Assessing a novel room temperature DNA storage medium for forensic biological samples

The ability to properly collect, analyze and preserve biological stains is important to preserving the integrity of forensic evidence. Stabilization of intact biological evidence in cells and the DNA extracts from them is particularly important since testing is generally not performed immediately following collection. Furthermore, retesting of stored DNA samples may be needed in casework for replicate testing, confirmation of results, and to accommodate future testing with new technologies.A novel room temperature DNA storage medium, SampleMatrix™ (SM; Biomatrica, Inc., San Diego, CA), was evaluated for stabilizing and protecting samples. Human genomic DNA samples at varying amounts (0.0625-200 ng) were stored dry in SM for 1 day to 1 year under varying conditions that included a typical ambient laboratory environment and also through successive freeze-thaw cycles (3 cycles). In addition, spiking of 1-4× SM into samples prior to analysis was performed to determine any inhibitory effects of SM. Quantification of recovered DNA following storage was determined by quantitative PCR or by agarose gel electrophoresis, and evaluation of quantitative peak height results from multiplex short tandem repeat (STR) analyses were performed to assess the efficacy of SM for preserving DNA.Results indicate no substantial differences between the quality of samples stored frozen in liquid and those samples maintained dry at ambient temperatures protected in SM. For long-term storage and the storage of low concentration samples, SM provided a significant advantage over freezer storage through higher DNA recovery. No detectable inhibition of amplification was observed at the recommended SM concentration and complete profiles were obtained from genomic DNA samples even in the presence of higher than recommended concentrations of the SM storage medium. The ability to stabilize and protect DNA from degradation at ambient temperatures for extended time periods could have tremendous impact in simplifying and improving sample storage conditions and requirements. The current work focuses on forensics analysis; however this technology is applicable to all endeavors requiring storage of DNA.     

When Local Comes to Town: Governing Local Agriculture in the South Carolina Lowcountry

The South Carolina Lowcountry—the coastal region centered on Charleston—has developed a vibrant local food system over the past several decades. This article examines the role of governance institutions in cultivating local-market farmers and the broader agricultural landscape. It argues that the region’s institutions of agricultural governance produce a farmer characterized by “entrepreneurial nostalgia”—put simply, the articulation of entrepreneurial and nostalgic subjects. This farmer subjectivity in many ways fits within what is generally deemed a neoliberal mode of being, yet this article also emphasizes, contrary to much of the work on neoliberalism, the racial politics of such a subject position. Entrepreneurial nostalgia not only emphasizes individualism and the marketing of the self but it does so in a way that aligns with both colorblind and liberal-multicultural forms of racism. The article closes by reflecting on potential openings for reworking this farmer subjectivity.     

Serious tax fraud and noncompliance

Research Summary This article reviews what international evidence exists on the impact of civil and criminal sanctions upon serious tax noncompliance by individuals. This construct lacks sharp definitional boundaries but includes large tax fraud and large-scale evasion that are not dealt with as fraud. Although substantial research and theory have been developed on general tax evasion and compliance, their conclusions might not apply to large-scale intentional fraudsters. No scientifically defensible studies directly compared civil and criminal sanctions for tax fraud, although one U.S. study reported that significantly enhanced criminal sanctions have more effects than enhanced audit levels. Prosecution is public, whereas administrative penalties are confidential, and this fact encourages those caught to pay heavy penalties to avoid publicity, a criminal record, and imprisonment. Policy Implications Although it has yet to be proven that prosecution has a greater or lesser impact on these offenders, increased prosecution might be justified for purposes of moral retribution as well as perceived social fairness.     

Slavery: a dual-equilibrium model with some historical examples

What sustains slavery, and why at critical junctures—the fall of the Roman Empire, the early modern expansion of plantation agriculture, the later phases of the industrial revolution, the totalitarian regimes of the 1930s—has it often expanded or contracted so rapidly? Why have elites sometimes been united, but sometimes violently divided, over the choice between free and servile labor? Why has slavery usually been ended by legal prohibition rather than voluntary abandonment? An extremely simple dual-equilibrium picture can illuminate how, when, and with whose support slavery is introduced or abolished. Internal divisions over slavery are likely to be most intense as a society approaches either of these “tipping points.” The most striking example, explored fleetingly here, is the US Civil War.     

The Financial Health of Political Parties in English Constituencies, 2004–05

Limited data have been available on the financial situation of the constituency branches of England's main political parties. Using recent annual accounts for all constituency parties with a turnover of £25,000 per annum or more in 2005, this paper reviews their financial health. It finds very little evidence of substantial financial turnover for the vast majority of local Labour parties and only a slightly better situation for the Liberal Democrats: many more local Conservative parties meet the reporting threshold but, even so, in almost half of all English constituencies none of the three parties reported a turnover of £25,000 or more. Local Conservative parties also differ substantially from the other two in their income sources and expenditure patterns.