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排序方式: 共有109条查询结果,搜索用时 15 毫秒
31.
Netherlands International Law Review - 相似文献
32.
Karsten Nielsen Helle Smidt Mogensen Johannes Hedman Harald Niedersttter Walther Parson Niels Morling 《Forensic Science International: Genetics Supplement Series》2008,2(3):226-230
Six commercial preparations of human genomic DNA were quantified using five quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler™ Human DNA Quantification kit and RB1 rt-PCR. All methods measured higher DNA concentrations than expected based on the information by the manufacturers. UV spectrometry, SYBR-Green dye staining, slot blot and RB1 rt-PCR gave 39, 27, 11 and 12%, respectively, higher concentrations than expected based on the manufacturers’ information. The DNA preparations were quantified using the Quantifiler™ Human DNA Quantification kit in two experiments. The measured DNA concentrations with Quantifiler were 125 and 160% higher than expected based on the manufacturers’ information. When the Quantifiler™ human DNA standard (Raji cell line) was replaced by the commercial human DNA preparation G147A (Promega) to generate the DNA standard curve in the Quantifiler™ Human DNA Quantification kit, the DNA quantification results of the human DNA preparations were 31% higher than expected based on the manufacturers’ information. The results indicate a calibration problem with the Quantifiler™ human DNA standard for its use with the Quantifiler™ Human DNA Quantification kit. The possible reasons for the problem are discussed and a solution is suggested. The results emphasise the need for standard reference DNA material and standard methods for DNA quantification. 相似文献
33.
Recently, the ENFSI/EDNAP groups issued advice on the design of the next generation of STR multiplexes in order to encourage standardisation within Europe. As the result of collaborative experimentation within the EDNAP group, we demonstrated that the low molecular weight STRs had substantial benefits to detect degraded samples. We subsequently recommended adoption of three new mini-STR loci to improve the success rate of degraded DNA markers, concurrent with the reduction in size of the existing STR markers in current use. This also improves the discriminating power of the system which is important to improve the power of national DNA databases. Subsequent discussions have occurred with manufacturers and members of the ENFSI/EDNAP groups. Because significant time and investment is required to develop new multiplexes of 13+ STR loci, manufacturers indicated that it would be preferable to adopt a staged approach. Two differing, but parallel strategies have now emerged. The first strategy employs a 13 STR loci multiplex incorporating three mini-STRs into the current multiplex test. The second strategy employs a multiplex of six high molecular weight STRs (in current use), modified to provide smaller amplicons combined with an additional two loci of high discriminating power. Eventually, the two strategies will converge to provide a single multiplex of 15 STR loci. The process will be guided by the ENFSI/EDNAP groups. 相似文献
34.
Sanchez JJ Børsting C Hallenberg C Buchard A Hernandez A Morling N 《Forensic science international》2003,137(1):74-84
We have developed a robust single nucleotide polymorphism (SNPs) typing assay with co-amplification of 25 DNA-fragments and the detection of 35 human Y chromosome SNPs. The sizes of the PCR products ranged from 79 to 186 base pairs. PCR primers were designed to have a theoretical Tm of 60 +/- 5 degrees C at a salt concentration of 180 mM. The sizes of the primers ranged from 19 to 34 nucleotides. The concentration of amplification primers was adjusted to obtain balanced amounts of PCR products in 8mM MgCl2. For routine purposes, 1 ng of genomic DNA was amplified and the lower limit was approximately 100 pg DNA. The minisequencing reactions were performed simultaneously for all 35 SNPs with fluorescently labelled dideoxynucleotides. The size of the minisequencing primers ranged from 19 to 106 nucleotides. The minisequencing reactions were analysed by capillary electrophoresis and multicolour fluorescence detection. Female DNA did not influence the results of Y chromosome SNP typing when added in concentrations more than 300 times the concentrations of male DNA. The frequencies of the 35 SNPs were determined in 194 male Danes. The gene diversity of the SNPs ranged from 0.01 to 0.5. 相似文献
35.
Recent research has shown an increasing interest in the historical evolution of legislative institutions. The development of the UK Parliament has received particularly extensive attention. In this article, we contribute to this literature in three important ways. First, we introduce a complete, machine-readable data set of all the Standing Orders of the UK House of Commons between 1811 and 2015. Second, we demonstrate how this data set can be used to construct innovative measures of procedural change. Third, we illustrate a potential empirical application of the data set, offering an exploratory test of several expectations drawn from recent theories of formal rule change in parliamentary democracies. We conclude that the new data set has the potential to substantially advance our understanding of legislative reforms in the United Kingdom and beyond. 相似文献
36.
Although studies of electoral participation in established democracies are abundant, little attention has been devoted to Latin American democracies and few studies combine individual-level and contextual-level variables. We focus on electoral participation in 32 districts of a Latin American democracy, the Dominican Republic. Our research question is: how to explain the different impact of district magnitude in Latin America? Most importantly, we find that it has a negative effect on electoral participation whereas theories based on established democracies would predict the opposite. We argue that the negative effect is caused by the stronger influence of clientelism in smaller districts, which surfaces at less salient elections. This argument accounts for previously unexplained findings in studies of Latin America. 相似文献
37.
David W. Gjertson Charles H. Brenner Max P. Baur Angel Carracedo Francois Guidet Juan A. Luque Rüdiger Lessig Wolfgang R. Mayr Vince L. Pascali Mechthild Prinz Peter M. Schneider Niels Morling 《Forensic Science International: Genetics Supplement Series》2007,1(3-4):223-231
The Paternity Testing Commission (PTC) of the International Society for Forensic Genetics has taken up the task of establishing the biostatistical recommendations in accordance with the ISO 17025 standards and a previous set of ISFG recommendations specific to the genetic investigations in paternity cases. In the initial set, the PTC recommended that biostatistical evaluations of paternity are based on a likelihood ratio principle – yielding the paternity index, PI. Here, we have made five supplementary biostatistical recommendations. The first recommendation clarifies and defines basic concepts of genetic hypotheses and calculation concerns needed to produce valid PIs. The second and third recommendations address issues associated with population genetics (allele probabilities, Y-chromosome markers, mtDNA, and population substructuring) and special circumstances (deficiency/reconstruction and immigration cases), respectively. The fourth recommendation considers strategies regarding genetic evidence against paternity. The fifth recommendation covers necessary documentation, reporting details and assumptions underlying calculations. The PTC strongly suggests that these recommendations should be adopted by all laboratories involved in paternity testing as the basis for their biostatistical analysis. 相似文献
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40.
Competition policy often asks whether a “fair share” of the benefits from cost savings obtained through mergers or agreements
is passed on to the consumers. We assess the factorsthat determine cost pass-on in some partial-equilibrium oligopoly models,
and show that, although the strength of the pass-on varies from one situation to another, there are some rules of thumb that
give a first approximation of the pass-on rate. We also show that, contrary to common belief and to what is written about
the subject in the European Commission's guidelines on the application of Article 81(3), in most circumstances cost pass-on
does not depend on the price elasticity of demand nor on the market share of the cost saver, and that with competition the
pass-on of firm-specific cost savings is weaker than without.
JEL Classification: C72 (non-cooperative games), D43 (oligopoly), L40 (antitrust policy) 相似文献