A systematic regional study of dopamine and dopamine-derived salsolinol and norsalsolinol levels in human brain areas

Dopamine and the dopamine-derived tetrahydroisoquinoline alkaloids salsolinol and norsalsolinol were measured by high-performance liquid chromatography with electrochemical detection in 15 regions of the human brain. The regional distribution of dopamine in 32 brains was similar to previous reports with highest concentrations in the basal ganglia, especially in the striatum, followed by the substantia nigra and the hypothalamus. Significant amounts of salsolinol and norsalsolinol were only found in these dopamine-rich areas, whereas in the other regions no alkaloids were detected. These findings suggest that the concentration of the substrate dopamine may determine the alkaloid level during in vivo formation.     

Frozen: Thawing and Its Effect on the Postmortem Microbiome in Two Pediatric Cases,

Previous postmortem microbiome studies have focused on characterizing taxa turnover during an undisturbed decomposition process. How coexisting conditions (e.g., frozen, buried, burned) affect the human microbiome at the time of discovery is less well understood. Microbiome data were collected from two pediatric cases at the Wayne County Medical Examiner in Michigan. The bodies were found frozen, hidden in a freezer for an extended time. Microbial communities were sampled from six external anatomic locations at three time points during the thawing process, prior to autopsy. The 16S rRNA V4 gene amplicon region was sequenced using high‐throughput sequencing (Illumina MiSeq). Microbial diversity increased, and there was a distinct shift in microbial community structure and abundance throughout the thawing process. Overall, these data demonstrate that the postmortem human microbiome changes during the thawing process, and have important forensic implications when bodies have been substantially altered, modified, and concealed after death.     

Public Smart Service Provision in Smart Cities: A Case-Study-Based Approach

ABSTRACT

In view of the multitude of challenges urban public administration is struggling with, more and more cities are turning towards smart solutions using information and communication technology. Such Smart City approaches revert to data provided in the urban space by sensors, by citizens or by the archives of the authorities aiming at providing public services that increase the quality of life for citizens, optimize mobility within the city or enhance flexibility and responsiveness of public authorities. This article identifies such SC approaches as well as their basic technical requirements for the cities of Vienna, Singapore, and San Francisco and, by compiling the public service portfolios of these cities, creates an integrative, strategic overview of a SC’s range of services. This approach thus helps to create a scientific basis for the evaluation of SC services and to provide a strategic modular toolkit for public managers to plan and design SCs.     

Commotio cordis: A case report of a fatal blow

Commotio cordis secondary to a blunt blow to the chest wall can result in ventricular fibrillation and sudden death in children. While it is commonly reported in adolescents during sporting activities, it may result from non-accidental trauma especially in infants and younger children. We report a case of a 6-month-old baby boy who presented to the emergency department in cardiac arrest. The patient's hospital records, postmortem imaging, and the autopsy results were reviewed. External examination of the infant did not reveal any evidence of trauma. Postmortem imaging revealed multiple healing posterior rib fractures and a metaphyseal corner fracture, both considered fractures highly specific for physical abuse. The autopsy revealed a structurally normal heart with no microscopic abnormalities. The infant's father confessed to hitting the child on the chest after which the child became unresponsive. Given the constellation of postmortem imaging and autopsy findings in addition to the father's confession, the child's death was ruled as a homicide secondary to commotio cordis. Since there are no structural and microscopic abnormalities in the heart autopsy in cases of commotio cordis, timely on-scene investigation and a thorough investigation regarding the mechanism of injury are required to make this diagnosis. Early identification of non-accidental trauma is crucial and can prevent further abuse in other siblings.     

Morphology of low-velocity impact stains produced from single drops of blood

Systematic variation of blood droplet volume, the distance fallen and the surface (paper, wood, plastics, tiles) led to the conclusion that the size and the shape of the stains ("fingers", satellites) allowed to deduce the distance fallen but only if the actual surface structure was known. We found that detailed photography at the crime scene was necessary, yet experiments have to be performed due to the extreme influence of the actual surface texture on all characteristics (size, spines, peripheral spatter) of the blood stains.     

Suicide by self-immolation

In the Western world, self-incineration is a rare event compared with the Asian countries. The circumstances of death are often unusual, and differentiation from homicide is sometimes possible only after evaluating all the investigation results and findings. In many cases of self-cremation, a fire accelerant is used. In our study material at least 30% of the body surface were burned. Unaffected skin was predominantly found on the back of the body. The cases presented by us are inhomogenous as to the motive and differ from those reported in the literature with regard to gender distribution, scene, motivational situation and psychological constitution.     

Engineering a critical realignment of the electorate: The case of the socialists in France

For the first time, largely owing to the Socialists' ability to gain and retain the allegiance of the electorate, France has experienced a major electoral realignment without a concomitant change in governmental system. The Socialists' success results from three major factors: their pre‐1981 electoral strategy which involved refashioning their traditional ideology and rhetoric to appeal to a new, emerging portion of the electorate, the wage‐earners; their subsequent ideological flexibility, which included a move toward more pragmatic politics along with an opening to the centre; and the failure of their rivals on the moderate and the extreme right to mount a lasting challenge with their own counter‐ideologies and policy initiatives.