DNA Preparation from Sexual Assault Cases by Selective Degradation of Contaminating DNA from the Victim

Abstract:  The standard method to purify sperm DNA from vaginal swabs taken from rape victims is to selectively digest the victim's epithelial cells to solubilize the victim's DNA, and then separate the soluble DNA from the intact sperm by centrifugation. A different approach to removing the soluble victim's DNA is to selectively degrade it using a nuclease, DNase I. DNase I reduces the amount of soluble DNA by over 1000-fold, while having virtually no effect on the sperm DNA remaining in the sperm head and inaccessible to the enzyme. Nuclease inactivation and sperm lysis then yield a soluble, pure male DNA fraction. An aliquot of soluble DNA is removed prior to nuclease addition to provide the victim's fraction. Vaginal swabs taken at defined time points following consensual sex and taken from rape victims were processed using the nuclease method or the standard method and the nuclease method gave superior short tandem repeat profiles.     

Cognitive Dissonance Resolution Strategies After Exposure to Corporate Violence Scenarios

This study uses cognitive dissonance as a theoretical avenue to identify factors that might hinder the public’s acceptance of incontrovertible facts about corporate violence (i.e., the calculated endangerment of civilians, workers, and customers). Three hundred and twenty-seven participants answered a survey that measured their (1) support for capitalism, (2) level of nationalism, (3) socio-demographic characteristics, and (3) attitudes toward three scenarios describing corporate violence cases. These attitudes comprised participants’ (a) acceptance of the scenarios as true, (b) perceived seriousness of the cases presented to them, and (c) support for several statements made to justify the companies’ actions. Results of statistical analyses suggest that subjects who scored higher on the nationalism and pro-capitalism scales were less likely to rate the cases as serious, and more inclined to rationalize the corporations’ actions. These findings imply that myth adherence might lead business supporters and nationalists to reject inconvenient truths relative to crimes of the powerful, which would then undermine the effect of increased awareness on prosecutorial efforts against corporate crime in the U.S.     

欧洲宪法司法的多样性——兼及法国之例外

目次一、各国宪法司法中不同的抽象审查程度二、各国宪法司法的集中程度三、结论所谓宪法司法,即对国家所有行为包括立法行为、行政行为或司法行为等进行违宪司法审查。欧洲的宪法司法萌芽于19世纪下半叶。但无可否认的是,仅在诞生后的一个世纪,宪法司法方在欧洲得到显著发展。     

Reconstruction of the state: Lobbying against political corruption in the Czech Republic

High level of distrust in political elites accompanied with a strong perception of corruption in Czech Republic has led to an increase of several anti‐corruption nongovernmental organisations' activities. About 18 organisations have created in 2013 an open coalition and established a project called “Reconstruction of the State” (Rekonstrukce státu). This project is oriented towards political parties and political elites to lead them to adopt nine anti‐corruption legislative proposals. This paper analyses how the project operates in its formal and informal aspects, which communication means are preferred and how the lobbyists interact with the politicians and political parties. Apart from the analysis of publicly available pieces of information from the project's website and official printed materials, semistructured interviews with activists and field research were performed. It can be seen that Reconstruction of the state has been most successful regarding agenda‐setting, but in the context of the legislative process, it has to face many obstacles either from legislators themselves or competitors with different goals.     

The architecture of organizations as missed opportunity in political research

Few scholars in political science or public administration deny that organization matters. However, the rare application of an organizational perspective in research suggests otherwise. To revive the awareness of organizations as unit or level of analysis, attributed and generic properties are distinguished. Attributed properties, which dominate research, assign functions and patterns of behaviour to an organization. In contrast, generic properties refer to the constitutive elements of organizations that take effect before attributed properties. This article takes a closer look at four generic properties by examining their often-implicit use in current political science and public administration research. The aim is to demonstrate that the formal dimension, the goals, the expertise of personnel and organizational boundaries exert an independent influence on the output of political or public sector organizations.     

Economic Crises,Crisis of Labour Law? Lessons from Weimar

Labour law has been thrown into turmoil in many large industrialized countries with democratic tradition and market economies. In fact, rapid economic globalization resulted in an irremediable decline in collective bargaining in most of the states that entered into the sphere of Anglo-Saxon capitalism. On a first reading, the financial crisis of 2008 exacerbated this retreat of labour law back to its initial individualist and contractual forms. In analysing the contemporary crisis of labour law, the historical-comparative method can be highly fruitful, especially if one considers the precedent of the economic crisis of 1929. On this basis, I first consider an influential text by Hugo Sinzheimer on the ‘crisis of labour law’ in Weimar Germany and a study by Otto Kahn-Freund on the changing function of labour law in the same context. These works can be connected to the concept of the ‘labour constitution’, which Max Weber notably developed in an empirical sense, and which finds its extension in the later works of Thilo Ramm, a labour scholar of international reputation.     

Democracy, Sea Power and Institutional Change: An Economic Analysis of the Athenian Naval Law

The present essay attempts to test the validity of the theory of institutional change based on the concept of property rights, in the remote historical setting of Classical Athens. The Naval Law introduced by Themistocles in 483-2 BC, established the institution of trierarchy to produce warships to face the Persian invaders. This reform led to the first attempt to define the costs and benefits of public goods in the field of national defense. Thus, institutional change in Ancient Athens, through an efficient arrangement of property rights and duties, had long-term consequences on economic performance, creating a new growth path and ensuring economic prosperity and social justice for more than 150 years.     

Maltreatment of handicapped children: A critical review

Despite increased interest in child abuse and neglect in the past decade, little research attention has been focused on maltreatment in handicapped populations. This is surprising given that many handicapped children display characteristics (e.g., chronic and pervasive behavioral disturbances, decreased social initiations with caregivers) that are associated with high risk for assault and neglect in nonhandicapped children. Numerous investigations have found an overrepresentation of handicapped children in maltreated samples, and results of more recent efforts reveal a high incidence of abuse and neglect in handicapped populations. Although these data underscore the high risk for maltreatment in handicapped children, a number of important issues warrant further empirical examination. Moreover, generalizations and interpretations of findings from previous research have been obfuscated by methodological shortcomings. The present paper reviews the literature concerning abuse and neglect of handicapped children. Research strategies and issues are delineated and current problems in this field are discussed. Suggestions for directions future research might take are offered.     

Successful extraction of human genomic DNA from serum and its application to forensic identification

We report here on the successful extraction of human genomic DNA from a serum sample in a forensic case. The extracted DNA was successfully used for the identification of remains presumably immersed for more than three weeks for which the only comparison sample was a 250-microL serum aliquot kept frozen in a laboratory. The analysis made it possible to identify a second victim as the daughter of the first.     

A case study of the suicide of Luis Mendès France, in Bordeaux, in 1695

After being condemned and imprisoned in Lisbon by the Portuguese Inquisition, Luis Mendes de Franca exiled himself in late 1683 to France, where his descendants adopted the family name Mendes France. In 1695, at the age of 55 years, Luis Mendes committed suicide in Bordeaux by a pistol shot that decapitated him. The inquest conducted at that time concluded that Luis Mendes was insane and thus not guilty of the crime of suicide. We hypothesize that he used a flint stone-type pistol loaded with an extraordinarily large quantity of black gunpowder. Using available information on historic firearms, ammunition, and powder, coupled with the preserved testimony of historic figures, we propose a reconstruction of this drama and a diagnostic approach to the psychiatric aspects of the suicide.