A novel histological technique for distinguishing between epithelial cells in forensic casework

There are a number of forensic cases in which the identification of the epithelial cell type from which DNA originated would provide important probative evidence. This study aimed to develop a technique using histological staining of fixed cells to distinguish between skin, buccal and vaginal epithelium. First, 11 different stains were screened on formalin-fixed, wax-embedded cells from five women. Samples were analysed qualitatively by examining staining patterns (colour) and morphology (absence or presence of nuclei). Three of the staining methods – Dane's, Csaba's and Ayoub-Shklar – were successful in distinguishing skin epithelial cells from buccal and vaginal. Second, cells were smeared directly onto slides, fixed with one of five fixatives and stained with one of the three stains mentioned above. Methanol fixation, coupled with the Dane's staining method, specific to keratin, was the only technique that distinguished between all three cell types. Skin cells stained magenta, red and orange and lacked nuclei; buccal cells stained predominantly orange–pink with red nuclei; while vaginal cells stained bright orange with orange nuclei and a blue extracellular hue. This staining pattern in vaginal cells was consistent in samples collected from 50 women aged between 18 and 67. Identification of cell type from unlabelled micrographs by 10 trained observers showed a mean success rate of 95%. The results of this study demonstrate that histological staining may provide forensic scientists with a technique for distinguishing between skin, buccal and vaginal epithelial cells and thus would enable more conclusive analyses when investigating sexual assault cases.     

Managing incidental findings in human subjects research: analysis and recommendations.

No consensus yet exists on how to handle incidental findings (IFs) in human subjects research. Yet empirical studies document IFs in a wide range of research studies, where IFs are findings beyond the aims of the study that are of potential health or reproductive importance to the individual research participant. This paper reports recommendations of a two-year project group funded by NIH to study how to manage IFs in genetic and genomic research, as well as imaging research. We conclude that researchers have an obligation to address the possibility of discovering IFs in their protocol and communications with the IRB, and in their consent forms and communications with research participants. Researchers should establish a pathway for handling IFs and communicate that to the IRB and research participants. We recommend a pathway and categorize IFs into those that must be disclosed to research participants, those that may be disclosed, and those that should not be disclosed.     

16 STR data of a Greek population

Allele frequencies for 16 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA, and SE33) were calculated for a sample of 300 unrelated individuals from Greece. No deviations from Hardy–Weinberg equilibrium were observed for all loci. The combined power of discrimination (PD) and the combined power of exclusion (PE) for the 16 tested STR loci were 0.999999999 and 0.999999816, respectively. Population comparisons were carried out and low genetic distances were found between our data and those previously published for other neighbouring European populations.     

Posttraumatic Stress Disorder: Current Concepts and Controversies

The diagnosis of posttraumatic stress disorder (PTSD) sometimes is raised in compensation claims, in tort settings, and in other medical–legal settings. Accordingly, health-care and legal professionals working in these areas need to be familiar with the current findings and controversies concerning the disorder. The purpose of this article is twofold. First, we review the most important findings concerning the clinical features, etiology, and treatment of PTSD. Second, we examine six major controversies concerning the disorder that are relevant to psychologists and other medical–legal practitioners: (a) the issue of what qualifies as a traumatic stressor, (b) the question of whether traumatic stress causes brain damage, (c) the validity of the concept of delayed-onset PTSD, (d) the recovered memory controversy, (e) the question of whether PTSD can arise when the person has no memory of the trauma (e.g., due to concussion), and (f) issues concerning PTSD malingering. Throughout this article we offer recommendations for psychological and other medical–legal practice in relation to the evaluation of PTSD claims.     

Interrogation of short tandem repeats using fluorescent probes and melting curve analysis: A step towards rapid DNA identity screening

Current forensic DNA profiling methods rely on the analysis of samples at specialised laboratories with an average turnaround time of several days. The ability to rapidly determine a partial profile of short tandem repeats at the point-of-arrest would be of great benefit to police forces around the world, for example enabling a suspect to be rapidly included or excluded from an investigation. We have developed a homogeneous PCR method for the interrogation of STR loci utilising fluorescent oligonucleotide probes and melting curve analysis. Alleles of the D18S51, TH01 and D8S1179 loci were differentiated and identified on the basis of target length and probe melting temperature. Assay performance was evaluated by comparing melting peak data with the AmpFlSTR^® SGM Plus^® system. The method is compatible with direct analysis of unpurified buccal swab samples, enabling a partial STR profile to be generated within 1 h.     

Britain's ‘betrayal’ of Hong Kong: A second look

Claims of British bungling and betrayal were repeatedly raised during the prolonged run‐up to the 1997 Hong Kong handover. This paper seeks to evaluate such claims. It examines five controversial episodes of reputed British mishandling of the Hong Kong transition: (1) Governor Murray MacLehose's reputed double faux pas of March 1979, when the governor first putatively erred by prematurely (and unnecessarily) raising the ‘1997 question’ in a routine meeting with Deng Xiaoping and then compounded his error by misrepresenting Deng's less‐than‐reassuring response; (2) Prime Minister Margaret Thatcher's 1982 miscalculation in requesting a 50‐year extension of the New Territories lease when it was already abundantly clear that Chinese leaders regarded the original lease as ‘unequal'—and therefore non‐binding; (3) Britain's 1987 decision to unilaterally postpone Hong Kong's first direct legislative elections, a decision defended on the disingenuous grounds that government surveys had revealed a majority of the Hong Kong public to be opposed to rapid democratization; (4) Britain's ostensible failure, in the aftermath of the 1989 Tiananmen debacle, to secure the inclusion of adequate democratic safeguards in the final draft of the Hong Kong Basic Law, completed early in 1990; and (5) Governor Chris Patten's ill‐starred democratic reform initiatives of 1992–95, which sharply reversed Britain's previous ‘convergence’ policy and ultimately led to the derailing of Hong Kong's vaunted ‘through train’. Examining available evidence on these five episodes (and one or two other, related cases), this paper argues that despite occasional, palpable policy miscalculations and a notable lack of official candor, Britain did not do serious harm to the vital interests of Hong Kong. On the contrary, the paper argues, given the substantial advantage in resources and leverage enjoyed by the Chinese side throughout the transition period, a more favorable outcome could hardly have been achieved. The one residual allegation of British betrayal that cannot readily be countered, however, is the charge that racism within Britain's ruling Conservative Party—manifested in Parliamentary legislation severely restricting the flow of immigrants from British colonial territories—played a significant role in the British Government's choice of tactics in dealing with the ‘1997 question’.     

Political Data in 1997

European Journal of Political Research -