A Process Evaluation of a Kentucky Court Improvement Initiative

This article describes a court improvement initiative designed to promote uniformity and improved court practice with the ultimate goal of the improvement of outcomes for children and families. The article focuses on the results of interviews and focus groups conducted as part of the evaluation of this initiative. Twelve jurisdictions were purposively selected to exhibit a range of family court and non–family court jurisdictions in rural and middle‐sized locations. The discussion of the qualitative results focuses on changes in court practice and the impact of the initiative on case time.     

Quantitative PCR overestimation of DNA in samples contaminated with tin

Metals can pose challenges while conducting forensic DNA analysis. The presence of metal ions in evidence-related DNA extracts can degrade DNA or inhibit PCR as applied to DNA quantification (real-time PCR or qPCR) and/or STR amplification, leading to low success in STR profiling. Different metal ions were spiked into 0.2 and 0.5 ng of human genomic DNA in an “inhibition study” and the impact was evaluated by qPCR using the Quantifiler™ Trio DNA Quantification Kit (Thermo Fisher Scientific) and an in-house SYBR Green assay. This study reports on a contradictory finding specific to tin (Sn) ions, which caused at least a 38,000-fold overestimation of DNA concentration when utilizing Quantifiler Trio. This was explained by the raw and multicomponent spectral plots, which indicated that Sn suppresses the Quantifiler Trio passive reference dye (Mustang Purple™, MP) at ion concentrations above 0.1 mM. This effect was not observed when DNA was quantified using SYBR Green with ROX™ as the passive reference, nor when DNA was extracted and purified prior to Quantifiler Trio. The results show that metal contaminants can interfere with qPCR-based DNA quantification in unexpected ways and may be assay dependent. The results also highlight the importance of qPCR as a quality check to determine steps for sample cleanup prior to STR amplification that may be similarly impacted by metal ions. Forensic workflows should recognize the risk of inaccurate DNA quantification of samples that are collected from substrates containing tin.     

A Review of: “Michael Kenney. From Pablo to Osama: Trafficking and Terrorist Networks,Government Bureaucracies,and Competitive Adaptation”

This article seeks to provide a comprehensive analysis of the early demise of Rode Jeugd's initiatives towards a terrorist campaign in the Netherlands. It is concluded that three contextual factors played a crucial role in the process: the openness of the political culture, the setbacks experienced by similar organizations elsewhere (RAF in particular), and the lack of overt repression, combined with effective covert operations carried out by the security forces against the Rode Jeugd. Overall, the case of Rode Jeugd is a prime example of a demonstration of state power not always being the most efficient or even suitable method to combat terrorism.     

Andreas Wenger and Reto Wollenmann,eds. Bioterrorism: Confronting a Complex Threat

This article examines the transformation of the Chechen conflict from a predominately nationalist to jihadist struggle, and compares the similar changes that took place in the Kashmiri insurgency. Using global jihadist strategy and ideology, and the accompanying influence of Al Qaida, both conflicts are shown to have taken on a new ideology and to have expanded beyond previous areas of operation. In both instances, the political leadership wrapped themselves in the mantle of political Islam (Islamism) as ensuing violence led to rapid socioeconomic transformation and social breakdown, thus allowing foreign jihadists to exert power and take up/divert the cause. In the past few years, two main groups originating in Chechnya and Kashmir have taken on Western targets and become more indoctrinated in Al Qaida's global jihadist ideology: the Caucasus Emirate (CE) and Lashkar-e-Taiba (LeT). The opportunist franchising strategy of Al Qaida could come to play a role in the future of both groups, especially if the CE is able to coalesce into a more unified front. More importantly, the global jihadist attributes of the CE must begin to garner the same attention in the Western world as that of LeT.