Development and validation of a liquid chromatography-tandem mass spectrometry assay for hair analysis of atomoxetine and its metabolites: Application in clinical practice

Atomoxetine (ATX) is a potent inhibitor of the noradrenaline reuptake transporter approved since 2002 for the treatment of attention-deficit/hyperactivity disorder (ADHD) in children, adolescents, and adults as alternative treatment to methylphenidate. A procedure based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the determination of ATX and its main metabolites (4-hydroxyatomoxetine - 4 hydroxyATX - and N-desmethylatomoxetine - des-methylATX) in hair of one treated child and five treated adolescents. Since hair samples can be easily collected without the need for specials skills and exposing a patient to discomfort, hair testing of ATX and eventually of its metabolites should be useful, especially in case of pediatric patients, to check compliance in a wider time-window. After addition of duloxetine as internal standard, hair samples were overnight digested with 2ml 1M NaOH at 45°C. Then, analytes were extracted from alkaline solution with two different 2ml aliquots of tert-butyl methyl ether. Chromatographic separation was achieved at ambient temperature using a reverse-phase column and a mobile phase of 40% of water-60% 5mM ammonium acetate, 50mM formic acid, 4mM trifluoroacetic acid in acetonitrile-water (85:15, v/v). The mass spectrometer was operated in positive ion mode using multiple reaction monitoring. The method was linear over the concentration range 0.2-50ng/mg hair for the all analytes under investigation, with an intra- and inter-assay imprecision and inaccuracy always less than 20% and an analytical recovery between 33.1% and 76.1%, depending on the considered analyte. Only ATX and 4-hydroxyATX were detected in hair samples with concentrations varying from 0.2 to 2.0ng/mg hair and from 0.3 to 1.0ng/mg, respectively. Notwithstanding the absence of any dose-hair concentration relationship, hair monitoring of ATX and concomitant medications commonly administrated in ADHD children and adolescents can be crucial in verifying long-term compliance to prescribed medication in individuals displaying a non negligible tendency to refuse drugs and to lie on the adherence to therapy as a specific symptom of the disease.     

Sex,sex roles,and self-image

The purpose of this research was to compare the self-images of male and female children in order to determine whether females were at a particular disadvantage and, if so, why. A random sample of 1988 children from grades 3–12 were interviewed in Baltimore in 1968. Findings show more disturbance among White adolescent females than among White males or Black females: White girls become much more self-conscious and show greater self-image instability and somewhat lower self-esteem. Three sets of factors appear to explain part of these differences: (1) attitudes toward present and future sex role, (2) peer relationships in general and opposite sex relationships in particular, and (3) attitudes toward changing looks in adolescence.The work of the first author is currently supported by a Research Development Award from the National Institute of Mental Health, No. 5-K1-MH-41, 688-03. The work was also partly supported by USPHS Grants 1-F3-MH-41, 688-01 and MH-197541-01.The data were collected in 1968.Received her Ph.D. from Columbia University and has been interested in the social determinants of the self-image and in studies of the social psychological consequences of organ transplantation.Received her Ph.D. from the University of Maryland and is interested in social psychology in general and sex roles in particular.     

Decentralization and Corruption: Evidence from U.S. Federal Transfer Programs

While some recent evidence suggests that more decentralizationis associated with reduced corruption, no empirical work hasexamined whether different types of decentralization havedifferential effects on corruption. The theoretical literaturehas emphasized that expenditure decentralization will only beeffective if accompanied by the devolution of revenuegeneration to local governments. In this paper we examine thishypothesis empirically, by studying the mismatch betweenrevenue generation and expenditure in U.S. states. Largerfederal transfers are associated with higher rates ofconviction for abuse of public office, supporting the theorythat soft-budget constraints created by federal transfers arepotentially problematic.     

Parental contexts of adolescent self-esteem: A developmental perspective

Relationships between parental behaviors and adolescent self-esteem were analyzed in a group of 95 early adolescents from multiple settings. The study was designed to investigate hypotheses regarding associations between observed parental interactions (e.g., accepting and devaluing) and adolescent self-esteem. Parents' verbal interactions with their adolescents were assessed through application of the constraining and enabling coding system to transcribed family discussions, generated through a revealed differences procedure. Adolescent self-esteem was measured with the Coopersmith Self-Esteem Inventory. Parent interaction-self-esteem associations were examined in the pooled sample, as well as in specific sub-groups based on gender, health, and ego development (measured by the Washington University Sentence Completion Test). Boys had more numerous associations between their self-esteem and parental interactions than girls, and psychiatrically ill boys had particularly high associations. Parental interactions were found to be most strongly related to adolescent self-esteem for adolescents at the lowest levels of ego development. Our findings are consistent with the view that increasing individuation in self-esteem regulation occurs during adolescent development, such that adolescents at higher levels of ego development evaluate themselves more independently of parental feedback than do their less mature peers.This study was supported through a Research Training Grant No. MH16259 (Dr. Isberg) from the NIMH, a grant from the National Institute of Child and Human Development (NICHD Grant No. 5 R01 HD18684-02), and a Research Scientis Development Award No. 5 K-02-MH-70178 (Dr. Hauser) from the NIMH.Received M.D. from Harvard University. Currently studying adolescent development and working with the school consultation program of the Massachusetts Mental Health Center.Received M.D. from Yale University and Ph.D. from Harvard University (Psychology). Currently studying family contexts of adolescent development.Received M.D. from The University of Chicago. Currently studying psychological consequences of diabetes mellitus.Received Ed. D. from Harvard University (School of Education). Currently studying family coping processes in response to stressful events.Received Dipl. Psych. from Freie Universitat, Berlin (Clinical Psychology). Currently studying relationships between psychopathology and development among adolescent psychiatric patients.Received Ph.D. from Ohio State University (Psychology). Current interests in assessing ego development and family systems.Received Ph.D. from the University of Miami (Clinical Psychology). Research interests in family studies and adolescent development.     

