The use of cyclohexanone as a "derivatizing" reagent for the GC-MS detection of amphetamines and ephedrines in seizures and the urine

A GC-MS method has been developed for the detection of amphetamine, methamphetamine, and the ephedrines, in seizures and the urine, based on on-GC condensation (derivatization) with cyclohexanone. The method is simple: the dried seizure material or the urine extract was mixed with cyclohexanone and injected into the GC-MS. The method was found to be superior to the methods based on acyl and trimethylsilyl (TMS) derivatization. Unlike for the acyl and TMS derivatives, the molecular and fragment ions of the cyclohexanone condensation products (cyclohexanone derivatives) were of substantial abundance, a useful property in unambiguous compound characterization. Furthermore, the high stability of the "derivatizing" reagent, cyclohexanone, compared with acyl and TMS derivatizing reagents, is a useful property in method development. The present method has proved selective and, tentatively, sensitive enough in the following areas (where methods based on acyl and TMS derivatization, as tested in this laboratory, have failed): (a) detection of amphetamine as a metabolite of methamphetamine; (b) detection of norpseudoephedrine as a metabolite of pseudoephedrine; (c) detection of amphetamine as an impurity of methamphetamine; (d) detection of cathine (norephedrine) as a constituent of Khat leaves; and (e) differentiation of Khat use from phenylpropanolamine use.     

Congenital atrioventricular block: histological aspects

It is known that maternal immunological factors such as systemic disease are involved in the genesis of cardiac conduction problems in the fetus but the histologic changes in the conduction system are less documented. We report the case of a 33-year-old woman with no significant medical history. Her first pregnancy was induced by Clomifene. At 17 weeks of gestation, the fetus presented sonographic abnormalities characteristic of a complete atrioventricular block. Biological investigations found anti-SSA and -SSB antibodies. Clinical history search for systemic disease was positive for photosensitivity, lasting 10 years, suggesting the diagnosis of systemic lupus erythematosus. The patient was treated with prednisone 20 mg per day but fetal death occurred at 29 weeks of gestation. Histological examination of the fetal heart showed an altered atrioventricular node and bundle of His with fibrosis, calcifications, endocardial fibroelastosis and mononucleated inflammatory cells. The search for these specific lesions can be determinant in establishing the disease pathogenesis; also, it is important to eliminate this diagnosis in an unexplained fetal death.     

La planification stratégique à Ottawa et à Québec: une comparaison de quelques ministères

Sommaire: Douze ministères (six à Ottawa et six à Québec) ont fait l'objet d'une recherche visant àétudier le processus de planification stratégique. Cette planification vise d'abord à identifier les enjeux stratégiques et à déterminer les orientations d'un ministère. Elle vise aussi, dans plusieurs ministères étudiés, à introduire un changement culturel, à mieux intéger les diverses parties de l'organisation, à améliorer l'efficacité et la productivité, à accroître la satisfaction et la qualité de vie au travail, ainsi qu'à améliorer les services à la clientèle et les communications. Au niveau de la gestion du processus, les auteurs ont constaté de grandes différences entre les ministères québécois mais une certaine homogénéité au niveau des ministères fédéraux où le processus semble davantage institutionnalisé. Par ailleurs, à Québec, les plans stratégiques donnent lieu à la préparation de plans annuels d'action élaborés pour l'ensemble du ministère, alors qu'à Ottawa, la responsabilité de la mise en application est laissée aux responsables de secteurs. Enfin, les auteurs ont identifié certaines conditions de succès: l'implication personnelle du sousministre et de l'équipe de direction, l'appui du ministre, la stabilité de la direction, un exercice conçu sur mesure, la simplicité du processus, la capacité de gérer les conflits, la volonté de régler les problèmes urgents sans attendre la fin du processus, la consultation et la responsabilisation des gestionnaires et, enfin, l'intégration de la planification stratégique au processus de prise de décision central du ministère. Abstract: This study designed to analyse the strategic planning process covered twelve departments (six in Ottawa and six in Quebec City). This planning effort is designed primarily to identify the strategic stakes and to determine the orientation of a department. In several departments studied, it also aims to introduce cultural change, to better integrate the various components of the organization, to improve efficiency and productivity, and to raise work satisfaction and the quality of working life, thus improving client services as well as communications. Regarding the management of the process, the authors found major differences between Quebec departments, but a certain homogeneity among federal departments, where the process seems more institutionalized. Moreover, in Quebec, strategic plans involve the preparation of annual action plans developed for an entire department, whereas in Ottawa responsibility for implementation is left to sector heads. Finally, the authors identify certain conditions for success: the personal involvement of the deputy minister and the management team, the minister's support, management stability, a custom-designed exercise, the simplicity of the process, the ability to manage conflict, a willingness to solve urgent problems without waiting for the end of the process, consultation and accountability of managers and, finally, integration of strategic planning into the department's central decision-making process.     

Quantification of mitochondrial DNA in human blood cells using an automated detection system

The 4977 bp deletion of mitochondrial DNA (mtDNA) accumulates in postmitotic tissues with advancing age. The purpose of our study was to detect and quantify these deletion even in blood cells with a high turnover activity. Whole venous blood, isolated human platelets and peripheral blood mononuclear cells (PBMCs) were collected from 10 unrelated donors aged 20-71 years and total DNA was extracted. PCR was performed for total and mutated mtDNA using two different primer pairs and two fluorogenic probes labeled with the fluorescent dyes FAM and VIC. Specific PCR products were generated, detected and quantified in a real-time PCR. The amplification products of total and deleted mtDNA could be detected in each sample and did not exhibit any differences in the amount of the deleted mtDNA in whole blood, human platelets or PBMCs. Our data did not show any accumulation of the 4977 bp deletion with increasing age as it was observed for several other tissues.     

