间接ELISA检测鸡大肠埃希氏菌菌毛抗体方法的建立

用大肠埃希氏菌O1、O2、O3、O78和O89株制备ELISA抗原,与抗鸡Ig单抗1B7酶结合物建立了检测鸡血清抗体水平的间接ELISA方法.经交叉试验、阻断试验、重复性试验等表明该方法重复性好、特异性强、灵敏度高;确立了将鸡血清100倍稀释检测ELISA效价(ET)的回归方法y=2.453+0.131x(P＞0.05),可用于定量测定.用IH与ELISA方法的比较试验表明,ELISA法比IH法敏感性高4-100倍.     

深入推进学习型党组织建设积极创建学习型学校——学习型学校建设的案例研究

学习型学校的核心是学习,学习成熟是一所学校成熟的重要标志。北京宣武红旗业余大学坚持以学习型党组织创建来带动学习型学校的建设,以团队学习来传播学校的核心理念,党政合力,创建双赢。     

RNICE Model: Evaluating the Contribution of Replication Studies in Public Administration and Management Research

Replication studies relate to the scientific principle of replicability and serve the significant purpose of providing supporting (or contradicting) evidence regarding the existence of a phenomenon. However, replication has never been an integral part of public administration and management research. Recently, scholars have called for more replication, but academic reflections on when replication adds substantive value to public administration and management research are needed. This article presents the RNICE conceptual model, for assessing when and how a replication study contributes knowledge about a social phenomenon and advances knowledge in the public administration and management literatures. The RNICE model provides a vehicle for researchers who seek to evaluate or demonstrate the value of a replication study systematically. The practical application of the model is illustrated using two published replication studies.     

新疆利用韩国企业直接投资的现状与展望

随着韩国企业在中国东部沿海发达地区的布局趋于完善和成熟以及中国西部大开发战略的逐步深入,全球经济格局调整和产业转移,大规模进入新疆等中国西部地区已成为必然。但目前韩国企业在新疆直接投资的整体规模上一直徘徊不前,相较东西部其他地区的投资额也有明显差距。因此,为了释放新疆与韩国的经济合作潜力,建议通过加强文化交流促进经济合作和改善投资环境加强直接投资的吸引力;通过吸引更多韩国跨国公司,扩大投资规模。     

工会维权在建设和谐企业中的重要作用

工会是企业连接员工的纽带,是职工利益的代表.在构建和谐企业过程中,工会应充分发挥其维权的独特作用.在整体维权、主动维权的前提下,具体应做到靠人维权、靠法维权、靠政策维权、靠合同维权、靠情理维权.     

边界功能、威胁认知与中国对陆上边界问题的应对

2017年6月,中印在洞朗地区发生边界对峙。中方在此次危机中始终保持了克制,并未通过武力方式解决边界危机。这与中国在1962年运用军事手段应对印度的挑衅截然不同。为何针对与同一个对象国出现的同类性质危机,中国的应对方式存在显著差异?文章认为,中国对陆上边界问题的处理方式,主要受到边界功能和决策者对威胁程度的认知这两个因素的影响。基于中国决策者对边界功能是政治与安全功能凸显还是经济功能优先,以及对对象国行为所带来的威胁认知高低程度不同的判断,将导致中国对边界问题采取四种应对方式:诉诸武力、展示决心(包括军事对峙等)、外交谈判、互利合作。通过对中国处理1962年与2017年两次中印边界危机、1956年中缅边界问题、2004年中俄边界问题的方式进行分析,可以发现上述分析框架得到了初步验证。文章通过探讨中国对陆上边界问题采取不同应对措施的原因,不仅有助于厘清影响中国政府针对陆上边界问题采取差异化应对措施的因素,还有利于为中国恰当地处理中印领土争端及其他陆上争议边界问题提供一定的启示,进而为改善中国周边外交、保障中华民族的伟大复兴提供初步的政策建议。     

Molecular Identification of Necrophagous Dermestes Species in South Korea Using Cytochrome c Oxidase Subunit I Nucleotide Sequences (Genus Dermestes)

Species identification of necrophagous insects found on a dead body is an essential key in applying medicolegal entomology to the estimation of postmortem interval (PMI). Due to limited morphological identification of insect evidence, several studies have identified species using molecular information such as DNA markers. While considerable cytochrome c oxidase subunit I (COI) gene sequence data of necrophagous fly species have been collected and annotated, those of necrophagous beetle species have not. Since necrophagous beetles such as Dermestes species have a larval period longer than that of flies, beetles are useful in even the late decomposition phase in estimating minimum PMI. To obtain the full-length COI gene sequences of six Dermestes species collected from South Korea, we designed primers for polymerase chain reaction amplification and sequencing. The obtained full COI nucleotide sequences were used for performing phylogenic analysis and comparison with previously reported sequences. The results demonstrated that the COI gene sequences could be used to identify forensically important Dermestes species in South Korea.