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421.
More than 20 years of economic reforms and opening up to the outside world have produced meaningful social, economic and political transformations in China. Have there been corresponding changes in the political–cultural orientations1 ‘Orientations’ here and thereafter refer to a broad range of beliefs, values, and assumptions that people hold about social and political life. Such orientations may be cognitive, affective, or evaluative. They are general in the sense that they may structure more specific attitudes or opinions. *?Yanlai Wang is a research associate at the Euro–Asia Centre; Nicholas Rees is Jean Monnet Professor of European Institutions and External Relations; and Bernadette Andreosso‐O'Callaghan is Jean Monnet Professor of Economics and Director, Euro–Asia Centre, all at the University of Limerick, Ireland. We would like to thank the University of Limerick for funding the 2000 survey. We would also like to thank Professor Andrew J. Nathan and Professor Tianjian Shi for the use of their survey questionnaire, Gary O'Brien and the two anonymous referees for their comments on the paper. View all notes of the Chinese public? This article examines some changes in orientations, based on the preliminary findings of an opinion survey conducted in China in 2000. The 2000 survey used an adapted form of a questionnaire used in a survey conducted in 1990 by Nathan and Shi. The evidence of the 2000 survey data suggests that the political–cultural orientations of the Chinese public are becoming more liberal and pro‐democratic as China's economy continues to grow, possibly providing the basis for a transition to some form of popular democracy.  相似文献   
422.
Examination of stomach contents is one of the important steps in medical legal autopsy. Vegetative materials such as stems, roots, and seeds in stomach contents can be valuable evidence for providing investigative leads in death investigation. Currently, the identification of plant materials relies on microscopic and morphologic examination. We have found that many seeds are often protected from acid degradation during stomach digestion by their tough exterior seed coat. Tomato seeds were selected as a model system to assess DNA analysis and plant variety marker identification. The DNA-amplified fragment length polymorphism method was performed to determine if the DNA obtained from single seeds could be used for PCR analysis. From the amplified fragment length polymorphism results, some candidate markers for individualizing seeds from morphologically distinct tomatoes were identified. These data on DNA analysis of tomato seeds indicate amplified fragment length polymorphism is a viable procedure for the individualization of seeds from stomach contents in forensic investigations.  相似文献   
423.
The use of a QIAamp DNA Stool Mini Kit (QIAGEN) for extracting human nuclear DNA from feces samples is reported. This method employs a stool lysis buffer and a unique matrix (InhibitEX tablet) to remove PCR inhibitory substances specific to feces samples. DNA extracted from various amounts of stool and from stool samples exposed to different environmental impacts was successfully amplified and typed using the Profiler Plus Amplification Kit and ABI PRISM 310 Genetic Analyser.  相似文献   
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Americans, particularly American intellectuals, have a deeply held tradition that ineffectual, limited government is in the public interest. The author questions this premise and suggests that when government is ineffectual, democracy itself suffers.  相似文献   
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