Trace DNA: a review,discussion of theory,and application of the transfer of trace quantities of DNA through skin contact

Advances in STR PCR DNA profiling technology allow for the analysis of minute quantities of DNA. It is frequently possible to obtain successful DNA results from cellular material transferred from the skin of an individual who has simply touched an object. Handling objects, such as weapons or other items associated with a crime, touching surfaces, or wearing clothing, may represent sufficient contact to transfer small numbers of DNA bearing cells, or trace DNA, which can be successfully analyzed. With this minimal amount of contact required to yield a suspect profile comes tremendous crime solving potential, and a number of considerations for prudent application, and the maximization of evidentiary value. Evidentiary materials not previously considered must be recognized and preserved, and the resulting DNA type profiles interpreted in their proper forensic context.     

Isotopic analogs as internal standards for quantitative GC/MS analysis--molecular abundance and retention time difference as interference factors

The following analyte/isotope-labeled internal standard (IS) systems are adapted to further study the interference phenomenon previously reported from our laboratory--the intensity ratio of the ion-pair designated for a specific analyte/2H-analog system increases as the solvent used to reconstitute the extraction/derivatization residue is increased: (a) Three analyte/2H-analog pairs with 2H-atoms positioned at allylic sites (butalbital, secobarbital, methohexital); (b) Two analyte/2H-analog pairs without these structural features (pentobarbital, phenobarbital); and (c) Two analyte/13C-analog pairs (butalbital, secobarbital). Major experimental parameters adapted in this study include: (a) Varying reconstitution solvent volume while keeping a constant analyte/IS concentration ratio; (b) Varying analyte/IS concentration ratio; (c) Varying gas chromatograph (GC) injection port temperature; and (d) Varying GC column temperature programming conditions, rendering difference in the degree of overlap of the peaks derived from the analyte and the 2H-analog. This study results in the following observations: (a) Changes in the intensity ratio of the ion-pair designated for a specific analyte/2H-analog system depend on molecular abundance, regardless of whether the 2H-atoms are positioned at active allylic positions or not--thus, ruling out hydrogen/deuterium exchange as the cause of the observed interference phenomenon; (b) Variations in GC injection port temperature do not alter the observed interference phenomenon-thus, ruling out chemical reactions at the injection port as the underlying cause; (c) Variations in peak-overlapping between the analyte and the 2H-analog, facilitated by changing GC column programming conditions, alter the observed interference phenomenon. Abundance of the analyte and the 2H-analog and their overlapping characteristic in the mass spectrometer ion source are believed to be the underlying cause of the observed interference phenomenon. The interference phenomenon observed for a specific analyte/2H-analog system has significant consequences on the linearity of the thereby generated calibration curves. Nonlinear approaches can better describe the calibration data and are needed more in comparison to systems in which 13C-analogs are used as the ISs.     

From Operational Strategy to Serving the Customer: Technology and Ethics in Welfare Law

We previously wrote critically on the UK's welfare computerization programme of the 1980s and in this article we investigate the latest programme. Our argument is that welfare is being seen and is being understood in a technological context rather than a truly welfare one— that is, that the technology behind the computerization projects model views of how welfare recipients should be processed. In the 1980s, the computer model that was being applied was one of data processing (i.e. the 'business model'). In the 2000s, it is the model of computer-based communication (i.e. the 'Internet model'). But further, the new technological communication model is being effused with an ethical aspect— that those who do not communicate are lacking in socio-ethical responsibility to society, and are deemed not to be truly living up to their role as citizens.     

Racist Offenders and the Politics of ‘Hate Crime’

In the UK and USA ‘Hate crime’ has become a topic of public controversy and social mobilization around issues of violence and harassment. This has largely but not exclusively addressed racism, homophobia and gender based violence. This article has three objectives. First, to situate hate crime legislation within a broad theory of modernity;secondly to examine the politics of its emergence as a public issue; thirdly to use data from the authors' recent research in Greater Manchester to illuminate the complexity of the concept of ‘hate crime’. The centrality of ‘hate crime’ to current debates derives from the importance of rights-based regulation of complex societies and the juridical management of emotional life. Hatred and violence have become problematic behaviour thrown into relief by a long term civilizing process. Hate crimes have thus acquired powerful rhetorical focus for mobilization of victim and identity politics. With reference to racist violence in Oldham and elsewhere in Greater Manchester, we argue that in its application and construction, however, ‘hate crime’ is a complex phenomenon that might dramatize rather than regulate the problems it seeks to address. This revised version was published online in July 2006 with corrections to the Cover Date.