Screening Biological Stains with qPCR versus Lateral Flow Immunochromatographic Test Strips: A Quantitative Comparison using Analytical Figures of Merit

Biological fluid identification is an important facet of evidence examination in forensic laboratories worldwide. While identifying bodily fluids may provide insight into which downstream DNA methods to employ, these screening techniques consume a vital portion of the available evidence, are usually qualitative, and rely on visual interpretation. In contrast, qPCR yields information regarding the amount and proportion of amplifiable genetic material. In this study, dilution series of either semen or male saliva were prepared in either buffer or female blood. The samples were subjected to both lateral flow immunochromatographic test strips and qPCR analysis. Analytical figures of merit—including sensitivity, minimum distinguishable signal (MDS) and limit of detection (LOD)—were calculated and compared between methods. By applying the theory of the propagation of random errors, LODs were determined to be 0.05 μL of saliva for the RSID? Saliva cards, 0.03 μL of saliva for Quantifiler^® Duo, and 0.001 μL of semen for Quantifiler^® Duo. In conclusion, quantitative PCR was deemed a viable and effective screening method for subsequent DNA profiling due to its stability in different matrices, sensitivity, and low limits of detection.     

Assessing the impact of EU governmentality in post-conflict countries: pacification or reconciliation?

European Union (EU) interventions in conflict countries tend to focus on governance reforms of political and economic frameworks instead of the geopolitical context or the underlying power asymmetries that fuel conflict. They follow a liberal pattern often associated with northern donors and the UN system more generally. The EU's approach diverges from prevalent governance paradigms mainly in its engagement with social, identity and socio-economic exclusion. This article examines the EU's ‘peace-as-governance’ model in Cyprus, Georgia, Palestine and Bosnia and Herzegovina. These cases indicate that a tense and contradictory strategic situation may arise from an insufficient redress of underlying conflict issues.     

Philemon and Baucis Deaths: Case Reports and Postmortem Biochemistry Contribution

The death of a person as a reaction to the death of another is known as Philemon and Baucis death and may represent a difficult challenge for pathologists, especially due to the decompositional changes that often characterized these situations. In this article, we describe two cases of Philemon and Baucis deaths, the first concerning a married couple and the second concerning a father and son. Postmortem investigations including radiology and biochemistry were performed. Based on the results of all investigations, deaths were attributed to natural causes. Third‐party involvement could be excluded in both situations. In one case, biochemical analysis results revealed potentially contributing causes of death. The reported cases emphasize the importance of correlating findings from different postmortem investigations to identify causes of death, precipitating conditions and predisposing disorders, thus allowing the conclusion of natural death to be reached and third‐party involvement to be excluded.     

Sequence variation and allele nomenclature for the X-linked STRs DXS9895, DXS8378, DXS7132, DXS6800, DXS7133, GATA172D05, DXS7423 and DXS8377

X-linked DNA markers are increasingly used in forensic kinship testing. This paper presents sequencing data of the short tandem repeats (STRs) DXS9895, DXS8378, DXS7132, DXS6800, DXS7133, GATA172D05, DXS7423, DXS8377 and proposes an allele nomenclature. Alleles were assigned according to the recommendations of the International Society of Forensic Genetics (ISFG) Commission.     

Rapid Quantitative Analysis of Multiple Explosive Compound Classes on a Single Instrument via Flow‐Injection Analysis Tandem Mass Spectrometry

A flow‐injection analysis tandem mass spectrometry (FIA MSMS) method was developed for rapid quantitative analysis of 10 different inorganic and organic explosives. Performance is optimized by tailoring the ionization method (APCI/ESI), de‐clustering potentials, and collision energies for each specific analyte. In doing so, a single instrument can be used to detect urea nitrate, potassium chlorate, 2,4,6‐trinitrotoluene, 2,4,6‐trinitrophenylmethylnitramine, triacetone triperoxide, hexamethylene triperoxide diamine, pentaerythritol tetranitrate, 1,3,5‐trinitroperhydro‐1,3,5‐triazine, nitroglycerin, and octohy‐dro‐1,3,5,7‐tetranitro‐1,3,5,7‐tetrazocine with sensitivities all in the picogram per milliliter range. In conclusion, FIA APCI/ESI MSMS is a fast (<1 min/sample), sensitive (~pg/mL LOQ), and precise (intraday RSD < 10%) method for trace explosive detection that can play an important role in criminal and attributional forensics, counterterrorism, and environmental protection areas, and has the potential to augment or replace several of the existing explosive detection methods.     

How limited representativeness weakens throughput legitimacy in the EU: The example of interest groups

Are the interest groups (IGs) that constitute European umbrella organizations capable of cooperating in a way that contributes to the throughput legitimacy of the EU? To answer this question, the representativeness of supranational actors is developed as the central criteria of throughput legitimacy, thereby reconfiguring throughput legitimacy as a two‐level concept. Representativeness is operationalized as consisting of regular contacts between the constituent and the umbrella organization as well as the satisfaction of the former with the representation by the latter. The article looks empirically at agricultural, environmental and anti‐poverty groups. Whereas there are different degrees of contact depending on the policy area, the satisfaction with the representation by the umbrella is compromised for all three IGs. This is closely linked to lowest common denominator policies which reduce the representativeness of the umbrellas and therefore the throughput legitimacy of the EU, a problem that also exists for other actors of supranational governance.     

Network news coverage of terrorism: The early years

Abstract

The electronic media influence public opinion by defining which national and international issues become significant, and shape public debates by the television images conveyed to the American audience. Based upon the Vanderbilt Television News Archive, this study examines the image projected in reporting of terrorist activities by the three major national networks, the American Broadcasting Company (ABC), the Columbia Broadcasting System (CBS), and the National Broadcasting Company (NBC). The reality presented by the media is explored by comparing variables such as story emphasis and depiction, frequency, length of coverage, and location in the newscasts.     

Impossible Hosting: D’lo Sets an Undomesticated Stage for South Asian Youth Artists

At the center of this article is D'Lo, a Sri Lankan American hip hop performance artist, as the host of YouthWallah, a performance in Los Angeles, which features the work of South Asian youth artists. The focus of the article is on the possibility of an undomesticated stage that D'Lo creates for community youth artists as a female, yet unmistakably masculine host who translates female masculinity to a primarily straight South Asian audience as herself and, in drag, embodying her mother. D'Lo's performance includes autobiographical narratives of being gay and South Asian, which she uses to ground her politics in her experience. D'Lo does not perform the “exclusion” of a queer subject from a diasporic home. On the contrary D'Lo, as the host of this article home of YouthWallah 2004, provides an important undomesticated stage for the emergence of South Asian diasporic youth artists' voices and their alternative imaginations of diaspora. Therefore it becomes necessary to rethink the common conflation of notions of home and notions of domesticity, as a colonially inflected version of the home, which reproduces a colonial and gendered relation of power and thereby precludes the imagination of a decolonized community.