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331.
Darrell O. Ricke Ph.D. Philip Fremont‐Smith M.S. James Watkins B.S. Sara Stankiewicz M.S. Tara Boettcher B.S. Eric Schwoebel Ph.D. 《Journal of forensic sciences》2019,64(5):1468-1474
High‐throughput sequencing (HTS) of large panels of single nucleotide polymorphisms (SNPs) provides an alternative or complimentary approach to short tandem repeats (STRs) panels for the analysis of complex DNA mixture forensic samples. For STRs, methods to estimate individual contribution concentrations compare capillary electrophoresis peak heights, peak areas, or HTS allele read counts within a mixture. This article introduces three approaches (mean, median, and slope methods) for estimating individual DNA contributions to forensic mixtures for HTS/massively parallel sequencing (MPS) SNP panels. For SNPs, the major:minor allele ratios or counts, unique to each contributor, were compared to estimate contributor proportion within the mixture using the mean, median, and slope intercept for these alleles. The estimates for these three methods were typically within 5% of planned experimental contributions for defined mixtures. 相似文献
332.
Noelia I. Zanetti DB Andrea Costantino DChem Natalia Lazzarini BSChem Adriana A. Ferrero DB Néstor D. Centeno DB 《Journal of forensic sciences》2021,66(1):245-254
Fluoxetine is a selective serotonin reuptake inhibitor, commonly used for the treatment of a variety of psychopathological conditions. As such, fluoxetine may be expected to appear in clinical and forensic cases. Dermestes maculatus De Geer (Coleoptera: Dermestidae) has been recognized as a relevant component of the insect fauna associated with decomposing human and animal remains. Experiments were conducted to study the effect of fluoxetine on developing D. maculatus using two‐drug administration models: a non‐living animal model (pork muscle) and a living one (Sus scrofa L. pigs). We assessed the duration of immature stages and total life cycle, as well as morphological parameters (body length, cephalic width, and weight). The effect of fluoxetine was studied at an overdose concentration: In the non‐living animal model the drug was mixed with macerated pork muscle (2000 mg/kg) and in the living animal model, pigs were given the drug orally (833 mg/kg). A control was used for each model. Daily observations were performed from the beginning to the end of the experiments. GC‐MS was used for drug detection and quantification. There were no statistically significant differences in the duration of immature stages, life cycle, larval mortality, morphological parameters, or sex ratio, between treatment and control, regardless of the drug administration model. Given that fluoxetine had no detectable effect on the development of D. maculatus, detection of this drug in forensic situations would not compromise the accuracy of PMI estimations. 相似文献
333.
Jeffrey D. Pugh 《拉美政治与社会》2017,59(3):98-121
This article investigates political opportunities and constraints associated with incorporating the concept of universal citizenship into migration debates. Analyzing the speeches of Ecuador's president Rafael Correa over eight years, the article argues that Correa strategically crafted a narrative of universal citizenship to undergird politically beneficial policies. Political constraints from constituents and rivals, and the populist nature of his governing style, hollowed out progressive migration policy innovations to the point that universal citizenship became a rhetorical device more than a substantive policy agenda. Through this empirical case, the article develops a more nuanced critical understanding of universal citizenship discourses as sites for negotiating the relationship between states and migrants. 相似文献
334.
335.
MALCOLM D. ROWAT 《公共行政管理与发展》1996,16(4):397-411
Public sector reform in both Latin American and Caribbean countries has become a high priority for governments in their search for a new role for the state. Common principles have included the objective to privatize, or contract out services or responsibilities where the private sector has a comparative advantage while at the same time improving government efficiency in areas that are considered core government functions at different levels of government. These include integrated government financial management, social security and social safety nets, tax administration, provision of basic services (education and health), legal/regulatory reform, and judicial enforcement. Guiding principles in all countries have been predictability, transparency and accountability. While the degree of reform has varied amongst countries, there are a number of distinguishing features of the Commonwealth Caribbean that set its public sector reform experience apart from that of Latin America. These include the legacy of a professional civil service, long-standing democratic institutions and an active civil society, the relatively small size of the countries, and the emphasis on rule of law that can help explain some of the differences in the reform paths taken. Nevertheless, government ownership and commitment to public sector reform has been shown to be the most important element in determining results within the Latin American and Caribbean region. 相似文献
336.
Jarman Hannah K. Marques Mathew D. McLean Siân A. Slater Amy Paxton Susan J. 《Journal of youth and adolescence》2021,50(12):2279-2293
Journal of Youth and Adolescence - Adolescents are spending considerable time on social media, yet it is unclear whether motivations for social media use drive different forms of social media... 相似文献
337.
Paul D. Williams 《澳大利亚政治与历史杂志》2021,67(1):150-168
This article explores the role of Queensland voters in the 2019 Australian federal election. The article identifies five key elements of a Queensland political culture before offering evidence that the Liberal-National Coalition's exceedingly strong performance in Queensland in 2019 was not a single aberration, but one of a long and continuing pattern of electoral nonconformity. The article also argues Queensland's complex regional diversity necessitates analysis across not one or two constituencies but via six geographically, economically and demographically diverse regions. Third, the article argues the unexpectedly large swing against the Australian Labor Party in Queensland in 2019 was fuelled, overwhelmingly, by five factors, each consistent with the five core elements of a Queensland political culture. 相似文献
338.
This article employs the concepts of security culture and normlocalization to explore some of the cultural dimensions of theAfrican Union's (AU) security policies. After providing an overviewof constructivist accounts of norm socialization in internationalrelations, I use these insights to analyse the origins and developmentof the AU's security culture. The final two sections explorethe ongoing process of norm localization in relation to thetwo most recent tenets of the AU's security culture: intoleranceof unconstitutional changes of government and the responsibilityto protect principle. An awareness of the uneven and contestednature of this process helps account for the fact that althoughthese two transnational norms have been institutionalized inthe AU Charter and endorsed by the United Nations, they havebeen internalized unevenly by the AU's member states. Externaladvocates of these two norms would thus do well to help thecontinent's norm entrepreneurs build congruence between thesenorms and the AU's security culture. 相似文献
339.
The Herero were the first ethnic group to be subjected to genocidein the twentieth century. In 2001, the Herero became the firstethnic group to seek reparations for colonial policies thatfit the definition of genocide. The Herero are the latest plaintiffto use the procedures of the Alien Torts Claim Act of 1789 toseek reparations in a US federal court for war crimes committedoverseas. This article analyzes the legal arguments by Hererosagainst Germany within the context of current understandingsof international law and identifies the challenges that lieahead for this claim. The article also explores the implicationsof the Herero claim for other ethnic groups victimized by colonization. 相似文献
340.