Application of DNA Forensic Techniques for Identifying Poached Guanacos (Lama guanicoe) in Chilean Patagonia*

Abstract: Guanaco (Lama guanicoe) is a protected and widely distributed ungulate in South America. A poacher, after killing guanacos in Valle Chacabuco, Chilean Patagonia, transported and stored the meat. Samples were retrieved by local police but the suspect argued that the meat was from a horse. Mitochondrial cytochrome b gene (774 pb), 15 loci microsatellites, and SRY gene were used to identify the species, number of animals and their population origin, and the sex of the animals, respectively. Analysis revealed that the samples came from a female (absence of SRY gene) Patagonian guanaco (assignment probability between 0.0075 and 0.0282), and clearly distinguishing it from sympatric ungulates (E‐value = 0). Based on the evidence obtained in the field in addition to forensic data, the suspect was convicted of poaching and illegally carrying fire arms. This is the first report of molecular tools being used in forensic investigations of Chilean wildlife indicating its promising future application in guanaco management and conservation.     

Spanish Translations of Miranda Warnings and the Totality of the Circumstances

Spanish-translated Miranda warnings are administered annually to thousands of Hispanic custodial suspects. In examining 121 Spanish translations and their English counterparts from 33 states, the lengths of Miranda warnings were generally comparable but marked differences were observed in the reading levels for individual Miranda components. The adequacy of Miranda translations varies markedly from minor variations to substantive errors. The most serious problems involved the entire omission of Miranda components; several omissions were observed in the Spanish translations for even the basic rights to silence and counsel. More commonly, Miranda discrepancies involved dissimilar content with a substantial trend toward more information in English than Spanish versions. Findings related to the Miranda translations, different word lengths, and varied reading levels are discussed using the totality of circumstances as its framework.

Punishing Cruelly: Punishment, Cruelty, and Mercy

What is cruelty? How and why does it matter? What do the legal rejection of cruelty and the requirements of mercy entail? This essay asks these questions of Lucius Seneca, who first articulated an agent-based conception of cruelty in the context of punishment. The hypothesis is submitted that the answers to these questions offered in Seneca’s De clementia constitute one of the turning points in the evolution of practical reason in law. I conclude, however, by arguing that even the mainstream punitive practices of contemporary western societies fail to meet the modest imperatives of the rejection of cruelty and the unconditionality of mercy propounded by Seneca.     

Arts marketing framework: The arts organisation as a hub for participation

The proliferation of artistic content, increased mobility of people, ethnic diversification, and increased scarcity of time outside of work against a backdrop of austerity requires an integrated approach to marketing within the arts. This paper proposes a framework for the marketing of arts based on participation, cocreation, and social networks. Additionally, the framework considers the role of self-construal in the participation of arts.     

Analyzing Brazilian vehicle documents for authenticity by easy ambient sonic-spray ionization mass spectrometry

Using desorption/ionization techniques such as easy ambient sonic-spray ionization mass spectrometry (EASI-MS), it is possible to analyze documents of Brazilian vehicles for authenticity, providing a chemical profile directly from the surface of each document. A method for the detection of counterfeit documents is described, and the falsification procedure is elucidated. Forty authentic and counterfeit documents were analyzed by both positive and negative ion modes, EASI(±)-MS. EASI(+)-MS results identified the presence of (bis(2-ethylhexyl)phthalate plasticizer and of dihexadecyldimethylammonium biocide in both types of documents. For EASI(-)-MS results, the 4-octyloxybenzoic acid additive ([M + H](+): m/z 249) is present only in counterfeit documents. It was also found that counterfeit vehicle documents are produced via Laserjet printers. Desorption/ionization techniques, such as EASI-MS, offer therefore, an intelligent way to characterize the counterfeiting method.     

Genetic data on 12 STRs (F13A01, F13B,FESFPS, LPL,CSF1PO,TPOX, TH01, vWA,D16S539, D7S820, D13S317, D5S818) from four ethnic groups of São Paulo,Brazil

Allelic frequencies for 12 short tandem repeats (STRs) (F13A01, F13B, FESFPS, LPL, CSF1PO, TPOX, TH01, vWA, D16S539, D7S820, D13S317 and D5S818) were estimated, also as forensic parameters, from a sample of 916 unrelated Brazilian subjects classified into four ethnic groups: European-derived, African-derived, Brazilian Mulattos and Asian-derived.