SNPs in paternity investigation: The simple future

Based on the 52 SNP-plex developed by the SNPforID Consortium, we designed two 10-plex to study single nucleotide polymorphisms (SNPs) for human identification and to establish its usefulness in paternity casework. This 20 autosomal SNP set was studied in 56 paternity investigation cases from South Portuguese resident population, also analyzed with 17 Short Tandem Repeats (STRs). Results obtained with both methodologies were consistent with each other, except for one case where the alleged father could not be excluded by SNPs. No mutation was found in the SNP loci, whereas a mismatch in STRs was detected. The use of SNPs as a complement to the analysis of autosomal STRs in paternity casework can result in paternity index and paternity probability values equivalent or higher than those obtained with more STR loci, but with lower costs. This study shows that instead of using additional STR loci, the analysis of 20 autosomal SNPs, as a complement technique to standard methodologies, is an appealing alternative in paternity investigation cases.     

MtSNP typing before mtDNA sequencing: Why do it?

Analysis of control mitochondrial DNA (mtDNA) hypervariable regions is sometimes the only available method to study hair evidence in forensic casework although being a laborious technique. Nowadays there is a huge interest in new genetic markers such as single nucleotide polymorphisms (SNPs) to type degraded forensic samples. For that purpose, a 10-Plex mitochondrial SNP for haplogroup typing, chosen from several SNP studies and useful to study the most common populations in our laboratory was applied in forensic casework. Hair shafts from three forensic cases with different ethnic backgrounds were studied with mtDNA sequencing and compared with mitochondrial SNPs (mtSNPs) study. Coding mtSNP typing prior to sequencing can allow for a rapid screening in forensic casework, which is emphasized in the first two cases. Moreover, in cases in which mtDNA sequencing fails, mtSNPs can still be detected. This 10 SNP loci multiplex provides a less expensive and simpler method for mitochondrial typing compared to control region mtDNA sequencing, especially when used as a fast screening method.     

Extraction and isolation of cannabinoids from marijuana seizures and characterization by 1H NMR allied to chemometric tools

Marijuana, a drug derived from the Cannabis sativa L. plant, is the world's most consumed illicit drug. In this paper, a total of 156 marijuana samples seized in the state of Espírito Santo (ES), Brazil were studied and analysed by proton nuclear magnetic resonance (¹H NMR) spectroscopy to identify the major cannabinoids present. A crude extract of all samples was purified using high performance liquid chromatography so that these compounds could serve as reference substances. Nine fractions were obtained and analysed by ¹H NMR and gas chromatography–mass spectrometry (GC–MS), with five presented cannabinoids. ?⁹-THC (Δ⁹-trans-tetrahydrocannabinol), ?⁹-THCA (?⁹-tetrahydrocannabinolic acid), ?⁸-THC (?⁸-tetrahydrocannabinol), 11-hydroxycannabinol, CBV (cannabivarin), and CBN (cannabinol) were found, and their chemical structures were confirmed by GC–MS. The latter compound was obtained with high purity (≈100%), while the others were obtained as less complex mixtures with purity higher than 75% (except for Δ⁸-THC). Principal component analysis (PCA) was used on the ¹H NMR spectra of the 156 samples, and it was found that the samples were grouped according to the months, differentiating into two groups (from July 2014 to January 2015 and from February 2015 to July 2015), where non-grouping was observed from four macro-regions of the ES state (North, Central, Metropolitan, and South). The chemical profile of the seized samples was correlated to the ¹H NMR spectrum of an isolated CBN sub-fraction, in which the group formed by samples seized in the year 2015 presented lower CBN content in the chemical composition. From the PCA score plot, two groups of samples were confirmed using the partial least squares discriminant analysis and orthogonal projections to latent structures classification methods.     

A Feasibility Pilot Intervention Program to Teach Parenting Skills to Mothers of Poly-Victimized Children

The Parceria (Partnership) Project is a Brazilian intervention program designed for mothers with an intimate partner violence (IPV) history. Its short term goal is to teach parenting skills to abused women, and in the long term, to prevent behavioral problems in their children. The objective of this pilot study was to evaluate the feasibility of the Parceria Project with mothers of children who had experienced multiple forms of maltreatment (poly-victimization), as it was expected that most of the mothers would also have a history of IPV. Seventeen Brazilian mothers took part in this intervention. They completed several types of evaluation. The intervention program using a cognitive-behavioral model took place in each family’s home. All mothers completed the intervention and evaluated the project positively. The study showed that it is feasible to conduct interventions with families who face severe psychosocial risk such as family violence.     

Citizenship and justice: public prosecutors in social contexts in Portugal

All over the world judicial systems are under tremendous pressure as the instruments used by citizens to access their full rights. The erosion of other state powers has transferred expectations of social intervention or, at least, protection for the rights of the weak and vulnerable, to the sphere of justice. Hence, in some countries the social role of judges or public prosecutors has become more important and their work is publicly scrutinised to ensure that their duties are performed correctly and fairly. In addition to criminal law, social areas of justice (concerning workers and children) have become more central to judicial systems, conferring a new public responsibility on these professionals.

