due to sex trade or drug-related charges among a statewide sample of incarcerated women in Rhode Island. Incarcerated women were asked to participate in brief pre- and posttest surveys of their experiences of violence, sexual risk and substance use behaviors, as part of a study on the effectiveness of a family planning program in a state correctional facility; data from pretest surveys (N=447) were used for current analyses. Logistic regression analyses adjusted for demographics were used to assess significant associations between gender-based violence variables (i.e., adolescent intimate partner violence (IPV), adult IPV, childhood sexual assault (SA), adolescent SA, and adult SA) and arrests due to sex trade or drug-related charges. Significant relationships were observed between arrests for sex trade and adult SA (OR=2.1, 95%CI=1.2-3.6), adolescent IPV (OR=2.5, 95%CI=1.5-4.1), and adult IPV (OR=1.7, 95%CI=1.1-2.6); no significant associations were observed for drug-related charges. Findings from the current study demonstrate that experiences of gender-based violence are associated with arrests for sex trade but not drug-related charges. Interventions for incarcerated women are needed to consider and address history of victimization from gender-based violence and its relation to women's historic and future sex trade involvement.     

Factors affecting perceptions of medical errors

The purpose of the study was to assess how three factors affect subjects' perceptions of a medical error made in a hypothetical scenario. The factors were (i) type of compensation system, (ii) degree of procedural “voice” present in the physician-patient relationship, and (iii) magnitude of damage sustained by the patient. Perceptions were defined to include subjects' (i) emotional reactions to the incident, (ii) causal attributions about the incident, (iii) evaluations of the faimess of the compensation systems, and (iv) compensation demands. Results indicated that damage magnitude was the only factor that affected subjects' emotional reactions to the described incident and their compensation requests. When damage was severe, subjects reacted more negatively to the event and demanded greater compensation. Subjects rated the no-fault system and greater voice conditions as more fair. In general, subjects causally analyzed the error incident in a manner that was less blameworthy of the physician when procedural justice was high, especially if this was combined with a no-fault compensation system. Results were discussed in terms of Wexler and Winick's (1991) model of therapeutic jurisprudence.     

Intimate Partner Violence Screening in a Women Veterans’ Health Clinic: Assessing Feasibility

Women presenting for care within a suburban Department of Veterans Affairs Hospital (VA) were screened for intimate partner violence (IPV). This study aimed to explore the feasibility of screening for IPV within a VA women’s health clinic, assess how well the screening measure captured women veterans’ experiences of IPV, and compare clinical correlates of IPV in women veterans who have and have not experienced IPV. Of 96 eligible women, 93 (97 %) answered a self-report question regarding experience of lifetime IPV and 72 (75 %) participated in a standardized screening. Among participants, 42 (47 %) reported experiencing past or current IPV, and of those, 11 (25 %) reported that they were currently experiencing IPV, and 31 (70 %) reported that they had experienced IPV in their past. Screening for IPV among women veterans in a women’s health clinic is feasible and identifies women who experience IPV, offering opportunities for referral and intervention.     

Examining the Long-Term Consequences of Bullying on Adult Substance Use

Violence is a major concern in the school safety literature. With the potential negative impact of bullying victimization, it is imperative that bullying also be seen as an important social problem that has potential long-term mental and physical health consequences (CDC Surveillance Summaries, 65(6), 1-50, 2016). Numerous studies have documented the negative consequences of bullying in childhood, contending that bullying during childhood can lead to a variety of adulthood problems (Farrington, Loeber, Stallings, & Ttofi Journal of Aggression, Conflict and Peace Research, 3(2), 74-81, 2011; Rigby, 2007). Fewer studies have examined the long-term consequences of childhood bullying on adult substance use. This study uses Bureau of Labor Statistics’ National Longitudinal Survey of Youth (1997) data to examine the association between school-aged bullying (under the age of 19) and adult alcohol, cigarette, and drug use (using Wave 10 from 2007).