Characterization of human control region sequences of the African American SWGDAM forensic mtDNA data set

The scientific working group on DNA analysis Methods (SWGDAM) mitochondrial DNA (mtDNA) population data set is used to infer the relative rarity of control region mtDNA profiles obtained from evidence samples and of profiles used for identification of missing persons. In this study, the African American haplogroup patterns in the SWGDAM data were analyzed in a phylogenetic context to determine relevant single nucleotide polymorphisms (SNPs) and to describe haplogroup distributions for Africans observed in these data sets. Over 200 SNPs (n=217) were observed in the African American data set (n=1148). These SNPs ranged from having 1-39 changes in the phylogenetic tree, with sites 152 and 16519 being the most variable. On average there were 5.8 changes for a character on the tree. The most variable sites (with 19 or more changes each) observed included 16093, 16129, 16189, 16311, 16362, 16519, 146, 150, 152, 189, and 195. These rapidly changing sites are consistent with other published analyses. Only 34 SNPs are needed to identify all clusters containing 10 or more individuals in the African American data set. The results show that the African American SWGDAM mtDNA data set contains variation consistent with that described in continental African populations. Thirteen of the 18 haplogroups previously observed in African populations were observed and include: L1a, L1b, L1c, L2a, L2b, L2c, L3b, L3d, L3e1, L3e2, L3e3, L3e4 and L3f. Haplogroup L2a is the most commonly observed cluster (18.8%) in the African American data set. The next most common haplogroups in the African American data set include the clusters L1c (11.0%), L1b (9.1%), L3e2 (9.0%) and L3b (8.1%). Approximately 8% of the haplogroups observed within African Americans were common in European Caucasians or East Asians; these were H (n=32), J (n=4), K (n=5), T (n=2), U5 (n=6), U6 (n=9 also known from North Africa), A (n=12), B (n=7), C (n=4), and M (n=16), respectively. The European Caucasian and East Asian haplogroups are expected due to admixture between individuals with recent ancestry in Western Eurasia and sub-Saharan Africa. The genetic characterization of these relevant data sets is fully consistent with other published mtDNA genetic variation. The sequence diversity observed in this data set makes it a valuable tool for forensic applications.     

A novel method of automated skull registration for forensic facial approximation

Modern forensic facial reconstruction techniques are based on an understanding of skeletal variation and tissue depths. These techniques rely upon a skilled practitioner interpreting limited data. To (i) increase the amount of data available and (ii) lessen the subjective interpretation, we use medical imaging and statistical techniques. We introduce a software tool, reality enhancement/facial approximation by computational estimation (RE/FACE) for computer-based forensic facial reconstruction. The tool applies innovative computer-based techniques to a database of human head computed tomography (CT) scans in order to derive a statistical approximation of the soft tissue structure of a questioned skull. A core component of this tool is an algorithm for removing the variation in facial structure due to skeletal variation. This method uses models derived from the CT scans and does not require manual measurement or placement of landmarks. It does not require tissue-depth tables, can be tailored to specific racial categories by adding CT scans, and removes much of the subjectivity of manual reconstructions.     

Overcoming barriers to technology transfer between small business assistance agencies and their clients: Third world style

This paper presents the results of an empirical examination of the methods small business assistance agencies in the Dominican Republic use to overcome technology transfer barriers. The availability and the methods agencies use to access the world's business knowledge are examined, as well as how the agencies disseminate the information to clients. The discussion identifies barriers that inhibit the flow of client information between (1) the knowledge pool and the agencies and (2) the agencies and the client. The strategies that agencies use to overcome barriers are characterized as push or pull, and informal or formal. Pull strategies begin with marketplace need and work toward the technology to solve the problem. Push strategies begin with specific business information and work toward its acceptance and use in the marketplace by clients. Personal interviews were conducted in Spanish with heads of the 13 assistance agencies in the country. Survey participants were asked about the processes, procedures, and techniques they used to gain and transfer business skills. The study identifies specific activities in which the agencies engage in order to effectively overcome barriers to the transfer of business knowledge.     

The Burger Court's View of the Relationship Between the States and Their Municipalities

This article examines selected Burger Court cases concerningmunicipal antitrust immunity in order to determine whether theU.S. Supreme Court has a theoretical perspective on the relationshipbetween the states and their municipalities. After delineatingthe constitutional position of municipalities through an examinationof key Court cases and Dillon's rule, the study explores thederivative status of state-action immunity to municipalitiesin antitrust suits. In two significant cases, including oneinvolving a home rule city, the Burger Court has refused togrant municipalities the state-action exemption granted statesunder the Sherman Act. But, in reinforcing the unitary relationshipbetween the states and their municipalities, the Court has notaddressed Dillon's rule or the ambiguities inherent in denyingmunicipalities the residual powers of local self-government.     

Should forensic patients be informed of evaluators' opinions prior to trial?

There are several articles in the literature that discuss the problems which occur when persons who have been evaluated by forensic clinicians hear the results of those evaluations for the first time in court. The authors agree that the scenarios presented are problematic but suggest that in many cases the problems can be avoided by sharing the information with the person prior to presenting it in court. They present several case examples to illustrate their point.