Validation of male-specific, 12-locus fluorescent short tandem repeat (STR) multiplex

Y chromosome-specific short tandem repeat (Y-STR) analysis has become another widely accepted tool for human identification. The PowerPlex Y System is a fluorescent multiplex that includes the 12 loci: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. This panel of markers incorporates the 9-locus European minimal haplotype (EMH) loci recommended by the International Y-STR User Group and the 11-locus set recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Described here are inter-laboratory results from 17 developmental validation studies of the PowerPlex Y System and include the following results: (a) samples distributed between laboratories and commercial standards produced expected and reproducible haplotypes; (b) use of common amplification and detection instruments were successfully demonstrated; (c) full profiles were obtained with standard 30 and 32 cycle amplification protocols and cycle number (24-28 cycles) could be modified to match different substrates (such as direct amplification of FTA paper); (d) complete profiles were observed with reaction volumes from 6.25 to 50 microL; (e) minimal impact was observed with variation of enzyme concentration; (f) full haplotypes were observed with 0.5-2x primer concentrations; however, relative yield between loci varied with concentration; (g) reduction of magnesium to 1mM (1.5 mM standard) resulted in minimal amplification, while only partial loss of yield was observed with 1.25 mM magnesium; (h) decreasing the annealing temperature by 2-4 degrees C did not generate artifacts or locus dropout and most laboratories observed full amplification with the annealing temperature increased by 2 degrees C and significant locus dropout with a 4 degrees C increase in annealing temperature; (i) amplification of individual loci with primers used in the multiplex produced the same alleles as observed with the multiplex amplification; (j) all laboratories observed full amplification with >or = 125 pg of male template with partial and/or complete profiles observed using 30-62.5 pg of DNA; (k) analysis of < or = 500 ng of female DNA did not yield amplification products; (l) the minor male component of a male/female mixture was observed with < or =1200-fold excess female DNA with the majority of alleles still observed with 10,000-fold excess female; (m) male/male mixtures produced full profiles from the minor contributor with 10-20-fold excess of the major contributor; (n) average stutter for each locus; (o) precision of sizing were determined; (p) human-specificity studies displayed amplification products only with some primate samples; and (q) reanalysis of 102 non-probative casework samples from 65 cases produced results consistent with original findings and in some instances additional identification of a minor male contributor to a male/female mixture was obtained. In general, the PowerPlex Y System was shown to have the sensitivity, specificity and reliability required for forensic DNA analysis.     

``Is Alec a Rapist?' – Cultural Connotations of `Rape' and `Seduction' – A Reply to Professor John Sutherland

This article is a response to an essay written by an academic in English Literature, Professor John Sutherland. Through close textual analysis,Sutherland purports to resolve a well-known literary question: whether the sexual encounter outlined in the Victorian novel Tess of the d'Urbervilles should be classified as rape or seduction. The present article rejects his conclusion on the matter. An(equally) close analysis of the fictional text in question and of Sutherland's gloss, demonstrates the partiality of his critique, both in literary-critical and critical-legal terms. In addition, examination of the conceptual and historico-legal context regarding the notions of rape and seduction on both sides of the Atlantic highlights parallels between Sutherland's own partiality and that of the law. In short, the apparent objectivity of the textual analysis and subsequent critique undertaken by Sutherland is revealed as a continuation of legal and patriarchal prejudices defining rape and seduction. The use of close textual analysis as the key critical device promotes the apparent probity of his findings. Locating them in an essay collection designed for mass lay public consumption completes the circle – from partisan scholarship to `informed' popular prejudice. This revised version was published online in July 2006 with corrections to the Cover Date.     

