A New Approach to the Investigation of Sexual Offenses—Cytoskeleton Analysis Reveals the Origin of Cells Found on Forensic Swabs*

Abstract: There are forensic inquiries in which an identification of epithelial cell types would provide important probative evidence. In cancer diagnosis, this information is yielded by histological examination of cytokeratin (Ck). Therefore, we tested 19 antibodies against different Cks (Ck1, Ck2e, Ck4, Ck5‐6, Ck7, Ck8, Ck9, CK10, Ck13, Ck14, Ck15, Ck16, Ck17, Ck18, Ck19, Ck20, Ck903, PanCkAE1_3, and CAM5‐2) on histological sections of epidermis, buccal mucosa, vaginal mucosa, penis, urogenital tract, and rectum and could identify two antigens unique to buccal‐cell and vaginal‐cell (Ck4) and skin epithelial‐cell (Ck10) cytokeratin. Subsequently, we developed an immunocytological technique for distinguishing swabbed skin and mucosal cells up to at least 1 year after sampling. By the detection of the Ck4 and Ck10 mRNAs in biopsy and laser capture microdissection collected samples via quantitative real‐time polymerase chain reaction, we were able to confirm our immunological findings. Hence, this study offers techniques to discriminate between skin and mucosal cells (buccal and vaginal) in forensic casework.     

Skin changes following defibrillation. The effect of high voltage direct current

Previously, electrical injuries have been suggested caused only by the concomitant heat developed during the passage of an electrical current. Recent experimental studies on fully anesthetized pigs and the study of one human case have, however, shown typical electrical alterations. The purpose of the present study was further to evaluate the histology of electrically induced changes in the skin in humans. In addition, supplementary in vivo methods for evaluation of skin changes as high-frequency ultrasound and Raman spectroscopy were used. The skin of 11 patients treated with a defibrillation of the heart was examined for macroscopic changes, the skin of eight of them also for histologic changes and for changes observable via supplementary methods. Immediately and 7 days after the defibrillation, fractions of a narrow red ring were observed along the periphery of the tin-foil electrode. Epidermis showed signs previously observed following electrical influence: segmental alterations often related to the openings of sweat ducts, darkstaining or "empty" nuclei and homogeneous cytoplasm, eosinophilic or pale. Dermis did not show the specific sign of electrical influence: deposits of calcium salts on dermal fibres, neither via histologic examination nor via high-frequency ultrasonography and Raman spectroscopy. Fractions of a narrow red ring along the periphery of the electrode showing histological signs of electric influence in epidermis thus appear to be characteristic of high voltage electrical injury.     

The Responsible Party Government Model in House and Senate Elections

Responsible party government theory requires that voters hold parties electorally accountable for their performance in control of government. Existing literature suggests that voters do this only to a limited extent—holding the presidential party's candidates responsible for government performance on Election Day. While this method of voting may hold the executive accountable for his performance, it is not really an effective way to hold the party in control of Congress accountable for its performance. The method falls short particularly when Congress is controlled by a different party than the president, but also whenever a Congress controlled by the same party pursues policies different from the president's. Using surveys of voters leaving the polls in the 1990, 1994, and 1998 midterm congressional elections, this study tests whether voters' evaluations of Congress's job performance also affect their support for majority party candidates in House and Senate elections, during both unified and divided government .     

Extreme Pessimists? Expected Socioeconomic Downward Mobility and the Political Attitudes of Young Adults

Political Behavior - In recent decades, and especially since the economic crisis, young people have been finding it more difficult to maintain or exceed the living standards of their parents. As a...     

Think Tank Involvement in Foreign Policymaking in the Czech Republic and Poland

Studies on foreign policy think tanks have too often remained disconnected from the analysis of foreign policy outcomes. Yet, investigating the development, functions and influence of think tanks can provide valuable insights into the context in which foreign policy is formulated. The Czech Republic and Poland represent interesting comparative cases in this regard: while Polish think tanks are more numerous and tend to be better placed in international rankings, they are less involved in the policymaking process than their Czech counterparts. This contrast has mainly to do with the sociology of foreign policy elites and the role of political parties in both countries.     

Potential Health Outcome and Vulnerability Indicators of Climate Change for Australia: Evidence for Policy Development

There is a growing need to develop health‐related indicators for climate change to assist in policy, planning, and evaluation of preventive measures. To date, no environmental health indicators of climate change have been developed specifically for Australia. We conducted a review of the Australian literature relevant to climate change health impacts to find out which exposure–response relationships could be readily used as indicators. The bulk of the literature relates to direct health‐related outcomes of extreme heat, and indirect outcomes associated with air pollution and infectious disease agents. Based on this information, evidence‐based indicators were chosen using the modified Driving force‐Pressure‐State‐Exposure‐Effect‐Action framework. Three groups of health outcome indicators are proposed: direct heat related, air pollution related, and climate‐sensitive infectious diseases. Indicators of human vulnerability to these outcomes are also included. The potential usefulness of and barriers to their use are discussed in the context of relevance for policy makers.     

Trafficking in human beings for labour exploitation. The case of the Netherlands

Exploitation of women in the sex industry is the best known but probably not the most common outcome of trafficking in human beings. Still, trafficking for labour exploitation gets less attention from policy makers, the police and researchers. The legal framework allows dealing with trafficking for labour exploitation, but the prosecution of such cases is still in its infancy. In this contribution we focus on labour exploitation. We first present the legal framework. Then we go into the lack of knowledge in this area, followed by what we do know about risk sectors, victims, and what makes them vulnerable for exploitation. Focussing on the Netherlands, we describe the perpetrators and the difficulties in investigating and prosecuting trafficing cases in general and labour exploitation in particular, rounding off with some general conclusions.