In several countries, including Portugal, public prosecutors are unusual within the legal profession given that they have equal status in both social and criminal areas of law. In certain systems, public prosecutors may act as a party, defending the rights of powerless citizens and leading them through the judicial process. Such powers offer great potential for fairness and justice but at the same time can lead to dangerous professional controversies. Through an analysis of the Portuguese model, one of the more advanced of its kind (in terms of intervention), some of the main features will be described and identified.

Public prosecutors in Portugal have, for many years, been in charge of a set of very varied responsibilities within the context of the Family and Juvenile and Labour Courts which far exceed what is publicly acknowledged, particularly in criminal matters. However, their functions are not limited to those of the ‘public prosecutor’ or ‘coordinator of the investigation’ typically associated with responsibilities in criminal matters.

Within the context of these two major and socially sensitive areas, public prosecutors act as intermediaries between the different parties and entities involved in litigation, a fact which, in professional terms, endows them with features which are atypical of magistrates and places them in close contact with citizens. Thus, taking a case study based on the Coimbra Family and Juvenile and Labour Courts as its starting point, this paper aims to map out these formal and informal functions, which create a level of importance that is probably much higher than would have been expected, particularly given the lack of truly credible and effective alternatives that enable citizens to access law and justice.     

La descentralización de los procesos de selección de candidatos en los partidos y su impacto en la nominación de mujeres en los Congresos Estatales de México

The analysis of state democratization includes recent studies of the methods by which political parties are selected, and their possible effect on the political participation of women. Considering the renewal of Mexican federalism, which took place since the dismantling of the presidential regime at the end of the 1990s, this article brings together issues of decentralization and party nomination processes, as well as “selectorate agencies” and the formal institutions that limit them in order to question the effect of the combined presence of these factors on women’s political participation patterns. The article analyzes local election data from twelve states in Mexico, focusing on the period 1998 to 2012. The evidence reveals that in the case of Mexico, decentralization has had no effect on nominations made by a relative majority. Furthermore, it suggests that centralization in decision-making is an important factor in achieving the nomination of more women based on the principle of proportional representation.     

Class‐Biased Electoral Participation: The Youth Vote in Chile

There is no other country where the division between the old and young electorate is as striking as in Chile. For older voters, turnout exceeded, on average, 90 percent in 2009; for those aged less than 30, it fell below 30 percent. Using individual survey data from 2006, 2008, and 2010, this article studies the current socioeconomic composition of the Chilean young electorate. First, it shows that the young electorate is class‐biased. Income is highly correlated with both registration and turnout even after controlling for education. Second, it presents evidence that class bias for the whole electorate has been increasing over time, due to generational replacement. The results are not promising for Chile's democracy in the years to come, since equal participation is worsening over time.     

Personal identification and missing persons initiatives in Santa Catarina state,Brazil: forensic perspectives from 2019 to 2021

Santa Catarina is a small, developed, and relatively safe state in South Brazil. Despite having positive social economic indicators, it still faces multiple challenges regarding forensic practices for personal identification. The objective of this paper is to discuss the recent advances and current challenges in the region, from the perspectives of anthropological and dental postmortem human identification, missing persons, and disaster victim identification (DVI) from 2019 to 2021. The recent creation of a Forensic Anthropology Sector (SAF) in the state’s official forensic institution (Polícia Científica—PCI) has significantly improved identification of unidentified remains and optimised resources available for DNA analysis. However, SAF is still quite understaffed, which negatively affects the recovery of skeletal material, its preparation, and the time needed for filing reports. Santa Catarina has passed legislation for missing persons in 2015, 4 years prior to the sanction of federal laws implementing the national policy for the disappeared. Nonetheless, a lack of integration between stakeholders remains a problem that PCI has tried to circumvent with the Conecta Programme, a multidisciplinary and integrated initiative between families of the missing persons, police agencies, and the Public Ministry. The programme aims to collect not only reference DNA samples, but also relevant anthropological and dental data. It also offers facial progression services in cases of disappearances that occurred many years ago. Despite a history of disasters in the state, PCI still needs to implement international DVI standards at an institutional level. Recent training on Phase 1 DVI procedures, integrated with other responding institutions, indicates better preparation for future disasters. There are many challenges ahead for Santa Catarina’s forensic institution and professionals that have yet to be addressed, but the overall situation on routine personal identification, missing persons initiatives, and DVI has improved over the last 2 years.     

The Due Diligence Standard and the Prevention of Racism and Discrimination

Netherlands International Law Review - The due diligence standard has played a significant role in preventing human rights violations, including racial discrimination. Yet, it is significantly...     

The Impact of Procedural Information Costs on Voting: Evidence from a Natural Experiment in Chile

This paper uses a natural experiment to identify the effect of a procedural information cost on the electoral registration of young first-time voters. We exploit the fact that information about when the minimum age eligibility requirement is due, either at registration or election-day, is only meaningful for those turning 18 after registration closing day. Using a national dataset on Chilean registration over four elections, we provide evidence of a sharp discontinuity in the registration rate of those youngsters turning 18 at closing date. The effect is both sizable and robust, persists over time, and is similar across income groups.