Development of a one-tube extraction and amplification method for DNA analysis of sperm and epithelial cells recovered from forensic samples by laser microdissection

Laser microdissection can be used in forensic casework to isolate specific cell types from mixtures of biological samples. Extraction of DNA from selected cells is still required prior to STR amplification. Because of the relatively pristine nature of the recovered cells, laser microdissection is more sensitive than more traditional methods of DNA analysis, theoretically resulting in DNA profiles from less cellular material. A one-tube extraction and amplification method minimises loss of DNA through liquid transfers and reduces the potential for contamination events occurring. In this paper, the development of a one-tube method for the effective extraction of DNA from laser microdissected sperm and epithelial cells is described. The performance of the in-house method was compared to that of a commercial DNA extraction kit for extraction of DNA from sperm and the downstream compatibility with STR amplification was determined for both sperm and epithelial samples. Full Identifiler™ profiles after 28 amplification cycles were obtained from as few as 15 epithelial cells and 30 sperm.     

The effect of incomplete information in a threshold public goods experiment

Fiscal stress and decreasing government budgets have led to renewed interest in voluntary contributions for the funding of public goods. This paper experimentally examines the Provision Point Mechanism (PPM), a voluntary contribution mechanism for the funding of lumpy public goods. Previous research has demonstrated the effectiveness of this mechanism at providing public goods, however all were conducted in an environment of complete information, which fails to capture the uncertainties of the real world. This study tests the efficacy of the PPM in informationally limited settings. We find no significant differences in the rate of successful provisions or level of group contributions when subjects have limited information about the valuations of others than when they have complete information.     

The 2009 Landtag Election in Saxony

The Landtag election in Saxony was one of three elections for state parliaments, all taking place on 30 August 2009. Out of the three, it was the one where there was little doubt that the CDU would, once again, appoint the next Prime Minister. Aside from the lack of surprise in the election outcome, this election had some historic moments. After the right-wing NPD was able to re-enter the Saxon Landtag, Saxony is the only German state parliament with six party parliamentary groups present. Further, turnout at this election was 52 per cent, an all-time low. The election ended Saxony's grand coalition and established its first CDU–FDP coalition. Within two weeks after the election, the new coalition contract was negotiated and the new government was sworn in so as to present a clear signal to national voters that a black and yellow coalition was a good and workable alternative to the grand coalition in the German Bundestag as well.     

Whole-face procedures for recovering facial images from memory

Research has indicated that traditional methods for accessing facial memories usually yield unidentifiable images. Recent research, however, has made important improvements in this area to the witness interview, method used for constructing the face and recognition of finished composites. Here, we investigated whether three of these improvements would produce even-more recognisable images when used in conjunction with each other. The techniques are holistic in nature: they involve processes which operate on an entire face. Forty participants first inspected an unfamiliar target face. Nominally 24 h later, they were interviewed using a standard type of cognitive interview (CI) to recall the appearance of the target, or an enhanced ‘holistic’ interview where the CI was followed by procedures for focussing on the target's character. Participants then constructed a composite using EvoFIT, a recognition-type system that requires repeatedly selecting items from face arrays, with ‘breeding’, to ‘evolve’ a composite. They either saw faces in these arrays with blurred external features, or an enhanced method where these faces were presented with masked external features. Then, further participants attempted to name the composites, first by looking at the face front-on, the normal method, and then for a second time by looking at the face side-on, which research demonstrates facilitates recognition. All techniques improved correct naming on their own, but together promoted highly-recognisable composites with mean naming at 74% correct. The implication is that these techniques, if used together by practitioners, should substantially increase the detection of suspects using this forensic method of person identification.     

The liberal character of ethological governance

This paper examines the liberal government of ‘character’ from the perspective offered by those practices, strategies and techniques I term ‘ethological governance’. Ethological governance is neither an ideology nor a tradition of thought, but denotes an orientation to human conduct that is organized by an explicit concern with character and its formation. The paper argues that ethological governance, especially in its Anglo-American strain, subscribes to a developmental notion of human conduct (i.e. character) and serves as a standard for liberal government by judging the responsible exercise of freedom. To this end, the paper examines how ethological governance establishes a context of government that harnesses character as a tool for social and political transformation in the nineteenth and early twentieth centuries by (1) explaining how character establishes a normative scale against which the capacity for individuals to practise their freedom is measured and (2) offering a symptomatic reading of those aspects of John Stuart Mill's work that deal with ethology in order to show how character indexes the judicious limits of government and serves as a basic test of citizenly competence.