Identification of N,N-dimethylamphetamine formed by methylation of methamphetamine in formalin-fixed liver tissue by multistage mass spectrometry

Methamphetamine is methylated in the presence of unbuffered formalin solutions within hours at room temperature. The product, N,N-dimethylamphetamine, is also found in human liver exposed to methamphetamine followed by incubation with formalin. In the present study, a direct mass spectrometric method was developed to identify N,N-dimethylamphetamine in human liver before and after treatment with formalin. Human liver samples were obtained from four deaths that were investigated by the West Virginia Office of Chief Medical Examiner. Full toxicological analysis was conducted on samples from the decedents and methamphetamine was among the positive findings in each case. The method used to expose liver tissue to formaldehyde involved treating a small piece of liver from each case with formalin solution (20% v/v) for 24 h at room temperature. The formalin treated tissues were homogenized and the resulting suspension was sonicated for 5 min, and then centrifuged. Supernatant aliquots were directly analyzed by electrospray ionization (ESI) mass spectrometry without chromatographic isolation. Positive ion multistage mass spectra recorded in MS, MS/MS and MS/MS/MS (MS3) modes were used to confirm the presence of N,N-dimethylamphetamine and methamphetamine in the mixture. Liver tissue not treated with formalin did not contain a detectable level of N,N-dimethylamphetamine. Decreases in methamphetamine concentrations in liver tissue resulting from treatment with formalin were measured using deuterium-labeled methamphetamine as internal standard. The method can be completed in less than 2 h on thawed tissue. The results suggest that the process of fixing tissues with formalin may lead to false negative findings for methamphetamine.     

Colonia Housing Conditions in Model Subdivisions: A Déjà Vu for Policy Makers

The informal self-help settlements in Texas known as colonias have received considerable attention as a public policy problem at both the state and federal levels. These settlements proliferated throughout the border region since the late 1970s and research has highlighted the extreme poverty, austere levels of infrastructure, exploitative land sale practices, and poor housing conditions that characterized these settlements. However, both scholars and policymakers have overlooked the continued spread of self-help settlements known as “model subdivisions,” which barring the presence of basic water, wastewater, and electricity services, are nearly identical to colonias. We present the results of household surveys conducted with residents in 24 model subdivisions in Hidalgo County, Texas, in June 2014. The results suggest that, unbeknown to legislators, many of the problems that characterized colonias are now being reproduced in hundreds of model subdivisions that have formed since the 1990s, and which now require concerted attention and intervention by policy makers.     

The Counterrevolutionary Campus: Herbert Marcuse and the Suppression of Student Protest Movements

Abstract

With the recent surge of college protests against various forms of economic, political, social, and racial injustice, there have been persistent and pernicious reactions from other students, administrators and public figures that function to undermine the emancipatory impulses animating these demonstrations. The reactions are often justified under the banners of tolerance, chastising students to listen instead of protest. This article, focusing on Marcuse’s concepts of repressive toleration and counterrevolution, evaluates the reactionary responses to these events, as well as the critical potential of this fledgling student sensibility, a burgeoning refusal represented by protest events at American universities. We maintain that many of the calls for tolerance are actually demands for silence and belong to a wider counterrevolutionary phase of late capitalism observed by Marcuse. Bedrock liberties are dialectically inverted whereby speech and toleration are repressively deployed against demands for justice. This article concludes by arguing that it is crucial to the success of this resurgent sensibility for justice—and progress toward a radical socialist movement that coincides with the emancipatory vision of Herbert Marcuse—that the counterrevolutionary character of the responses are demystified.     

Evaluation of Effectiveness of Health Services Training Given with Different Methods in Combating of Intimate Partner Violence against Women: A Pilot Study

The study was conducted to evaluate the effectiveness of health services training provided with different methods in combating of intimate partner violence (IPV) against women. The intervention group of the study consisted of 132 healthcare workers (HCW) and 249 HCWs in the control group. According to the result of study, training given to the intervention group was more effective in increasing the knowledge level, compared to the control group. Furthermore, comparison of the HCWs in the intervention group with the control group revealed that the attitudes towards emotional, psychological and sexual violence and justifying myths changed positively. In this scope, it can be said that intervention training was effective in terms of improving knowledge and attitude on IPV. However, it is determined that the training given to both groups was inefficient in terms of turning into behaviour.