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排序方式: 共有239条查询结果,搜索用时 31 毫秒
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J.J. Sanchez C. Brsting K. Balogh B. Berger M. Bogus J.M. Butler A. Carracedo D. Syndercombe Court L.A. Dixon B. Filipovi M. Fondevila P. Gill C.D. Harrison C. Hohoff R. Huel B. Ludes W. Parson T.J. Parsons E. Petkovski C. Phillips H. Schmitter P.M. Schneider P.M. Vallone N. Morling 《Forensic Science International: Genetics Supplement Series》2008,2(3):176-183
We report the results of an inter-laboratory exercise on typing of autosomal single nucleotide polymorphisms (SNP) for forensic genetic investigations in crime cases. The European DNA Profiling Group (EDNAP), a working group under the International Society for Forensic Genetics (ISFG), organised the exercise. A total of 11 European and one US forensic genetic laboratories tested a subset of a 52 SNP-multiplex PCR kit developed by the SNPforID consortium. The 52 SNP-multiplex kit amplifies 52 DNA fragments with 52 autosomal SNP loci in one multiplex PCR. The 52 SNPs are detected in two separate single base extension (SBE) multiplex reactions with 29 and 23 SNPs, respectively, using SNaPshot kit, capillary electrophoresis and multicolour fluorescence detection. For practical reasons, only the 29 SBE multiplex reaction was carried out by the participating laboratories. A total of 11 bloodstains on FTA cards including a sample of poor quality and a negative control were sent to the laboratories together with the essential reagents for the initial multiplex PCR and the multiplex SBE reaction. The total SNP locus dropout rate was 2.8% and more than 50% of the dropouts were observed with the poor quality sample. The overall rate of discrepant SNP allele assignments was 2.0%. Two laboratories reported 60% of all the discrepancies. Two laboratories reported all 29 SNP alleles in all 10 positive samples correctly. The results of the collaborative exercise were surprisingly good and demonstrate that SNP typing with SBE, capillary electrophoresis and multicolour detection methods can be developed for forensic genetics. 相似文献
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Rosalind Eyben Thalia Kidder Jo Rowlands Audrey Bronstein 《Development in Practice》2008,18(2):201-212
Development practice is informed by theories of change, but individuals and organisations may not make them explicit. Practitioners may be unaware of the extent to which strategic choices and debates are informed by disparate thinking about how history happens and the role of purposeful intervention for progressive social change. In the past few years, some Oxfam GB staff have been creating processes to debate their theories of change as part of an effort to improve practice. In this context, the authors introduce four sets of ideas about change, with a discussion of how they have been explored in two instances, and some of the challenges emerging from this process. Through explicitly debating theories of change, organisational decision-making processes can be better informed and strategic choices made more transparent. 相似文献
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Graeme Gill 《欧亚研究》2008,60(2):173-196
Symbols play an important role in the politics of all societies, especially at times of regime change when the symbols of the former regime are subject to three types of process: co-optation, contestation, and disavowal. An important symbol in post-communist Russia has been the body of Vladimir Lenin and, in particular, debate over whether it should remain in the mausoleum where it currently lies or whether it should be buried. Public opinion poll data throw some light on this, and enable us to evaluate the role that Lenin's body plays in contemporary politics in terms of notions of identity, legitimation, chronology and morality. 相似文献
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This article reviews current methodologies for the design of development projects and identifies foundational reasons for conflict between design approaches and participatory methods. A number of alternative approaches to the design of interventions in social systems are examined, and the potential application of some of these new ideas within a visioning process that is based on communicative rationality is explored. We conclude that there are many problems to be overcome before describing a complete design methodology which moves away from the objectivist basis of existing design systems, and that the new approach will need to address power relationships and the consequent and interrelated problems of accountability and trust. 相似文献
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