A Study of the Presence of Gunshot Residue in Pittsburgh Police Stations using SEM/EDS and LC‐MS/MS

Due to possible secondary transfer of gunshot residue (GSR) onto a suspect in police custody prior to sampling, a baseline must be created for the amount of GSR present. With an increase of “lead free” ammunition, testing for both gunpowder and primer GSR is relevant. Seventy samples were collected using carbon‐coated adhesive stubs from four Pittsburgh Police Stations and vehicles to investigate these locations as sources of secondary GSR contamination. These seventy samples were analyzed for primer GSR using scanning electron microscopy‐energy‐dispersive X‐ray spectrometry. One primer GSR particle was detected; no sample was classified as positive for primer GSR. These same samples were then analyzed for gunpowder GSR using liquid chromatography coupled to tandem mass spectrometry to test for akardite II, ethylcentralite, diphenylamine, N‐nitrosodiphenylamine, 2‐nitrodiphenylamine, and 4‐nitrodiphenylamine. Ethylcentralite was quantifiable in two test samples. These results suggest there is a negligible potential for secondary transfer of primer and gunpowder GSR.     

Police Personnel Affective Profiles: Differences in Perceptions of the Work Climate and Motivation

The affective profile model was used to investigate individual differences in police personnel perceptions about the working climate and its influences on motivation. The Positive Affect, Negative Affect Schedule (PANAS) was used to assign police personnel, sworn and non-sworn (N = 595), to four affective profiles: self-fulfilling, low affective, high affective, and self-destructive. The work climate was assessed using the Learning Climate Questionnaire (Management Relations and Style, Time, Autonomy and Responsibility, Team Style, Opportunities to Develop, Guidelines on How to do the Job, and Contentedness). Motivation was evaluated using a modified version (to refer specifically to the individual’s work situation) of the Situational Motivation Scale (intrinsic motivation, external regulation, identified regulation, and amotivation). Self-fulfilling individuals scored higher on all work climate dimensions compared to the other three groups. Compared to low positive affect profiles, individuals with profiles of high positive affect scored higher in intrinsic motivation and identified regulation. Self-destructive individuals scored higher in amotivation. Different aspects of the work climate were related to each motivation dimension among affective profiles. Police personnel may react to their work environment depending on their affective profile. Moreover, the extent to which the work influences police personnel’s motivation is also related to the affective profile of the individual.     

Examining the Interplay of Justice Perceptions,Motivation, and School Achievement among Secondary School Students

There is a paucity of empirical research on the social psychology of justice in educational settings. A few previous studies have predominantly focused on distributive and procedural justice concerns, and knowledge about the role of what have been called informational and interpersonal justice for school outcomes is very scarce. In the present study, data from 227 eighth- and ninth-grade students who participated in a survey study were analyzed to examine the interplay between relational justice concerns (decomposed into procedural, interpersonal, and informational justice), motivation to study, and school achievement. A comprehensive theoretically grounded multi-item measure of informational justice was developed and validated. The results showed that informational justice significantly predicts school grades, and that motivation to study fully mediates this effect. Neither procedural nor interpersonal justice was associated with school grades. The implications of these results for research and practice are discussed in detail.     

Fatal rupture of hepatic adenomatosis: Autopsy case and review of the literature

Hepatic adenomatosis is a rare disease consisting of multiple adenomas in otherwise-normal liver parenchyma. Though the discovery of this entity goes back several years, its diagnosis is still challenging in terms of its definition and pathophysiology. Clinically, patients may be completely asymptomatic and the diagnosis is only made incidentally through imaging tests. The discovery could be made when complications occur such as intraperitoneal hemorrhage with hypovolemic shock due to the rupture of an adenoma. We report a fatal case of a ruptured adenoma in a case of hepatic adenomatosis discovered at autopsy. In order to achieve a better view of this disease, we conducted a literature review on this subject describing the pathogenesis, manifestations, and autopsy contribution to addressing this entity.     

Terrorism in India as a Determinant of Terrorism in Pakistan

Terrorism in Pakistan springs from many factors such as, poverty, corruption, inequality, sham democracy, and low-level literacy; however, the effect of terrorism in India over terrorism in Pakistan remains unnoticed. This study explores the role of terrorism in India as a determinant of terrorism in Pakistan. This fangled determinant of terrorism in Pakistan forms the premise of our work. The Johansen cointegration analysis technique confirms the long-run relationship among terrorism in India and Pakistan and unemployment in Pakistan. Error correction model (ECM) resulted 55% of convergence annually. Besides, ECM verifies both short and long-run causalities. The VECM Granger and Modified Wald (MWALD) causality tests reveal that terrorism in India and unemployment in Pakistan Granger causes terrorism in Pakistan, but no reverse causality exists. The study concludes that both terrorism in India and unemployment in Pakistan results in terrorism in Pakistan. Coping with terrorism, Pakistan and India must revisit their respective roles; and simultaneously, Pakistan should address the unemployment problem—which facilitates recruitment for the terrorist organizations.