The Adolescent Society Revisited: Cultures, Crowds, Climates, and Status Structures in Seven Secondary Schools

The authors used data from seven schools in a metropolitan region to explore continuity and change in adolescent cultures and status structures; they identified six major types of status structures and explained variation among them in terms of characteristics of communities and schools. A key feature of the study is comparison of school climates, using both survey data and qualitative case studies that provide an interpretive context for the survey responses; this methodology allows a contextual level of analysis that is unavailable in either single-school ethnographies or large data sets that aggregate responses from individuals situated in many schools.Roberta Garner is Professor of Sociology at DePaul University. She received a Ph.D. in sociology from the University of Chicago (1966). Her interests are in sociological theory, political sociology, and the sociology of culture.Judith Bootcheck is Associate Professor of Sociology at DePaul University and received the Ph.D. from Purdue University (1969). Her interests are in youth and the aging, families, and social problemsMichael Lorr is a Ph.D. student in Urban Studies at University of Wisconsin-Milwaukee. He received an MA in sociology from DePaul (2002) and his interests are in sociology of culture, political sociology, and sociology of educationKathryn Rauch received an MA from DePaul University with a focus on sociology of education and youth     

Effects of girls' hormonal status on depressive and aggressive symptoms over the course of one year

Associations between hormonal and physical status and girls' depressive affect, aggressive affect, and delinquent behavior were studied over the course of one year. Seventy-two White girls, aged 10–14 at initial data collection, were seen twice. Endocrinological status (estradiol, luteinizing hormone [LH], folicle stimulating hormone [FSH], testosterone, and dehydroepiandosterone sulfate [DHEAS] at Time 1, physical development (menarche, secondary sexual characteristics) and maturational timing at Times 1 and 2 were used to predict self- and maternal reports of depressive affect, and self-reports of aggressive affect and delinquent behavior at Time 2. It was posited that initial endocrinological status, as represented by hormonal categories derived by Warren and Brooks-Gunn [(1989) Mood and Behavior at Adolescence: Evidence for Hormonal Factors, Journal of Clinical Endocrinology and Metabolism, Vol. 69, pp. 77–83] and reflecting estradiol, LH, and FSH levels, would be associated with affective expression and delinquent behavior one year later. DHEAS, however, was not expected to be associated with affective expression a year later as it is thought to be more indicative of adrenal changes that are in part environmentally mediated. Initial affective expression was hypothesized to account for more of the variation than prior hormonal status. Hormonal categories predicted depressive and aggressive affect a year later, while DHEAS, physical status, and maturational timing did not. Initial hormonal categories were associated with subsequent reports of delinquent behavior, although not after physical status or prior reports of delinquency were entered into the regression. Initial reports of affective expression accounted for a far greater proportion of the variance in aggressive affect and delinquent behavior than did hormonal or physical status.Received Ph.D. from Institute of Child Development, University of Minnesota. Research interests include the interpaly among developmental processes during the transitions into and out of adolescence.Received Ph.D. from University of Pennsylvania. Research interests include Girl's psychological adaptation to pubertal change, biosocial aspects of female reproductive events, development of biological and socially at risk children and adolescents.Research interests include: Reproductive endocrinology, anorexia nervosa, neuroendocrinology, adolescence.     

Friedrich Christoph Dahlmann und die „gute“ Verfassung

The primary focus of the historian and political scientist Friedrich Christoph Dahlmann (1785–1860) was the importance of constitution. Dahlmann perceived the concept of constitution not as a written legal document but rather, following the thinking of Aristotle, as the good order of a political community. This constitutional concept was Dahlmann’s guide in dealing with three particular political challenges: the quest for Schleswig-Holstein’s unity with the German nation, his leadership in the protest of seven Göttingen professors against the constitutional coup of the new king of Hanover in 1837, and his contribution to the German Constitution of 1849. Dahlmann’s main legacy is the importance of integrating constitutional topics into today’s research and teaching of politics and polity.