A more sensitive method for the quantitation of genomic DNA by Alu amplification

Current procedures for human DNA quantitation reach their limit at 150 pg DNA, which is above the limit of the PCR profiling range using Profiler-Plus (Applied Biosystems, CA). This study tested the potential for the use of primate specific Alu sequences in forensic science for the sensitive detection and quantitaion of DNA. A fluorescently labelled primer pair was designed enabling high efficiency amplification of the core Alu sequence within primate DNA. Quantitation was performed by measurement of fluorescence intensity and comparison to a series of standard template DNA amounts via the construction of a standard curve. The new Alu-based quantitation protocol developed has shown its feasibility in more sensitively quantitating (100-2.5 pg) unknown amounts of human DNA for forensic use. The method is compatible with the use and throughput of current forensic procedures.     

Age and Gender Differences in Peer Conflict

The purpose of this study was to examine age and gender differences in peer conflict, particularly in regards to conflict issues and resolution strategies reported by children and adolescents. Students from grades 4 and 8 (60 boys, 60 girls) were asked interview questions and given 3 hypothetical scenarios to respond to. Teacher and self-reports were also gathered for each student to assess their level of adaptive ability. The results showed that adolescents tended to report higher rates of conflict and endorsed more cooperative strategies than 4th graders, who endorsed more aggressive tactics. Female students reported having more relational issues and used more conflict-mitigating strategies, while boys reported having more conflicts related to status/dominance. Finally, links were also found between effective resolution strategies and social ability. The implications and limitations of this study are discussed.M. A. Noakes received her Master's degree from the Department of Educational Psychology at the University of Alberta, Canada. Her current research interests include assessment of at-risk youth, relapse prevention programs for young offenders, and social emotional functioning of children and adolescents.C. M. Rinaldi her doctorate from the Department of Educational and Counselling Psychology at McGill University, Canada. Her current research interests include applied developmental psychology, social emotional functioning of children and adolescents, and parent-child relations.     

About the Authors

Biography

About the Authors     

Interrogation of short tandem repeats using fluorescent probes and melting curve analysis: A step towards rapid DNA identity screening

Current forensic DNA profiling methods rely on the analysis of samples at specialised laboratories with an average turnaround time of several days. The ability to rapidly determine a partial profile of short tandem repeats at the point-of-arrest would be of great benefit to police forces around the world, for example enabling a suspect to be rapidly included or excluded from an investigation. We have developed a homogeneous PCR method for the interrogation of STR loci utilising fluorescent oligonucleotide probes and melting curve analysis. Alleles of the D18S51, TH01 and D8S1179 loci were differentiated and identified on the basis of target length and probe melting temperature. Assay performance was evaluated by comparing melting peak data with the AmpFlSTR^® SGM Plus^® system. The method is compatible with direct analysis of unpurified buccal swab samples, enabling a partial STR profile to be generated within 1 h.     

Groups Work for Women: Gender and Group Identity in Social Dilemmas

Behavior in social-dilemma (mixed-motive) situations has been of great interest to economists, psychologists, and negotiation scholars. In this study, we used a threshold social-dilemma game to examine factors that have not yet been investigated and that may have an impact on behavior in these settings: gender and group identity. We found that, for women, interacting with members of a naturally occurring group increased coordination and efficiency, while for men, interacting with members of a naturally occurring group decreased coordination and efficiency. Psychological literature on gender differences and group interdependence explains these differences. We conclude by discussing the implications of these results for gender differences in negotiation behavior.     

Democratization through European integration: The case of minority rights in the Czech Republic and Romania

While scholars have tended to focus on domestic factors as most critical to the consolidation of democracy, the post-communist European Union (EU) candidate states have exhibited a unique confluence of domestic and foreign policies, due to their objective of EU membership. This article assesses and compares the impact of the EU on policy making in two diverse candidate states in their first decade of transition, focusing on minority rights protection as a fundamental requirement of both EU membership and a stable democracy. I find that the EU has played a principal role in the reform process and democratic consolidation of candidate states, even in the controversial field of minority rights. The degree and nature of the EU’s impact, however, has depended in part on the activism of the particular minority, EU interest and pressure, EU Member States’ own domestic policies, and the persistence of racism in society. Dr. Melanie H. Ram is a research associate at the Institute for European, Russian and Eurasian Studies at George Washington University and Senior Program Officer for the Japan International Cooperation Agency USA Office. She has written extensively on European Union enlargement and democratic consolidation and reform in Central and Southeastern Europe, and is the author most recently of “Harmonizing Laws with the European Union: The Case of Intellectual Property Rights in the Czech Republic” inNorms and Nannies: The Impact of European Organizations on Central and East European States (2002). Earlier versions of this article were presented at the American Political Science Association Annual Meeting, 30 August–2 September 2001, San Francisco, CA and at “Voice or Exit: Comparative Perspectives on Ethnic Minorities in Twentieth Century Europe,” Humboldt University, Berlin, 14–16 June 2001.