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861.
Schieltz DM McGrath SC McWilliams LG Rees J Bowen MD Kools JJ Dauphin LA Gomez-Saladin E Newton BN Stang HL Vick MJ Thomas J Pirkle JL Barr JR 《Forensic science international》2011,209(1-3):70-79
In late February 2008, law enforcement officials in Las Vegas, Nevada, discovered in a hotel room, a copy of The Anarchist Cookbook, suspected castor beans and a "white powder" thought to be a preparation of ricin. Ricin is a deadly toxin from the seed of the castor bean plant (Ricinus communis). The United States regulates the possession, use, and transfer of ricin and it is the only substance considered a warfare agent in both the Chemical and the Biological Weapons Conventions. Six samples obtained from the hotel room were analyzed by laboratories at the Centers for Disease Control and Prevention using a panel of biological and mass spectrometric assays. The biological assays (real time-PCR, time resolved fluorescence and cytotoxicity) provided presumptive evidence of active ricin in each of the samples. This initial screen was followed by an in-depth analysis using a novel, state-of-the-art mass spectrometry-based ricin functional assay and high sensitivity tandem mass spectrometry for protein identification. Mass spectrometric analysis positively identified ricin and confirmed that in each of the samples it was enzymatically active. The tandem mass spectrometry analysis used here is the most selective method available to detect ricin toxin. In each sample, ricin was unequivocally identified along with other R. communis plant proteins, including the highly homologous protein RCA120. Although database searches using tandem mass spectra acquired from the samples indicated that additional controlled substances were not present in these samples, the mass spectrometric results did provide extensive detail about the sample contents. To the best of our knowledge following a review of the available literature, this report describes the most detailed analysis of a white powder for a public health or forensic investigation involving ricin. 相似文献
862.
Rick Anderson 《Journal of Public Affairs (14723891)》2023,23(4):e2875
This contribution concerns a study that aims to trace factors that determine the effectiveness of policy implementation. To this end, a case study was conducted in two municipalities. In both municipalities, a random sample was drawn of 100 activities in the cultural field carried out in the year 2021. Based on this, the effectiveness of the implementation of subsidy provision was examined for both municipalities. This effectiveness has been operationalised to the percentage of correct non-applications, the percentage of rightly approved subsidy applications and the percentage of rightly rejected subsidy applications. A comparison between these municipalities, based on an analysis of the documents and 22 interviews, then provided an opportunity to explain differences in effectiveness. It turns out, that the control function provides the decisive explanation for the difference in effectiveness. This control function is not only about monitoring and controlling the implementation, but also about facilitating and optimising the process of providing subsidies. Although previous research highlights the importance of an adequate monitoring and control for implementation, the control function can be defined in a broader way. Besides overseeing proper implementation, the control function in this broader view also plays a facilitating role towards the implementers. This broader control function specifically has not often been the explicit object of implementation research. It should be noted, however, that this study is based on only two municipalities, which only examined the subsidisation of cultural activities. Therefore, no general conclusions can be drawn on this basis. 相似文献